pmc logo image
Logo of jbacterJ Bacteriol SubscriptionsJ Bacteriol Web Site
Journal List > J Bacteriol > v.111(3); Sep 1972

Formats:
J Bacteriol. 1972 September; 111(3): 641–648.
PMCID: PMC251335
Copyright notice
Allostery of 3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthetase in Clostridium: Another Conserved Generic Characteristic
Roy A. Jensen1 and Robert Twarog2
Department of Microbiology, Baylor College of Medicine, Houston, Texas 77025
2Department of Bacteriology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514
Abstract
Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. acetobutylicum, and C. tetanomorphum. Quantitative differences in the enzymology and kinetics of allosteric control distinguished C. tetanomorphum from C. butyricum and C. acetobutylicum. Crude extracts contained apparent proteolytic activity which could be fractionated from DAHP synthetase. The proteolytic activity was more labile than DAHP synthetase in extracts and was progressively inactivated by serial freeze-thaw treatments. Protease activity was at least partially inhibited by phenylmethylsulfonyl-fluoride. The method of comparative allostery of DAHP synthetase distinguishes the genera Bacillus and Clostridium, each having a strongly conserved pattern of regulation for DAHP synthetase. The data reinforce previous conclusions that allosteric control patterns governing the activity of DAHP synthetase are stable, reliable generic characteristics.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 641
641
icon of scanned page 642
642
icon of scanned page 643
643
icon of scanned page 644
644
icon of scanned page 645
645
icon of scanned page 646
646
icon of scanned page 647
647
icon of scanned page 648
648
 
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • COHEN-BAZIRE G, SISTROM WR, STANIER RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. [PubMed]
  • De Ley J. Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol. 1970 Mar;101(3):738–754. [PubMed]
  • Foubert EL, Douglas HC. Studies on the Anaerobic Micrococci: II. The Fermentation of Lactate by Micrococcus lactilyticus. J Bacteriol. 1948 Jul;56(1):35–36. [PubMed]
  • Hill LR. An index to deoxyribonucleic acid base compositions of bacterial species. J Gen Microbiol. 1966 Sep;44(3):419–437. [PubMed]
  • Jensen RA. Taxonomic implications of temperature dependence of the allosteric inhibition of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase in Bacillus. J Bacteriol. 1970 May;102(2):489–497. [PubMed]
  • Jensen RA, Nasser DS, Nester EW. Comparative control of a branch-point enzyme in microorganisms. J Bacteriol. 1967 Nov;94(5):1582–1593. [PubMed]
  • JENSEN RA, NESTER EW. THE REGULATORY SIGNIFICANCE OF INTERMEDIARY METABOLITES: CONTROL OF AROMATIC ACID BIOSYNTHESIS BY FEEDBACK INHIBITION IN BACILLUS SUBTILIS. J Mol Biol. 1965 Jun;12:468–481. [PubMed]
  • Jensen RA, Nester EW. Regulatory enzymes of aromatic amino acid biosynthesis in Bacillus subtilis. I. Purification and properties of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase. J Biol Chem. 1966 Jul 25;241(14):3365–3372. [PubMed]
  • Jensen RA, Stenmark SL. Comparative allostery of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase as a molecular basis for classification. J Bacteriol. 1970 Mar;101(3):763–769. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Mead GC. The amino acid-fermenting clostridia. J Gen Microbiol. 1971 Jul;67(1):47–56. [PubMed]
  • Nester EW, Jensen RA. Control of aromatic acid biosynthesis in Bacillus subtilis: sequenial feedback inhibition. J Bacteriol. 1966 Apr;91(4):1594–1598. [PubMed]
  • Richmond RC. Non-Darwinian evolution: a critique. Nature. 1970 Mar 14;225(5237):1025–1028. [PubMed]
Articles from Journal of Bacteriology are provided here courtesy of
American Society for Microbiology (ASM)

PubMed articles by these authors