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J Evol Biol. Author manuscript; available in PMC Aug 11, 2008.
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PMCID: PMC2505272

Widespread unidirectional transfer of mitochondrial DNA: a case in western Palearctic water frogs


Interspecies transfer of mitochondrial (mt) DNA is a common phenomenon in plants, invertebrates, and vertebrates, normally linked with hybridization of closely related species in zones of sympatry or parapatry. In Central Europe, in an area north of 48° N latitude and between 08° and 22° E longitude, western Palearctic water frogs show massive unidirectional introgression of mtDNA: 33.7% of 407 R. ridibunda possessed mtDNA specific for R. lessonae. In contrast, no R. lessonae with R. ridibunda mtDNA was observed. That R. ridibunda with introgressed mitochondrial genomes were found exclusively within the range of the hybrid R. esculenta and that most hybrids had lessonae mtDNA (90.4% of 335 individuals investigated) is evidence that R. esculenta serves as a vehicle for transfer of lessonae mtDNA into R. ridibunda. Such introgression has occurred several times independently. The abundance and wide distribution of individuals with introgressed mitochondrial genomes show that R. lessonae mt genomes work successfully in a R. ridibunda chromosomal background despite their high sequence divergence from R. ridibunda mtDNAs (14.2–15.2% in the ND2/ND3 genes). Greater effectiveness of enzymes encoded by R. lessonae mtDNA may be advantageous to individuals of R. ridibunda and probably R. esculenta in the northern parts of their ranges.

Keywords: clonal reproduction, hybridization, hybridogenesis, interspecies introgression, mtDNA, Rana esculenta complex


Interspecies transfer of mitochondrial (mt) DNA has been detected in a variety of organisms, for example in flowering plants (e.g. Bergthorsson et al., 2003), invertebrates (e.g. Croucher et al., 2004; Abe et al., 2005; Bachtrog et al., 2006), and among vertebrates in fish (e.g. Yamada et al. 2001, Sullivan et al. 2004), amphibians (e.g. Babik et al., 2005), birds (e.g. Dabrowski et al. 2005) and mammals (e.g. Ruedi et al., 1997, Fredsted et al., 2006). In most cases introgressed mtDNA was found in very closely related species (especially in sister species) and was directly linked to interspecies hybridization in zones of sympatry or parapatry. Such genome transfer is always mediated by fertile or at least partially fertile hybrids that transmit their maternal mtDNA to the paternal gene pool via backcrosses with males of the paternal parental species. In some populations introduced foreign mtDNA can completely replace the resident mtDNA either through genetic drift or natural selection (Ballard & Whitlock, 2004). The detection and analysis of introgressive hybridization is not only important for taxonomic purposes and phylogenetic inference, it may also provide fundamental insights into evolutionary processes, especially for studying the role of nuclear/mitochondrial co-adaptation in speciation, including hybrid breakdown and reproductive isolation (e.g., Ballard & Whitlock, 2004; Burton et al., 2006).

The western Palearctic water frog group offers significant opportunities to study these problems because it comprises several evolutionary species that are not reproductively isolated. There are large areas of sympatry in which fertile hybrids are formed (reviewed by Plötner, 2005). At least three of the known hybrid forms reproduce hemiclonally by a hybridogenetic (Schultz, 1969) gametogenesis: in their germ line one parental genome is excluded while the remaining one is endoreduplicated prior to meiosis (reviewed by Graf & Polls Pelaz, 1989; Plötner, 2005).

The best-studied hybrid form is Rana esculenta (genomic composition LR) which originated from natural crosses between Rana lessonae (LL) and Rana ridibunda (RR). In the germ line of this hybrid, one parental genome (usually L, sometimes R) is excluded and haploid gametes (ova or sperm) are produced that contain an intact, unrecombined genome of the other parental species (reviewed by Graf & Polls Pelaz, 1989; Plötner, 2005). Such hybrid lineages in most cases coexist with and reproductively depend on one parental species. Matings with that species replace the genome lost during gametogenesis and restore somatic hybridity in each hybrid generation. In the widespread and abundant lessonae-esculenta (L-E) system, in which both male and female hybrids occur, R. esculenta usually coexist, as sexual parasites, with R. lessonae and exclude the L genome prior to meiosis. In populations of the less frequent ridibunda-esculenta (R-E) system, in which hybrids are only males, they live together with R. ridibunda and usually exclude the R genome (Uzzell & Berger, 1975; Uzzell et al., 1977; Günther & Plötner, 1988). In addition to diploid LR hybrids, some water frog populations, especially in north-eastern Germany and north-western Poland, contain triploid (3N) hybrids (Günther, 1975, 1983; Rybacki & Berger, 2001; Plötner, 2005) that normally have either two R. lessonae genomes and one R. ridibunda genome (genotype LLR) or one R. lessonae genome and two R. ridibunda genomes (genotype RRL). They usually exclude the rarer genome and form unrecombined haploid gametes (Günther et al., 1979; Günther, 1983; Berger & Günther, 1988; Vinogradov et al., 1991).

For size-related behavioral reasons, natural primary hybridizations between R. ridibunda and R. lessonae occur between the large females of R. ridibunda and the small males of R. lessonae rather than the reciprocal (Berger, 1970; Borkin et al., 1979). As in most vertebrates, frog mtDNA is inherited strictly from the mother, so all newly formed R. esculenta lineages contain R. ridibunda mtDNA. In the L-E population system, most matings take place between R. esculenta females and R. lessonae males, but successful matings occur also in the reciprocal combination (Blankenhorn, 1977; Lengagne, et al. 2006). Each such mating between an R. lessonae female and an R. esculenta male generates R. esculenta lineages with R. lessonae mtDNA. In the absence of the parental species R. ridibunda, such a transfer of R. lessonae mtDNA into a hybridogenetic lineage is irreversible, and indeed, most hemiclonal hybrids of the L-E system contain mtDNA of their local host parental species rather than R. ridibunda mtDNA, a result of such occasional matings of host females with hybrid males (Spolsky & Uzzell 1986). With repeated formation of R. esculenta lineages containing R. lessonae mtDNA and chance losses of lineages containing R. ridibunda mtDNA, all hybrid individuals in L-E system populations come to have R. lessonae mtDNA. When such hemiclonally-reproducing hybrid lineages occur within the range of R. ridibunda, however, they provide a vehicle for interspecific introgression of R. lessonae mtDNA into R. ridibunda. Matings between female R. esculenta with R. lessonae mtDNA and male R. ridibunda (or R. esculenta) lead to R. ridibunda progeny of both sexes (or females only) with R. lessonae mtDNA (Spolsky & Uzzell, 1984; Hotz et al., 1992).

Three geographical ranges are relevant to the introgression of R. lessonae mtDNA into R. ridibunda: (1) The area of sympatry of R. ridibunda and R. lessonae, because it defines the region where primary hybridizations between them can occur. Such sympatry presently exists from northern Ukraine and western Russia to eastern Austria and central Germany (Günther 1990; 1997a,b). (2) The area in which R. ridibunda genomes in hybrids between R. lessonae and R. ridibunda lead to germ line exclusion of the L genome and thus found hemiclonally-reproducing hybrid lineages rather than non-hybridogenetic sterile F1 hybrids. Here, R. esculenta lineages can constitute vehicles for the introgression of R. lessonae mtDNA into R. ridibunda. This area currently comprises most parts of the sympatry region (Central and eastern Europe), but excludes an eastern zone from northern Ukraine to the Danube delta in eastern Romania. In this zone and in more southern parts of the R. ridibunda range, the R. ridibunda genome does not induce hemiclonal gametogenesis in hybrids with R. lessonae (cf. Hotz et al., 1985; Bucci et al., 1990; Günther et al., 1991, Berger et al., 1994, unpublished data). (3) Areas in which the sex ratio in R. esculenta hinders regular matings between female R. lessonae and male R. esculenta; such matings are necessary to transfer R. lessonae mtDNA into R. esculenta lineages and via these lineages into R. ridibunda. Whereas R. esculenta lineages in most parts of Central Europe contain both sexes, usually with a slight excess of females, they consist almost exclusively (~97%) of females in many populations of the Pannonian Basin (eastern Austria, northern Bosnia and Herzegovina, northeastern Croatia, Hungary, northern Serbia, southern lowlands of Slovakia, northeastern Slovenia, western Romania, and Ukraine west of the Carpathian Mountains; Berger et al., 1988; Gubányi, 1992; Morozov-Leonov et al., 2003; unpublished data), and in the Rostov Province of Russia (Borkin et al., 2006).

We examined mtDNA of water frogs from 194 localities throughout and beyond the sympatry area of R. ridibunda and R. lessonae, determining species origin and haplotype of mtDNA. We asked how often and where the introgression of R. lessonae mtDNA into R. ridibunda occurred, and addressed the geographic relation of the region of R. lessonae mtDNA introgressed into R. ridibunda with (1) the sympatry area of R. ridibunda and R. lessonae; (2) the area in which hybrids reproduce hemiclonally; and (3) the area in which hybrids are almost exclusively females.

Material and Methods

Sampling, taxon designation, and ploidy determination

Localities of 407 R. ridibunda, 117 R. lessonae, 335 R. esculenta, and two individuals of uncertain identification (R. lessonae or R. esculenta) are listed in Appendix S1 (Supplementary Material online). We use the name R. ridibunda for individuals distributed in Europe, and include frogs currently known as Rana kurtmuelleri. Unpublished molecular data suggest that individuals from the type locality of R. ridibunda (north coast of the Caspian Sea) may not be conspecific with individuals from Europe, in which case the oldest available name for the European frogs is R. fortis Boulenger, 1884 (Dubois & Ohler, 1994). Except for two individuals from France (localities 31 and 33), for which we used DNA sequences for haplotype comparisons and sequence statistics, R. ridibunda from France and Switzerland were not considered because all lake frog populations in these countries trace back to individuals introduced not only from other European regions but also from Anatolia (Grossenbacher, 1988; Pagano et al., 2003; Plötner & Ohst, unpublished results).

Specimens were assigned to taxa on the basis of their external morphology; Günther (1990) and Plötner (2005) described diagnostic characters. Ploidy was determined by measurements of erythrocytes based on the finding that triploid (3N) erythrocytes are on average 30% larger than diploid (2N) ones (Uzzell & Berger, 1975; Günther, 1977). The kind of mtDNA present in all individuals in our sample was determined by one or more methods: sequencing of ND2, ND3, and parts of 12S rRNA gene, endonuclease restriction of whole mtDNA, AluI restriction of a PCR-amplified segment of the ND2 gene. Our sequence data are supplemented by published restriction fragment analyses (Spolsky & Uzzell, 1984, 1986).

DNA extraction, PCR conditions, sequencing

Genomic DNA was isolated from blood, muscle and liver tissue using QIAmp blood and tissue kits (QIAGEN GmbH, Hilden, Germany) according to the company's protocols. Before DNA isolation, ethanol preserved material was soaked in three changes of distilled water over a 48 h period. Double stranded amplification and cycle sequencing of mtDNA (1038 bp of ND2, 340 bp of ND3, 374 bp of 12S rDNA) were carried out as described in Appendix S2 (Supplementary Material online). The sequenced sites correspond to positions 6546–7583 (ND2), 12089–12428 (ND3), and 3156–3528 (12S) in the genome of Rana nigromaculata (AB043889, Sumida et al., 2001). Amplified DNA was purified using QIAquick PCR purification kits (QIAGEN). A Big Dye Ready Reaction Terminator Cycle Sequencing Kit (Applied Biosystems) was used to perform the sequencing reaction. Electrophoresis and detection of fluorescent dye labelled nucleotide fragments was done with an automated DNA sequencer (ABI Prism 377). All sequences were deposited in the EMBL Nucleotide Sequence Database under the accession numbers listed in Appendix S1 (Supplementary Material online).

Restriction fragment analysis (RFA)

Isolation and purification of mtDNA followed standard methods (Spolsky & Uzzell, 1984, 1986). Approximately 5–10 ng of each DNA were digested to completion with 19 different restriction enzymes (AvaI, BamHI, BclI, BglII, EcoRI, EcoRV, HaeII, HincII, HindIII, HpaI, KpnI, MluI, PstI, PvuII, SalI, SmaI, SstI, XbaI, XhoI) under conditions recommended by the supplier (Boeringer Mannheim Biochemicals, Bethesda Research Laboratories, or New England Biolabs). Resulting DNA fragments were end-labeled with a mixture of four α-32-P-triphosphate deoxynucleosides (adenine, cytosine, guanine, and tyrosine). Restriction-enzyme-fragment patterns were determined from autoradiographs after electrophoresis through horizontal 1% agarose or vertical 3.5–4% polyacrylamide gels. For each gel, fragment sizes were estimated from mobilities of size standards (λ and PM2 DNAs, each cut with HindIII for agarose gels; ϕX174 cut with HincII and pBR322 cut with AluI for polyacrylamide gels).

AluI restriction of ND2 PCR products

Mitochondrial ND2 sequences of 287 western Palearctic water frogs representing at least ten evolutionary species were analyzed with the online tool WEB CUTTER (www.medkem.gu.se/cutter) to find specific restriction sites that allow differentiation between R. lessonae and R. ridibunda mtDNAs. One AluI restriction site (AG↓CT) spanning positions 633–636 was detected in each of the 44 R. ridibunda ND2 sequences, in seven ND2 sequences of R. bedriagae from the Near East (western Syria, Jordan, Nile delta) and in nine of R. cretensis but not in the ND2 sequences of 56 R. lessonae or any of other western Palearctic water frogs (unpublished results). To distinguish between R. ridibunda and R. lessonae specific mitochondrial genomes only a 770 bp long mtDNA segment that contained this restriction site was amplified using the primers L2 and H2 (Appendix S2, Supplementary Material online). 2 µl (20 units) AluI (Boehringer Mannheim [BM] or Angewandte Gentechnologie Systeme GmbH [AGS]), 2 µl supplied incubation buffer (BM incubation buffer A or AGS buffer violet), and 1 µl double distilled water were added to 15 µl of the solution that contained the PCR product. After incubation for 2 h at 37 °C, the fragments were separated by electrophoresis on a horizontal 2% agarose gel. R. ridibunda-specific mtDNA was cut into two fragments with lengths of 319 bp and 451 bp, whereas mtDNA of R. lessonae remained undigested as evidenced by a single band in the gel.

Data analysis

Mitochondrial DNA sequences were initially aligned using CLUSTAL V (Higgins et al., 1992). Subsequently, alignments were improved manually. They are available from the EMBL align database under the accession number ALIGN_001211. For sequence statistics the programs MEGA 3.1 (Kumar et al., 2004) and DnaSP 4.10.9 (Rozas et al., 2003) were used. We calculated the total number of transitions, transversions, and amino acid substitutions, the nucleotide diversity (Pi), and uncorrected p distances (a simple measure of sequence divergence). We applied Tajima’s D-test (Tajima, 1989) to investigate departures from selective neutrality at the ND2 and ND3 genes in both R. ridibunda and R. lessonae, and a McDonald-Kreitman (MK) test (McDonald & Kreitman, 1991) to test for neutrality in interspecies sequence divergence. The neutrality index (NI) was calculated using Rand & Kann’s (1996) formula. The restriction profiles were analyzed by calculating the amount of sequence divergence from the fraction of restriction fragments shared by a pair of mtDNAs (Nei & Li, 1979), using Upholt’s (1977) formula.

Mitochondrial haplotype genealogies were estimated from complete ND2 and ND3 sequences with maximum likelihood (PAUP 4.0b10: Swofford, 2003) and Bayesian algorithms (MrBayes 3.1.2: Ronquist & Huelsenbeck, 2003; Huelsenbeck & Ronquist, 2005). For both maximum likelihood and Bayesian inference, the general time reversible model with corrections for invariant characters and gamma distributed rate heterogeneity (GTR+I+G) was applied as recommended by Huelsenbeck & Rannala (2004). Model parameters were estimated from the data set. For MrBayes, the posterior probabilities of phylogenetic trees were calculated by employing a 107-generation Metropolis-coupled Markov chain Monte Carlo (4 chains, chain-temperature parameter=0.2) as implemented in the program. Trees were sampled every 1000 generations; the first 1000 recorded trees were discarded; a total of 9000 recorded trees was examined. The autocorrelation behavior of MrBayes was explored with program-internal statistics and the tool AWTY (http://ceb.fsu.edu/awty; Nylander et al., 2007). Both analyses suggested convergence of the split frequencies (standard deviation after 3500 recorded trees was lower than 0.01). The convergence of the model parameter estimates was checked using the potential scale reduction factor calculated in MrBayes: the limit of the statistic of 1.0 was reached for all model parameters.

For the phylogenetic analysis we augmented the R. ridibunda and R. lessonae sequences with sequences of R. epeirotica and R. cretensis as an outgroup, and R. shqiperica, the presumptive sister species of R. lessonae sensu lato. This allowed a consistent rooting of the haplotypes for R. ridibunda and R. lessonae on the tree.

To analyze the effects of location (longitude, latitude) on haplotype composition in R. esculenta and on the introgression rate of R. lessonae-specific mtDNA into R. ridibunda, we calculated the relative frequency (fl) of R. lessonae-specific haplotypes (including haplotypes of the Italian R. lessonae stock) for each population with sample size larger than one. We performed simple and multiple regression analyses with fl as the dependent variable and latitude and longitude as independent variables using the software packet Statgraphics Plus 4.1 (Statistical Graphics Corp.). To judge the relative magnitude of the residuals with respect to the explanatory power of the selected independent variables, a component-plus-residuals plot (Wood, 1973) was made. In the regression analyses we also incorporated data published by (Spolsky & Uzzell 1984, 1986) and Monnerot et al. (1985) (Appendix S1, Supplementary Material online).


Inter- and intraspecific variation of mtDNA

Both mitochondrial sequences (ND2, ND3, 12S rDNA) and digest profiles of whole mtDNAs revealed clear differences between R. ridibunda and R. lessonae. Interspecific comparisons of the 12S rRNA gene segments yielded substitution numbers between 9 and 12, which correspond to sequence divergences of 2.4% and 3.2%, respectively (Plötner & Ohst, 2001). The interspecific differences in the less conserved ND2+ND3 sequences are about five to six times higher than in the 12S rRNA gene segment. Nevertheless, because there are at least nine substitutions, the species-specificity of mtDNA could be clearly determined on the basis of this gene segment.

The R. ridibunda ND2+ND3 sequences contained 38 (2.8%) variable sites (31 in ND2, seven in ND3) defining 19 haplotypes (Table 1, Fig. 1); 31 (2.3%) sites were parsimony informative. In both genes almost all nucleotide substitutions were transitions, most of them at the third codon position; only three were found in the first position (two in ND2, one in ND3) and two in the second position (both in ND2). Only five substitutions resulted in amino acid replacements (four in ND2, one in ND3). Nucleotide diversity (Pi) amounted to 0.007±0.0005. Genetic distance ranged from 0.07 to 1.6% among R. ridibunda-specific haplotypes with an overall nucleotide diversity of 0.74%.

Fig. 1
Phylogenetic relationships of R. lessonae and R. ridibunda haplotypes. (A) Complete MrBayes maximum posterior tree of all haplotypes with R. shqiperica included as the sister taxon of R. lessonae and two presumptive outgroups of R. ridibunda (R. cretensis ...
Table 1
Variable positions and nucleotide composition in the mt ND2 and ND3 gene defining 19 different haplotypes (HT) across 29 populations of R. ridibunda. Transversions are indicated by asterisks. The numbers below nucleotide positions indicate the codon position. ...

In the ND2+ND3 sequences of R. lessonae, the intraspecific variability is about 2.5 times lower than in R. ridibunda. Intraspecific p distances ranged between 0.1 and 0.7% with an average of 0.3%. Only 24 variable sites (1.7%) were found (21 in ND2, three in ND3); almost all substitutions were transitions at the first (10) or third (11) codon position (Table 2). Seven sites (0.5%) were parsimony informative. Twelve amino acid replacements were observed caused by eleven substitutions in the first codon position and one substitution in the second codon position. A Pi-value of 0.00187± 0.00033 was calculated.

Table 2
Variable positions and nucleotide composition in the mt ND2 and ND3 gene defining 17 different haplotypes (HT) across 39 populations of R. lessonae. Transversions are indicated by asterisks. The numbers below nucleotide positions indicate the codon position. ...

A total of 17 R. lessonae-specific haplotypes could be distinguished (Table 2), which represent three main clusters as supported by the results of maximum likelihood (data not shown) and Bayesian analysis (Fig. 1). Cluster 1 comprises the very closely related haplotypes L1–L12: cluster 2 consists of only one haplotype (L13), which perhaps represents an ancestral state within the R. lessonae clade; and cluster 3 includes haplotypes L14–L17, which differ from the other R. lessonae-specific haplotypes by at least four substitutions (three in ND2, one in ND3).

The digest profiles of R. ridibunda and R. lessonae mtDNAs were described in detail by (Spolsky & Uzzell 1984, 1986). The proportion of fragments shared by both species ranged between 0.256 and 0.258, which corresponds to sequence differences of 8.1±1.0% and 8.2±1.0%, respectively. In the ND2 gene, R. ridibunda differed from R. lessonae in at least 134 sites (12.9%); in the ND3 gene a minimum of 48 substitutions (14.1%) were detected. The sequence divergence (p distance) obtained as a result of interspecific comparisons of the complete ND2+ND3 sequences ranged between 14.2% and 15.2%.

Compared to the 36 mitochondrial haplotypes differentiated on the basis of ND2+ND3 sequences (Table 1 and and2),2), only three types of mtDNAs (A–C) were detected by restriction fragment patterns of total mtDNA (Spolsky & Uzzell, 1984). Type A was found in R. ridibunda and R. esculenta but not in R. lessonae and thus was considered as R. ridibunda-specific. Type A probably contains several of the 19 ND2+ND3 haplotypes; because there are no differences in the recognition sites in the ND2 and ND3 genes of R. ridibunda for the restriction enzymes used by Spolsky & Uzzell, the ND2+ND3 sequence diversity would not have been revealed in their study.

Types B and C, which were considered as R. lessonae-specific, showed a sequence divergence of only 0.3%, consistent with the p distances obtained from intraspecific comparisons of the ND2 and ND3 sequences (0.1–0.7%). One BamHI restriction site (G↓GATCC) was found in the ND3 gene (positions 1165–1170) of haplotypes L1–L12 and L13, but not in haplotypes L14–L17. An assignment of single haplotypes to a R. lessonae-specific fragment pattern type, however, is not possible. BamHI cuts mtDNA of types B and C into three fragments with sizes of 8,000, 6,400, and 5,600 bp (type B) and 11,500, 8,000 and 470 bp (type C), respectively. Because a BamHI restriction site exists in all the R. lessonae ND3 sequences except for haplotypes L14–L17, these cleavage patterns suggest that one of the restriction sites responsible for the 8,000 bp fragment in types B and C patterns is in the ND3 gene. If so, haplotypes L14–L17 represent neither type B nor C, but an additional, less frequent, R. lessonae-specific haplotype that is probably identical to type LC described by Monnerot et al. (1985) on the basis of the digest profiles of BamHI, EcoRI, and PstI.

Spolsky & Uzzell (1986) also described pattern type D, which is characteristic of Italian water frogs but was also found in R. lessonae and R. esculenta from Switzerland (Hotz et al., 1992) and southern Germany (Ohst & Plötner, unpublished results). The sequence divergence, calculated from shared restriction fragments between the mtDNAs of R. lessonae (B and C) and type D (3.4%), are consistent with distance values obtained on the basis of the ND2+ND3 sequences (3.8–5.0%, mean: 4.4±0.28%).

Tests of neutrality

Tajima’s D-test, which compares the number of segregating sites per site with the nucleotide diversity, revealed no departure from a neutral model of sequence evolution within either R. ridibunda (D=−1.62; 0.10>P>0.05) or R. lessonae (D=0.42; P>0.10). For ND2 and ND3 together, we observed 43 synonymous substitutions and 17 nonsynonymous substitutions within the two species, and 26 amino acid replacements and 156 silent fixed differences between them. The ratios of nonsynonymous to synonymous substitutions within and between species are significantly different from each other (Fisher’s exact test, two tailed: P=0.02, G-test: G=5.64; P=0.02). A NI of 2.37 indicates an excess of amino acid polymorphism, which is consistent with the hypothesis that most segregating functional variants are deleterious and thus underlying purifying selection. If ND2 and ND3 are considered separately, the NI-values are 1.96 and 5.75, respectively. There is, however, no significant departure from neutrality (ND2: Fisher’s exact test, two tailed: P=0.10, G=3.03, P=0.08; ND3: Fisher’s exact test, two tailed: P=0.13, G=2.48; P=0.12).

Geographical distribution of haplotypes

In R. ridibunda, 16 specific haplotypes were found at single localities; only three (R2, R13, R17) were found at two or more localities (Appendix S1, Supplementary Material online). Haplotypes R1–R11 occur in R. ridibunda from the Balkan peninsular (Greece and Albania). Haplotype R13 was found in southern, southeastern, and Central Europe. Haplotype R17 is the most common and characteristic haplotype of Central European R. ridibunda.

Among the R. lessonae-specific haplotypes, three (L1, L2, L17) were found at different localities (Appendix S1, Supplementary Material online). L2, the most common haplotype of R. lessonae, is distributed from southeastern Europe (Danube delta), over Central Europe to Scandinavia. The closely related haplotypes L1 and L3–L12 occur in Central and northern Europe. The less frequent haplotypes L13–L17 were found in northern Italy (L13), in southeastern Europe (L14), and in Central and eastern Europe (L15–L17).

Interspecific genome transfer and haplotype composition in R. esculenta

Of 407 R. ridibunda, 137 (33.7%) possessed mtDNA specific for R. lessonae. The introgressed genomes represent at least six different haplotypes (L1, L2, L8, L10, L12, L15), three of them (L8, L12, L15) observed only in R. ridibunda, two (L1, L10) in both R. ridibunda and R. esculenta, and one (L2) in all three water frog forms. In contrast to R. ridibunda, introgression of R. ridibunda-specific mtDNA has not been detected in R. lessonae.

Of 335 R. esculenta, the majority (90.4%) possessed mtDNA characteristic of R. lessonae or the Italian taxon (R. lessonae sensu lato); haplotypes specific for the Italian R. lessonae stock represented 6.6% of these. Only 9.6% of the R. esculenta contained R. ridibunda-specific mtDNA, represented by haplotypes R13 and (according to ND3 data only) probably R17. All triploid individuals had only R. lessonae-specific mtDNA (Appendix S1, Supplementary Material Online).

R. ridibunda individuals with R. lessonae-specific mtDNA are restricted to Central Europe whereas all individuals examined from southern and eastern Europe (south of 48° N latitude, east of 22° E longitude) possessed exclusively R. ridibunda-specific mtDNA (Fig. 2). This pattern is expressed in a statistically significant relationship between the frequency of individuals with R. lessonae-specific mtDNA (fl) and the geographical coordinates at the 99% confidence level in both R. ridibunda and R. esculenta (Table 3 and Appendix S3, Supplementary Material online). The fitted multiple linear regression models explain 46.3% of the variability in fl for R. ridibunda and 44.5% for R. esculenta. There is a moderately strong negative correlation of fl with longitude under a linear regression model in R. ridibunda (r=−0.55, ANOVA: F-ratio=32.16, d.f.=75, P=0) and in R. esculenta (r=−0.57, ANOVA: F-ratio=33.53, d.f.=71, P=0); and a significant positive correlation between fl and latitude in both forms (R. ridibunda: r=0.56, F-ratio=33.72, d.f.=75, P=0; R. esculenta: r=0.41, F-ratio=13.81, d.f.=71, P=0.0004).

Fig. 2
Geographical distribution of haplotypes found in R. ridibunda and R. esculenta.
Table 3
Results of the multiple regression analysis with the relative frequency of lessonae-specific haplotypes (fl) as the dependent variable and longitude and latitude as independent variables. The Durbin-Watson (DW) statistic tests (Durban & Watson, ...


Patterns of genome transfer

In western Palaearctic water frogs, introgression of R. lessonae mtDNA into R. ridibunda is closely related to the occurrence of the hybridogenetic hybrid R. esculenta. R. ridibunda individuals with introgressed R. lessonae mtDNA are restricted to Central Europe north of 48° N latitude and between 8° and 22° E longitude. In this area, hybridogenetic hybrids mainly live syntopically with a parental species in either lessonae-esculenta (L-E) or ridibunda-esculenta (R-E) populations (reviewed by Plötner, 2005). In eastern Europe (especially Russia and Ukraine), R. ridibunda also co-occurs with hybridogenetic R. esculenta, but in this region, introgression of mitochondrial genomes has not been observed. In contrast to Central European populations, where hybrids are both diploid (LR) and triploid (LLR, RRL), almost all R. esculenta in eastern Europe are diploid (Borkin et al., 1986; Rybacki & Berger, 2001; Borkin et al., 2004, 2006 described exceptions). The observed geographical patterns of mtDNA introgression, in concert with the uniform presence of R. lessonae mtDNA in triploid individuals and the geographical distribution patterns of triploids (Rybacki & Berger, 2001) suggest that the R. lessonae mitochondrial genome was, or is, transferred into R. ridibunda not only by LR females but also, at least in some populations, via triploid females. The occurrence in R. ridibunda of different R. lessonae-specific haplotypes that belong to distinct well-defined clades shows that the genome transfer traces back to several independent transmission events, although it is possible that each of these R. lessonae haplotypes seen in R. ridibunda was introgressed many times independently. Because some of the more divergent haplotypes have not been observed in R. lessonae itself, it is possible that they result from pre-Holocene introgression events.

Four of 38 R. ridibunda (10.5%) examined from the Pannonian Basin possessed R. lessonae-specific mtDNA, which shows that genome introgression takes place despite the scarcity of hybrid males (Berger et al., 1988; Gubányi, 1992; Morozov-Leonov et al., 2003). The unexpectedly high proportion of LR individuals with R. lessonae-specific mtDNA (11 of 23 R. esculenta; 48%) seems, however, to contradict the comparatively low introgression rate observed in Pannonian R. ridibunda. Crosses between diploid hybrid males (LR) and R. lessonae females are a precondition for the formation of LR females with R. lessonae-specific mtDNA that probably act as vehicles for the introgression of R. lessonae mtDNA into R. ridibunda. In contrast to our expectation, such hybrid females have been found, for example, in Hungary (locality 88: one of four individuals) and Slovakia (locality 154 and 156: four of five individuals; Appendix S1, Supplementary Material online). Obviously, crosses between R. ridibunda males and LR females with R. lessonae mtDNA are rare, possibly because of specific mate recognition mechanisms, or do not result in fertile RR progeny.

Presumptive selective forces and mitochondrial-nuclear coadaptation

Whether sequence variation in mtDNA is selectively neutral or under selective constraints is still controversial. In our data set the overall genetic variability in the ND2+ND3 genes as measured by nucleotide substitutions is higher in R. ridibunda, but amino acid substitutions, caused mainly by mutations in the first codon position, are about two times higher in R. lessonae. As indicated by the results of the neutrality tests this difference may reflect purifying selection rather than adaptation, supporting the hypothesis that most segregating functional variants are deleterious and rarely contribute to divergence (e.g., Meiklejohn et al., 2007). Our data do not corroborate the assumption of Bazin et al. (2006) that population size does not influence genetic diversity in mtDNA. The higher genetic variability in R. ridibunda mtDNA is instead consistent with the hypothesis that mtDNA diversity and population size are positively related in animals (e.g., Mulligan et al., 2006). R. ridibunda has a distribution about 0.5 times larger than that of R. lessonae (e.g., Plötner 2005) and based on empirical field data and measured heterozygosity of nuclear loci (Litvinchuk, unpublished), probably has a larger effective population size. This problem needs further investigation, however, particularly because it is possible that the measured genetic variability is influenced by sample biases linked with our concept of species delineation.

Individuals of both R. esculenta and R. ridibunda with R. lessonae mtDNA may have an adaptive advantage rather than disadvantage, especially in their northern ranges, despite possible disruption of nuclear/mitochondrial protein coadaptation in R. ridibunda with introgressed R. lessonae mtDNA. In particular, the O2/CO2 metabolism of introgressed compared to non-introgressed individuals, both as larvae and after metamorphosis, may be significant. Under hypoxic conditions, larvae of R. ridibunda develop less well than larvae of R. lessonae (Plenet et al., 2000a,b). If R. lessonae mitochondria are more effective than R. ridibunda mitochondria under hypoxic conditions, and if this difference depends on enzymes that are encoded in part by the mitochondrial genome, R. ridibunda larvae with R. lessonae-specific mtDNA may be less sensitive to oxygen deficiency caused by hypertrophic conditions or pollution than “normal” R. ridibunda larvae. For metamorphosed R. ridibunda, the type of mitochondrial DNA may also play a role in their survival during hibernation. Because R. ridibunda hibernates exclusively beneath the water surface (Berger, 1982), it is exposed to an oxygen deficiency especially if the waters are covered with an ice sheet for a long period of time. Such condition can lead to a high mortality or even extermination of whole populations (Berger, 1982). Under reduced oxygen pressure, the survival time of R. lessonae metamorphs was higher than those of R. ridibunda metamorphs (Tunner & Nopp, 1979; Lutschinger, 1988), which also demonstrates that R. ridibunda is more sensitive to oxygen deficiency than R. lessonae.

The large divergence between both the nuclear and the mt genomes of R. lessonae and R. ridibunda (the modified Nei distances calculated on the basis of 31 protein coding loci varied between 0.39 and 0.60; Beerli et al., 1996) suggests that intergenomic coadaptation would be strongly disrupted when R. lessonae mtDNAs are introgressed into R. ridibunda. Because more than 90% of mitochondrial proteins are encoded in the nucleus, intergenomic protein coadaptation is assumed to be directly correlated with fitness (e.g., Burton et al., 2006; Rand et al., 2006; Gusdon et al., 2007). The extent of transfer of R. lessonae mtDNA into R. ridibunda (33.7%) suggests that the presence of R. lessonae mtDNA does not significantly influence mitochondrial–nuclear interaction and hence, the fitness of this species, despite large divergence between the mitochondrial genomes of R. lessonae and R. ridibunda. It is possible, of course, that disruption of nuclear/mitochondrial protein coadaptation in water frogs with introgressed mtDNA is variable in its significance. The nature of this nuclear-mtDNA epistasis is not clear, however.

Although the possibility that introgressed R. lessonae mtDNA genomes provide an adaptive advantage to northern R. ridibunda (and perhaps also to northern R. esculenta) is a tempting hypothesis to be tested, there is, in clonal mole salamanders of the genus Ambystoma, a striking case of “introgressed” mitochondrial genomes in which the phylogenetic divergence between the nuclear genomes of the normal host species and the phylogenetically closest possible source of the mitochondrial sequences is enormous. Here, an almost invariant mitochondrial genome (Spolsky et al., 1992; Spolsky & Uzzell, unpublished) exists independently for perhaps 5Õ105 years (Spolsky et al., 1992; Hedges et al., 1992) within the widespread triploid taxa Ambystoma platineum and A. tremblayi (Uzzell, 1964) of the subgenus Ambystoma, although the mitochondrial sequence itself apparently is derived from A. barbouri (Robertson et al., 2006) of the distantly related subgenus Linguaelapsus. If strong coadaptation between proteins encoded by the nuclear and mitochondrial genomes of a taxon or population is a general rule and the disruption of this coadaptation by hybridization a frequent source of postzygotic incompatibility between taxa (Burton et al., 2006), the mitochondrial genomes of clonal Ambystoma must possess remarkable properties. What these might be for clonal Ambystoma is completely unknown, but at least for the frogs a plausible though not yet tested hypothesis can be proposed.

Biogeographic implications

Provided that the mt ND2 and ND3 genes of western Palearctic water frogs diverge at about 2.0–2.5% per Myr (estimated from the sequence divergence in these two genes between R. cretensis and Anatolian water frogs, which are thought to have been separated for about 5 My, Beerli et al. 1996), the divergence between different haplotypes within both R. ridibunda (maximum distance value 1.6%) and R. lessonae (maximum distance value 0.7%) indicates that much of the early intraspecific differentiation took place prior to the most recent glacial cycle. During the Würm glaciation, presumptive refuges are the southern Balkans (especially the Peloponnisos and adjacent areas) for R. ridibunda and the western (and probably northern) Black Sea region (for both R. ridibunda and R. lessonae). Haplotypes R1-R9 (the Greek ridibunda lineage sensu stricto) remained more or less confined to their potential refuge and nearby regions, resulting in a much smaller current range than other haplotypes (for example R13 and R17), which probably underwent rapid northward expansion with the advent of Holocene warming. Similar scenarios have been suggested for the European pond turtle, Emys orbicularis (Lenk et al., 1999), the newt species Triturus vulgaris (Babik et al., 2005), the fire-bellied toad Bombina bombina (Hofmann et al., 2007) and the grasshopper Chorthippus parallelus (Hewitt, 2001).

Because of climatic oscillations, the post-Würm colonization of Central Europe probably involved a sequence of range expansions followed by contractions and attendant reductions in population sizes (bottlenecks) that may have reduced genetic diversity in the northern populations. Such reductions might be lower in cold-tolerant taxa (Taberlet et al., 1998, Hewitt, 2004). This hypothesis is supported by the immunological (Uzzell, 1979, 1983),mitochondrial, and ecological data obtained in Central and northern European water frog populations. Based on a comparison of the distribution ranges (R. lessonae goes farther north than R. ridibunda), the altitude distribution of water frog species (R. lessonae populates habitats up to 1,000 m above sea level while R. ridibunda is restricted to the lowlands; Günther, 1990, 1997a,b), and the results of preliminary ecological experiments that showed a higher hatching success of R. lessonae larvae at temperatures below 13 °C (Günther, 1974), it seems probable that R. ridibunda is a rather cold sensitive species that recolonized the deglaciated northern parts of Europe later than R. lessonae. The amount of genetic variability observed in the mtDNA of R. lessonae (15 haplotypes) and R. ridibunda (only two haplotypes) from Central and northern Europe corresponds well with this assumption.

Supplementary Material


Supplementary Material The following supplementary material is available for this article online:

Appendix S1. Origin, phenotype, and number of individuals investigated, method applied for mtDNA typing and haplotypes detected.


Appendix S2. Protocols used for amplification and sequencing of the mtDNA.


Appendix S3. Component effects plot of latitude and longitude on the relative frequency of individuals with R. lessonae-specific mtDNA (fl) for R. ridibunda and R. esculenta.


We thank Bradley Anholt and two anonymous reviewers for constructive and critical comments on the manuscript. For providing water frog samples, we thank Martina Arioli and Christian Jakob (Zürich), Rainer Günther (Berlin), Petr Kotlik (Libechov), Peter Mikuliek (Prague), Andreas Nöllert (Jena), Ulrich Scheidt (Erfurt), and Dirk Schmeller (Leipzig). Tissue samples from Greece were made available by the Greek Ministry of Rural Development and Food, kindly mediated by Doris Tippmann (Embassy of the FRG, Athens). Grateful thanks are given to Robert Schreiber, Kai-Joachim Schultze, and Ronny Knop (Museum für Naturkunde, Berlin), and Alexandra Schulmeister (University Bergen, Norway) for technical assistance. This research was supported by the Deutsche Forschungsgemeinschaft (grants PL 213/3-1, 3-2, 3-3) and the Swiss National Fund (grants 31-37579.93, 31-59144.99, 31-103903/1, and 31-64004.00). Peter Beerli was partly supported by the joint NSF/NIGMS Mathematical Biology program under NIH grant R01 GM 078985. Spartak N. Litvinchuk was partly supported by a DAAD grant under A/03/06782.


  • Abe TA, Spence JR, Sperling FAH. Mitochondrial introgression is restricted relative to nuclear markers in a water strider (Hemiptera: Gerridae) hybrid zone. Can. J. Zoolog. 2005;83:432–444.
  • Babik W, Branicki W, Crnobrnja-Isailović J, Cogălniceanu D, Sas I, Olgun K, Poyarkov HA, Garcia-París M, Arntzen JW. Phylogeography of two European newt species – discordance between mtDNA and morphology. Mol. Ecol. 2005;14:2475–2491. [PubMed]
  • Bachtrog D, Thornton K, Clark A, Andolfatto P. Extensive introgression of mitochondrial DNA relative to nuclear genes in the Drosophila yakuba species group. Evolution. 2006;60:292–302. [PubMed]
  • Ballard JWO, Whitlock MC. The incomplete natural history of mitochondria. Mol. Ecol. 2004;13:729–744. [PubMed]
  • Bazin E, Glémin S, Galtier N. Population size does not influence mitochondrial genetic diversity in animals. Science. 2006;312:570–572. [PubMed]
  • Beerli P, Hotz H, Uzzell T. Geologically dated sea barriers calibrate a protein clock for Aegean water frogs. Evolution. 1996;50:1676–1687.
  • Berger L. Some characteristics of the crosses within Rana esculenta complex in postlarval development. Ann. Zool. 1970;27:373–416.
  • Berger L. Hibernation of the European water frogs. Zoologica Poloniae. 1982;29:187–202.
  • Berger L, Günther R. Genetic composition and reproduction of water frog populations (Rana kl. esculenta Synklepton) near nature reserve Serrahn, GDR. Archiv Naturschutz u. Landschaftsf., Berlin. 1988;28:265–280.
  • Berger L, Uzzell T, Hotz H. Sex determination and sex ratios in western Palearctic water frogs: XX and XY female hybrids in the Pannonian Basin? Proc. Acad. Nat. Sci. Phila. 1988;140:220–239.
  • Berger L, Uzzell T, Hotz H. Postzygotic reproductive isolation between Mendelian species of European water frogs. Zoologica Poloniae. 1994;39:209–242.
  • Bergthorsson U, Adams KL, Thomason B, Palmer JD. Widespread horizontal transfer of mitochondrial genes in flowering plants. Nature. 2003;424:197–201. [PubMed]
  • Blankenhorn HJ. Reproduction and mating behavior in Rana lessonae-Rana esculenta mixed populations. In: Taylor DH, Guttman SI, editors. The Reproductive Biology of Amphibians. New York & London: Plenum Press; 1977. pp. 389–410.
  • Borkin LJ, Garanin WI, Tichenko NT, Zaune IA. Some results in the green frogs survey in the USSR. Mitt. Zool. Mus. Berlin. 1979;55:153–170.
  • Borkin LJ, Zaune IA, Pikulik MM, Sokolova TM. Distribution and structure of green frog complex in the USSR. In: Roček Z, editor. Studies in Herpetology. 1986. pp. 675–678. Prague.
  • Borkin LJ, Lada GA, Litvinchuk SN, Melnikov DA, Rosanov JM. The first record of mass triploidy in hybridogenic green frog Rana esculenta in Russia (Rostov Oblast') Russ. J. Herpetology. 2006;13:77–82.
  • Borkin LJ, Korshunov AV, Lada GA, Litvinchuk SN, Rosanov JM, Shabanov DA, Zinenko AI. Mass occurrence of polyploid green frogs (Rana esculenta complex) in eastern Ukraine. Russ. J. Herpetology. 2004;11:194–213.
  • Bucci S, Raggianti M, Mancino G, Berger L, Hotz H, Uzzell T. Lampbrush and mitotic chromosomes of the hemiclonally reproducing hybrid Rana esculenta and its parental species. J. Exp. Zool. 1990;255:37–56. [PubMed]
  • Burton RS, Ellison CK, Harrison JS. The sorry state of F2 hybrids: consequences of rapid mitochondrial DNA evolution in allopatric populations. Am. Nat. 2006;168:S14–S24. [PubMed]
  • Croucher PJP, Oxford GS, Searle JB. Mitochondrial differentiation, introgression and phylogeny of species in the Tegenaria atrica group (Araneae: Agelenidae) Biol. J. Linn. Soc. 2004;81:79–89.
  • Dabrowski A, Fraser R, Confer JL, Lovette IJ. Geographic variability in mitochondrial introgression among hybridizing populations of Golden-winged (Vermivora chrysoptera) and Blue-winged (V. pinus) warblers. Conserv. Genet. 2005;6:843–853.
  • Dubois A, Ohler A. Frogs of the subgenus Pelophylax (Amphibia, Anura, Genus Rana): a catalogue of available scientific names, with comments on name-bearing types, complete synonymies, proposed common names, and maps showing all type localities. Zoologica Poloniae. 1994;39:139–204.
  • Durbin J, Watson GS. Testing for serial correlation in least squares regression. I. Biometrika. 1950;37:409–428. [PubMed]
  • Durbin J, Watson GS. Testing for serial correlation in least squares regression. 2. Biometrika. 1951;38:159–177. [PubMed]
  • Durbin J, Watson GS. Testing for serial correlation in least squares regression. 3. Biometrika. 1971;58:1–19.
  • Fredsted T, Wincentz T, Villesen P. Introgression of mountain hare (Lepus timidus) mitochondrial DNA into brown hares (Lepus europaeus) in Denmark. BMC Ecology. 2006;6:17. online publication, http://www.biomedcentral.com/1472-6785/6/17. [PMC free article] [PubMed]
  • Graf J-D, Polls Pelaz M. Evolutionary genetics of the Rana esculenta complex. In: Dawley RM, Bogart JP, editors. Evolution and Ecology of Unisexual Vertebrates. New York State Museum; 1989. pp. 289–301. Bulletin 466.
  • Grossenbacher K. Verbreitung der Wasserfrösche in der Schweiz. In: Günther R, Klewen R, editors. Beiträge zur Biologie und Bibliographie (1960–1987) der europäischen Wasserfrösche. Duisburg: Verlag für Ökologie u. Faunistik; 1988. pp. 129–134. (Fre). Jb. Feldherp., Beiheft 1.
  • Gubányi A. Distribution of green frogs (Rana esculenta complex, Anura: Ranidae) in Hungary. In: Korsós Z, Kiss I, editors. Proc. Sixth Ord. Gen. Meet. S. E. H. Budapest, Hungary: 1992. pp. 205–210.
  • Günther R. Neue Daten zur Verbreitung und Ökologie der Grünfrösche (Anura, Ranidae) in der DDR. Mitt. Zool. Mus. Berlin. 1974;50:287–298.
  • Günther R. Zum natürlichen Vorkommen und zur Morphologie triploider Teichfrösche, "Rana esculenta", L., in der DDR (Anura, Ranidae) Mitt. Zool. Mus. Berlin. 1975;51:145–158.
  • Günther R. Die Erythrozytengröβe als Kriterium zur Unterscheidung diploider und triploider Teichfrösche, Rana ,,esculenta“ L. (Anura) Biol. Zentralblatt. 1977;96:457–466.
  • Günther R. Zur Populationsgenetik der mitteleuropäischen Wasserfrösche des Rana esculenta-Synkleptons (Anura, Ranidae) Zool. Anz. Jena. 1983;211:43–54.
  • Günther R. Die Wasserfrösche Europas. A. Ziemsen, Wittenberg-Lutherstadt; 1990. Die Neue Brehm-Bücherei 600.
  • Günther R. Rana lessonae Camerano, 1882. In: Gasc JP, Cabela A, Crnobrnja-Isailovic J, et al., editors. Atlas of Amphibians and Reptiles in Europe. Paris: Societas Europaea Herpetologica, Muséum National d’Histoire Naturelle; 1997a. pp. 148–149. (Fre).
  • Günther R. Rana ridibunda Pallas, 1771. In: Gasc JP, Cabela A, Crnobrnja-Isailovic J, et al., editors. Atlas of Amphibians and Reptiles in Europe. Paris: Societas Europaea Herpetologica, Muséum National d’Histoire Naturelle; 1997b. pp. 154–155. (Fre).
  • Günther R, Plötner J. Zur Problematik der klonalen Vererbung bei Rana kl. esculenta (Anura) In: Günther R, Klewen R, editors. Beiträge zur Biologie und Bibliographie (1960–1987) der europäischen Wasserfrösche. Duisburg: Verlag für Ökologie u. Faunistik; 1988. pp. 23–46. (Fre). Jb. Feldherp., Beiheft 1.
  • Günther R, Plötner J, Tetzlaff I. Zu einigen Merkmalen der Wasserfrösche (Rana synkl. esculenta) des Donau-Deltas. Salamandra. 1991;27:246–265.
  • Günther R, Uzzell T, Berger L. Inheritance patterns in triploid Rana ,,esculenta“ (Amphibia, Salientia) Mitt. Zool. Mus. Berlin. 1979;55:35–57.
  • Gusdon AM, Votyakova TV, Reynolds IJ, Mathews CE. Nuclear and mitochondrial interaction involving mt-ND2 leads to increased mitochondrial reactive oxygen species production. J. Biol. Chemistry. 2007;282:5171–5179. [PubMed]
  • Hedges SB, Bogart JP, Maxson LR. Ancestry of unisexual salamanders. Nature. 1992;356:708–710. [PubMed]
  • Hewitt GM. Speciation, hybrid zones and phylogeography - or seeing genes in space and time. Mol. Ecol. 2001;10:537–549. [PubMed]
  • Hewitt GM. Genetic consequences of climatic oscillations in the Quaternary. Phil. Trans. Roy. Soc. London B. 2004;359:83–195. [PMC free article] [PubMed]
  • Higgins DG, Bleasby AJ, Fuchs R. CLUSTAL V: improved software for multiple sequence alignment. CABIOS. 1992;8:189–191. [PubMed]
  • Hofmann S, Spolsky C, Uzzell T, Cogǎlniceanu D, Babik W, Szymura JM. Phylogeography of the fire-bellied toads Bombina: independent Pleistocene histories inferred from mitochondrial genomes. Mol. Ecol. 2007;16:2301–2316. [PubMed]
  • Hotz H, Mancino G, Bucci-Innocenti S, Raggianti M, Berger L, Uzzell T. Rana ridibunda varies geographically in inducing clonal gametogenesis in interspecies hybrids. J. Exp. Zool. 1985;236:199–210.
  • Hotz H, Beerli P, Spolsky C. Mitochondrial DNA reveals formation of nonhybrid frogs by natural matings between hemiclonal hybrids. Mol. Biol. Evol. 1992;9:610–620. [PubMed]
  • Huelsenbeck JP, Rannala B. Frequentist properties of Bayesian posterior probabilities of phylogenetic trees under simple and complex substitution models. Syst. Biol. 2004;53:904–913. [PubMed]
  • Huelsenbeck JP, Ronquist F. Bayesian analysis of molecular evolution using MrBayes. In: Nielsen R, editor. Statistical Methods in Molecular Evolution. New York: Springer; 2005. pp. 183–232.
  • Kocher TD, Thomas WK, Mayer A, Edwards SV, Pääbo S, Villablanca FX, Wilson AC. Dynamics of mitochondrial evolution in animals: amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. USA. 1989;86:6196–6200. [PMC free article] [PubMed]
  • Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief. Bioinform. 2004;5:150–163. [PubMed]
  • Lengagne T, Grolet O, Joly P. Male mating speed promote hybridization in the Rana lessonae-Rana esculenta waterfrog system. Behav. Ecol. Sociobiol. 2006;60:123–130.
  • Lenk P, Fritz U, Joger U, Wink M. Mitochondrial phylogeography of the European pond turtle, Emys orbicularis (Linnaeus 1758) Mol. Ecol. 1999;8:1911–1922. [PubMed]
  • Lutschinger G. Zur Überwinterung der Wasserfrösche in den Donau- und Marchauen. In: Günther R, Klewen R, editors. Beiträge zur Biologie und Bibliographie (1960–1987) der europäischen Wasserfrösche. Duisburg: Verlag für Ökologie u. Faunistik; 1988. pp. 153–160. (Fre). Jb. Feldherp. Beiheft 1.
  • McDonald JHM, Kreitman M. Adaptive protein evolution at the Adh locus in Drosophila. Nature. 1991;351:652–654. [PubMed]
  • Meyer A. Evolution of mitochondrial DNA in fishes. In: Hochachka PW, Mommsen TP, editors. Biochemistry and Molecular Biology of Fishes. Vol. 2. Amsterdam: Elsevier Science Publishers; 1993. pp. 1–38.
  • Meiklejohn CD, Montooth KL, Rand DM. Positive and negative selection on the mitochondrial genome. Trends Genet. 2007;23:259–263. [PubMed]
  • Monnerot M, Dubois A, Tunner H. Mitochondrial DNA polymorphism among Rana ridibunda, Rana lessonae and Rana kl. esculenta: preliminary study. Alytes. 1985;4:101–112.
  • Morozov-Leonov SY, Mezhzherin SV, Kurtyak FF. The Genetic structure of unisexual hybrid populations of frogs Rana esculenta complex from the Transcarpathian lowlands. Cytol. Genet. 2003;37:43–47. [PubMed]
  • Mulligan CJ, Kitchen A, Miyamoto MM. Comment on “Population size does not influence mitochondrial genetic diversity in animals” Science. 2006;314:1390a. [PubMed]
  • Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 1979;76:5269–5273. [PMC free article] [PubMed]
  • Nylander JAA, Wilgenbusch JC, Warren DL, Swofford DL. AWTY (Are We There Yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics. 2007 Advance Access ( http://creativecommons.org/licenses/by-nc/2.0/uk/) [PubMed]
  • Pagano A, Dubois A, Lesbarrères D, Lodé T. Frog alien species: a way for genetic invasion. C. R. Biol. 2003;326:85–92. [PubMed]
  • Plenet S, Hervant F, Joly P. Ecology of the hybridogenetic Rana esculenta complex: differential oxygen requirements of tadpoles. Evol. Ecol. 2000;14:13–23.
  • Plenet S, Pagano A, Joly P, Fouillet P. Variation of plastic responses to oxygen availability within the hybridogenetic Rana esculenta complex. J. Evol. Biol. 2000;13:20–28.
  • Plötner J. Von Märtyrern der Wissenschaft zur biologischen Sensation. Laurenti Verlag, Bielefeld; 2005. Die westpaläarktischen Wasserfrösche.
  • Plötner J, Ohst T. New hypotheses on the systematics of the western Palearctic water frog complex (Anura, Ranidae) Mitt. Mus. Nat.kd. Berl., Zool. Reihe. 2001;77:5–21.
  • Rand DM, Kann LM. Excess amino acid polymorphism in mitochondrial DNA: contrasts among genes from Drosophila, mice, and humans. Mol. Biol. Evol. 1996;13:735–748. [PubMed]
  • Rand DM, Fry A, Sheldahl L. Nuclear-mitochondrial epistasis and Drosophila aging: introgression of Drosophila simulans mtDNA modifies longevity in D. melanogaster nuclear backgrounds. Genetics. 2006;172:329–341. [PMC free article] [PubMed]
  • Reichert AS, Neupert W. Mitochondriomics or what makes us breathe. Trends Genet. 2004;20:555–562. [PubMed]
  • Ronquist F, Huelsenbeck JP. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003;19:1572–1574. [PubMed]
  • Robertson AV, Ramsden C, Niedzwiecki J, Fu J, Bogart JP. An unexpected recent ancestor of unisexual Ambystoma. Mol. Ecol. 2006;15:3339–3351. [PubMed]
  • Rozas J, Sánches JC, DelBarrio X, Rozas R. DnaSP, DNA polymorphism analysis by the coalescent and other methods. Bioinformatics. 2003;19:2496–2497. [PubMed]
  • Ruedi M, Smith MF, Patton JL. Phylogenetic evidence of mitochondrial DNA introgression among pocket gophers in New Mexico (family Geomyidae) Mol. Ecol. 1997;6:453–462. [PubMed]
  • Rybacki M, Berger L. Types of water frog populations (Rana esculenta complex) in Poland. Mitt. Mus. Nat.kd. Berl., Zool. Reihe. 2001;77:51–57.
  • Schultz RJ. Hybridization, unisexuality and polyploidy in the teleost Poeciliopsis (Poecilidae) and other vertebrates. Am. Nat. 1969;103:605–619.
  • Spolsky C, Uzzell T. Natural interspecies transfer of mitochondrial DNA in amphibians. Proc. Natl. Acad. Sci. USA. 1984;81:5802–5805. [PMC free article] [PubMed]
  • Spolsky C, Uzzell T. Evolutionary history of the hybridogenetic hybrid frog Rana esculenta as deduced from mtDNA analyses. Mol. Biol. Evol. 1986;3:44–56. [PubMed]
  • Spolsky CM, Phillips CA, Uzzell T. Antiquity of clonal salamander lineages revealed by mitochondrial DNA. Nature. 1992;356:706–708. [PubMed]
  • Sullivan JP, Lavoué S, Arnegard ME, Hopkins CD. AFLPs resolve phylogeny and reveal mitochondrial introgression within a species flock of African electric fish (Mormyroidea: Teleostei) Evolution. 2004;58:825–841. [PubMed]
  • Sumida M, Kanamori Y, Kaneda H, Kato Y, Nishioka M, Hasegawa M, Yonekawa H. Complete nucleotide sequence and gene rearrangement of the mitochondrial genome of the Japanese pond frog Rana nigromaculata. Genes Genet. Syst. 2001;76:311–325. [PubMed]
  • Swofford DL. Phylogenetic Analysis Using Parsimony (*and other Methods). Version 4. Sunderland, Massachusetts: Sinauer Associates; 2003. PAUP*.
  • Taberlet P, Fumagalli L, Wust-Saucy A-G, Cosson J-F. Comparative phylogeography and postglacial colonization routes in Europe. Mol. Ecol. 1998;7:453–464. [PubMed]
  • Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics. 1989;123:585–595. [PMC free article] [PubMed]
  • Tunner H-G, Nopp H. Heterosis in the common European water frog. Naturwissenschaften, Berlin. 1979;66:268–269. [PubMed]
  • Upholt WB. Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res. 1977;4:1257–1265. [PMC free article] [PubMed]
  • Uzzell T. Relations of the diploid and triploid species of the Ambystoma jeffersonianum complex (Amphibia, Caudata) Copeia. 1964;1964:257–300.
  • Uzzell T. Immunological variation in Rana lessonae (Amphibia, Salientia) Mitt. Zool. Mus. Berlin. 1979;55:29–33.
  • Uzzell T. An immunological survey of Italian water frogs (Salientia Ranidae) Herpetologica. 1983;39:225–234.
  • Uzzell T, Berger L. Electrophoretic phenotypes of Rana ridibunda, Rana lessonae, and their hybridogenetic associate, Rana esculenta. Proc. Acad. Nat. Sci. Phila. 1975;127:13–24.
  • Uzzell T, Günther R, Berger L. Rana ridibunda and Rana esculenta: a leaky hybridogenetic system (Amphibia Salientia) Proc. Acad. Nat. Sci. Phila. 1977;128:147–171.
  • Vinogradov AE, Borkin LJ, Günther R, Rosanov JM. Genome elimination in diploid and triploid Rana esculenta males: cytological evidence from DNA flow cytometry. Genome. 1991;33:619–627. [PubMed]
  • Wood FS. The use of individual effects and residuals in fitting equation to data. Technometrics. 1973;15:677–695.
  • Yamada M, Higuchi M, Goto A. Extensive introgression of mitochondrial DNA found between two genetically divergent forms of threespine stickleback, Gasterosteus aculeatus, around Japan. Environ. Biol. Fishes. 2001;61:269–284.
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