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J Virol. Nov 1990; 64(11): 5669–5673.
PMCID: PMC248627

Determination of influenza virus proteins required for genome replication.

Abstract

An artificial vaccinia virus vector-driven replication system for influenza virus RNA has been developed. In this system, a synthetic NS-like gene is replicated and expressed by influenza virus proteins supplied through infection with vaccinia virus recombinant vectors. The minimum subset of influenza virus proteins needed for specific replication and expression of the viral ribonucleoprotein was found to be the three polymerase proteins (PB1, PB2, and PA) and the nucleoprotein.

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Selected References

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  • Beaton AR, Krug RM. Synthesis of the templates for influenza virion RNA replication in vitro. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4682–4686. [PMC free article] [PubMed]
  • Beaton AR, Krug RM. Transcription antitermination during influenza viral template RNA synthesis requires the nucleocapsid protein and the absence of a 5' capped end. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6282–6286. [PMC free article] [PubMed]
  • Blaas D, Patzelt E, Kuechler E. Cap-recognizing protein of influenza virus. Virology. 1982 Jan 15;116(1):339–348. [PubMed]
  • Blaas D, Patzelt E, Kuechler E. Identification of the cap binding protein of influenza virus. Nucleic Acids Res. 1982 Aug 11;10(15):4803–4812. [PMC free article] [PubMed]
  • Buonagurio DA, Krystal M, Palese P, DeBorde DC, Maassab HF. Analysis of an influenza A virus mutant with a deletion in the NS segment. J Virol. 1984 Feb;49(2):418–425. [PMC free article] [PubMed]
  • Cooper JA, Moss B. In vitro translation of immediate early, early, and late classes of RNA from vaccinia virus-infected cells. Virology. 1979 Jul 30;96(2):368–380. [PubMed]
  • Desselberger U, Racaniello VR, Zazra JJ, Palese P. The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene. 1980 Feb;8(3):315–328. [PubMed]
  • Enami M, Luytjes W, Krystal M, Palese P. Introduction of site-specific mutations into the genome of influenza virus. Proc Natl Acad Sci U S A. 1990 May;87(10):3802–3805. [PMC free article] [PubMed]
  • Gorman CM, Moffat LF, Howard BH. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. [PMC free article] [PubMed]
  • Gotoh H, Shioda T, Sakai Y, Mizumoto K, Shibuta H. Rescue of Sendai virus from viral ribonucleoprotein-transfected cells by infection with recombinant vaccinia viruses carrying Sendai virus L and P/C genes. Virology. 1989 Aug;171(2):434–443. [PubMed]
  • Graham FL, Smiley J, Russell WC, Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977 Jul;36(1):59–74. [PubMed]
  • Greenspan D, Krystal M, Nakada S, Arnheiter H, Lyles DS, Palese P. Expression of influenza virus NS2 nonstructural protein in bacteria and localization of NS2 in infected eucaryotic cells. J Virol. 1985 Jun;54(3):833–843. [PMC free article] [PubMed]
  • Honda A, Uéda K, Nagata K, Ishihama A. RNA polymerase of influenza virus: role of NP in RNA chain elongation. J Biochem. 1988 Dec;104(6):1021–1026. [PubMed]
  • Karacostas V, Nagashima K, Gonda MA, Moss B. Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8964–8967. [PMC free article] [PubMed]
  • Kaverin NV, Varich NL, Surgay VV, Chernos VI. A quantitative estimation of poxvirus genome fraction transcribed as "early" and "late" mRNA. Virology. 1975 May;65(1):112–119. [PubMed]
  • Krystal M, Li R, Lyles D, Pavlakis G, Palese P. Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutants. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2709–2713. [PMC free article] [PubMed]
  • Lamb RA, Lai CJ. Expression of unspliced NS1 mRNA, spliced NS2 mRNA, and a spliced chimera mRNA from cloned influenza virus NS DNA in an SV40 vector. Virology. 1984 May;135(1):139–147. [PubMed]
  • Li RA, Palese P, Krystal M. Complementation and analysis of an NP mutant of influenza virus. Virus Res. 1989 Feb;12(2):97–111. [PubMed]
  • Luytjes W, Krystal M, Enami M, Parvin JD, Palese P. Amplification, expression, and packaging of foreign gene by influenza virus. Cell. 1989 Dec 22;59(6):1107–1113. [PubMed]
  • Moss B. Use of vaccinia virus vectors for development of AIDS vaccines. AIDS. 1988;2 (Suppl 1):S103–S105. [PubMed]
  • Oda KI, Joklik WK. Hybridization and sedimentation studies on "early" and "late" vaccinia messenger RNA. J Mol Biol. 1967 Aug 14;27(3):395–419. [PubMed]
  • Parvin JD, Palese P, Honda A, Ishihama A, Krystal M. Promoter analysis of influenza virus RNA polymerase. J Virol. 1989 Dec;63(12):5142–5152. [PMC free article] [PubMed]
  • Pattnaik AK, Wertz GW. Replication and amplification of defective interfering particle RNAs of vesicular stomatitis virus in cells expressing viral proteins from vectors containing cloned cDNAs. J Virol. 1990 Jun;64(6):2948–2957. [PMC free article] [PubMed]
  • Piccini A, Paoletti E. Vaccinia: virus, vector, vaccine. Adv Virus Res. 1988;34:43–64. [PubMed]
  • Piccini A, Perkus ME, Paoletti E. Vaccinia virus as an expression vector. Methods Enzymol. 1987;153:545–563. [PubMed]
  • del Río L, Martínez C, Domingo E, Ortín J. In vitro synthesis of full-length influenza virus complementary RNA. EMBO J. 1985 Jan;4(1):243–247. [PMC free article] [PubMed]
  • Shapiro GI, Gurney T, Jr, Krug RM. Influenza virus gene expression: control mechanisms at early and late times of infection and nuclear-cytoplasmic transport of virus-specific RNAs. J Virol. 1987 Mar;61(3):764–773. [PMC free article] [PubMed]
  • Shapiro GI, Krug RM. Influenza virus RNA replication in vitro: synthesis of viral template RNAs and virion RNAs in the absence of an added primer. J Virol. 1988 Jul;62(7):2285–2290. [PMC free article] [PubMed]
  • Shimizu K, Mullinix MG, Chanock RM, Murphy BR. Temperature-sensitive mutants of influenza A/Udorn/72 (H3N2) virus. III. Genetic analysis of temperature-dependent host range mutants. Virology. 1983 Jan 15;124(1):35–44. [PubMed]
  • Smith DB, Inglis SC. Regulated production of an influenza virus spliced mRNA mediated by virus-specific products. EMBO J. 1985 Sep;4(9):2313–2319. [PMC free article] [PubMed]
  • Smith GL, Levin JZ, Palese P, Moss B. Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses. Virology. 1987 Oct;160(2):336–345. [PubMed]
  • Ulmanen I, Broni BA, Krug RM. Role of two of the influenza virus core P proteins in recognizing cap 1 structures (m7GpppNm) on RNAs and in initiating viral RNA transcription. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7355–7359. [PMC free article] [PubMed]
  • Wolstenholme AJ, Barrett T, Nichol ST, Mahy BW. Influenza virus-specific RNA and protein syntheses in cells infected with temperature-sensitive mutants defective in the genome segment encoding nonstructural proteins. J Virol. 1980 Jul;35(1):1–7. [PMC free article] [PubMed]
  • Young JF, Desselberger U, Palese P, Ferguson B, Shatzman AR, Rosenberg M. Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6105–6109. [PMC free article] [PubMed]

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