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Journal List > J Bacteriol > v.111(1); Jul 1972

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J Bacteriol. 1972 July; 111(1): 112–118.
PMCID: PMC251247
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Documentation of Auxotrophic Mutation in Blue-Green Bacteria: Characterization of a Tryptophan Auxotroph in Agmenellum quadruplicatum
Lonnie O. Ingram, Duane Pierson, James F. Kane,1 C. Van Baalen, and Roy A. Jensen
aMarine Science Institute, University of Texas, Port Aransas, Texas 78373; The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830; and Department of Microbiology, Baylor College of Medicine, Houston, Texas 77025
1 Present address: Department of Microbiology, University of Tennessee Medical Units, Memphis, Tenn. 38103.
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Abstract
A tryptophan-requiring auxotroph of Agmenellum quadruplicatum strain BG1, a species of blue-green bacteria, was isolated by means of a nitrosoguanidine-penicillin procedure. Its growth characteristics were determined, and the enzymological block was identified in the A activity of tryptophan synthetase. Starvation of the auxotroph for tryptophan resulted in the derepression of the synthesis of all five enzymes. The first four enzymes derepressed 2- to 3-fold, and tryptophan synthetase B derepressed 20-fold. In the parental prototroph, BG1, anthranilate synthetase was active in crude extracts with ammonia as the amino donor reactant, but not with glutamine.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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