• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Jan 1990; 64(1): 401–405.
PMCID: PMC249115

Construction of a recombinant bovine leukemia virus vector for analysis of virus infectivity.


A recombinant bovine leukemia virus (BLV) was constructed in which the X region was replaced with the bacterial neomycin resistance gene controlled by the simian virus 40 early promoter. This virus, termed BLV-SVNEO, is a self-packaging, activator-dependent retroviral vector. Introduction of the plasmid pBLV-SVNEO into mammalian cells resulted in constitutive expression of the neo gene, whereas the BLV structural genes, gag, pol, and env, were expressed only in the presence of the two regulatory proteins, Tax and Rex. The production and release of recombinant virus by cells transfected with pBLV-SVNEO were proportional to the number of G418-resistant colonies that developed after susceptible cells were exposed to the filtered culture medium. BLV-SVNEO was able to infect cell lines of human, bovine, canine, feline, and murine origin. BLV-producing cell lines were resistant to superinfection with BLV-SVNEO. This cell-virus system should facilitate molecular genetic studies of BLV and will provide a rapid, quantitative measure of BLV infectivity in a variety of cell types. These studies also demonstrate the feasibility of using activator-dependent retroviral vectors such as BLV-SVNEO to deliver foreign genes into cells and eventually animals.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1002K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Derse D. Bovine leukemia virus transcription is controlled by a virus-encoded trans-acting factor and by cis-acting response elements. J Virol. 1987 Aug;61(8):2462–2471. [PMC free article] [PubMed]
  • Derse D. trans-acting regulation of bovine leukemia virus mRNA processing. J Virol. 1988 Apr;62(4):1115–1119. [PMC free article] [PubMed]
  • Djilali S, Parodi AL, Levy D. Bovine leukemia virus replicates in sheep B lymphocytes under a T cell released factor. Eur J Cancer Clin Oncol. 1987 Jan;23(1):81–85. [PubMed]
  • Djilali S, Parodi AL, Levy D, Cockerell GL. Development of leukemia and lymphosarcoma induced by bovine leukemia virus in sheep: a hematopathological study. Leukemia. 1987 Nov;1(11):777–781. [PubMed]
  • Ghysdael J, Bruck C, Kettmann R, Burny A. Bovine leukemia virus. Curr Top Microbiol Immunol. 1984;112:1–19. [PubMed]
  • Graves DC, Ferrer JF. In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. Cancer Res. 1976 Nov;36(11 Pt 1):4152–4159. [PubMed]
  • Hoss HE, Olson C. Infectivity of bovine C- type (leukemia) virus for sheep and goats. Am J Vet Res. 1974 May;35(5):633–637. [PubMed]
  • Itohara S, Sekikawa K. Molecular cloning of infectious proviral genomes of bovine leukemia virus. Virology. 1987 Jul;159(1):158–160. [PubMed]
  • Katoh I, Yoshinaka Y, Sagata N, Ikawa Y. The bovine leukemia virus X region encodes a trans-activator of its long terminal repeat. Jpn J Cancer Res. 1987 Feb;78(2):93–98. [PubMed]
  • Kettmann R, Deschamps J, Cleuter Y, Couez D, Burny A, Marbaix G. Leukemogenesis by bovine leukemia virus: proviral DNA integration and lack of RNA expression of viral long terminal repeat and 3' proximate cellular sequences. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2465–2469. [PMC free article] [PubMed]
  • Kettmann R, Marbaix G, Cleuter Y, Portetelle D, Mammerickx M, Burny A. Genomic integration of bovine leukemia provirus and lack of viral RNA expression in the target cells of cattle with different responses to BLV infection. Leuk Res. 1980;4(6):509–519. [PubMed]
  • Lévy D, Kettmann R, Marchand P, Djilali S, Parodi AL. Selective tropism of bovine leukemia virus (BLV) for surface immunoglobulin-bearing ovine B lymphocytes. Leukemia. 1987 May;1(5):463–465. [PubMed]
  • Mamoun RZ, Astier-Gin T, Kettmann R, Deschamps J, Rebeyrotte N, Guillemain BJ. The pX region of the bovine leukemia virus is transcribed as a 2.1-kilobase mRNA. J Virol. 1985 May;54(2):625–629. [PMC free article] [PubMed]
  • Nötzel U, Drescher B, Rosenthal S. Detection of bovine leukaemia virus RNA sequences in non-cultivated peripheral lymphocytes by in situ hybridization with 3H-labelled viral cDNA. Acta Virol. 1982 Jan;26(1-2):33–40. [PubMed]
  • Paul PS, Pomeroy KA, Johnson DW, Muscoplat CC, Handwerger BS, Soper FF, Sorensen DK. Evidence for the replication of bovine leukemia virus in the B lymphocytes. Am J Vet Res. 1977 Jun;38(6):873–876. [PubMed]
  • Rice NR, Simek SL, Dubois GC, Showalter SD, Gilden RV, Stephens RM. Expression of the bovine leukemia virus X region in virus-infected cells. J Virol. 1987 May;61(5):1577–1585. [PMC free article] [PubMed]
  • Rosen CA, Sodroski JG, Willems L, Kettmann R, Campbell K, Zaya R, Burny A, Haseltine WA. The 3' region of bovine leukemia virus genome encodes a trans-activator protein. EMBO J. 1986 Oct;5(10):2585–2589. [PMC free article] [PubMed]
  • Sagata N, Tsuzuku-Kawamura J, Nagayoshi-Aida M, Shimizu F, Imagawa K, Ikawa Y. Identification and some biochemical properties of the major XBL gene product of bovine leukemia virus. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7879–7883. [PMC free article] [PubMed]
  • Sagata N, Yasunaga T, Ikawa Y. Two distinct polypeptides may be translated from a single spliced mRNA of the X genes of human T-cell leukemia and bovine leukemia viruses. FEBS Lett. 1985 Nov 11;192(1):37–42. [PubMed]
  • Sommerfelt MA, Williams BP, Clapham PR, Solomon E, Goodfellow PN, Weiss RA. Human T cell leukemia viruses use a receptor determined by human chromosome 17. Science. 1988 Dec 16;242(4885):1557–1559. [PubMed]
  • Van den Broeke A, Cleuter Y, Chen G, Portetelle D, Mammerickx M, Zagury D, Fouchard M, Coulombel L, Kettmann R, Burny A. Even transcriptionally competent proviruses are silent in bovine leukemia virus-induced sheep tumor cells. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9263–9267. [PMC free article] [PubMed]
  • Willems L, Gegonne A, Chen G, Burny A, Kettmann R, Ghysdael J. The bovine leukemia virus p34 is a transactivator protein. EMBO J. 1987 Nov;6(11):3385–3389. [PMC free article] [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...