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J Bacteriol. Jun 1971; 106(3): 876–881.
PMCID: PMC248714

Fatty Acid Composition of Thermophilic, Mesophilic, and Psychrophilic Clostridia

Abstract

The fatty acid composition of two thermophilic anaerobes was determined, and the results were compared with those from a mesophilic and a psychrophilic anaerobe. Notable differences were that the thermophiles contained a higher content of saturated straight- and branched-chain fatty acids, and, of the latter, iso C15 was the predominant type. The mesophile and psychrophile were characterized by having a higher percentage of unsaturated fatty acids. An unidentified fatty acid, present in all of the organisms, was purified from the psychrophile. By physical and chemical analysis the structure of the unknown acid was resolved and found to be the unsaturated cyclopropane fatty acid, 12,13-methylene-9-tetradecenoic acid.

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Selected References

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  • Archibald FM, Skipski VP. Determination of fatty acid content and composition in ultramicro lipid samples by gas-liquid chromatography. J Lipid Res. 1966 May;7(3):442–445. [PubMed]
  • Brian BL, Gardner EW. A simple procedure for detecting the presence of cyclopropane fatty acids in bacterial lipids. Appl Microbiol. 1968 Apr;16(4):549–552. [PMC free article] [PubMed]
  • CARNAHAN JE, CASTLE JE. Some requirements of biological nitrogen fixation. J Bacteriol. 1958 Feb;75(2):121–124. [PMC free article] [PubMed]
  • Christie WW, Holman RT. Mass spectrometry of lipids. I. Cyclopropane fatty acid esters. Lipids. 1966 May;1(3):176–182. [PubMed]
  • Daron HH. Fatty acid composition of lipid extracts of a thermophilic Bacillus species. J Bacteriol. 1970 Jan;101(1):145–151. [PMC free article] [PubMed]
  • FOLCH J, LEES M, SLOANE STANLEY GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed]
  • GOLDFINE H, BLOCH K. On the origin of unsaturated fatty acids in clostridia. J Biol Chem. 1961 Oct;236:2596–2601. [PubMed]
  • JAMES AT. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. Methods Biochem Anal. 1960;8:1–59. [PubMed]
  • McCloskey JA, Law JH. Ring location in cyclopropane fatty acid esters by a mass spectrometric method. Lipids. 1967 May;2(3):225–230. [PubMed]
  • MERCER WA, VAUGHN RH. The characteristics of some thermophilic, tartrate-fermenting anaerobes. J Bacteriol. 1951 Jul;62(1):27–37. [PMC free article] [PubMed]
  • SCHEURBRANDT G, BLOCH K. Unsaturated fatty acids in microorganisms. J Biol Chem. 1962 Jul;237:2064–2068. [PubMed]
  • Shen PY, Coles E, Foote JL, Stenesh J. Fatty acid distribution in mesophilic and thermophilic strains of the genus Bacillus. J Bacteriol. 1970 Aug;103(2):479–481. [PMC free article] [PubMed]
  • SINCLAIR NA, STOKES JL. ISOLATION OF OBLIGATELY ANAEROBIC PSYCHROPHILIC BACTERIA. J Bacteriol. 1964 Mar;87:562–565. [PMC free article] [PubMed]
  • WILDER M, VALENTINE RC, AKAGI JM. FERREDOXIN OF CLOSTRIDIUM THERMOSACCHAROLYTICUM. J Bacteriol. 1963 Oct;86:861–865. [PMC free article] [PubMed]
  • Yao M, Walker HW, Lillard DA. Fatty acids from vegetative cells and spores of Bacillus stearothermophilus. J Bacteriol. 1970 Jun;102(3):877–878. [PMC free article] [PubMed]

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