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Journal List > J Bacteriol > v.108(1); Oct 1971

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J Bacteriol. 1971 October; 108(1): 69–73.
PMCID: PMC247033
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Nystatin-Resistant Mutants of Yeast: Alterations in Sterol Content
Robin A. Woods
1Department of Genetics, The University of Sheffield, Sheffield S10 2TN, England
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Abstract
Mutants of the genes nys1 and nys3 differ from sensitive strains (nys+) in their sterol content. Ultraviolet absorption spectra of the nonsaponifiable material extracted from cells of nys+ demonstrated the presence of ergosterol and 24(28)-dehydroergosterol. In nys1 mutants, the spectrum suggests the presence of a new sterol. The absorption spectrum of extracts from nys3 mutants indicates absence of both ergosterol and 24(28)-dehydroergosterol and presence of another new sterol. Conversion of nys+ and nys3 to petite results in loss of 24(28)-dehydroergosterol in the former and the new sterol in the latter, whereas the new sterol in nys1 is only reduced. The sterols in ethanol-grown cells of all genotypes are essentially the same as is found for growth on glucose. With the exception of nys3 grown on ethanol, the mutants do not appear to be at a disadvantage compared to wild type.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • ADELBERG EA, ROSLANSKY PF, MYERS JW, COUGHLIN CA. Attempted induction of microbial mutants requiring steroidal growth factors. J Bacteriol. 1955 Jun;69(6):733. [PubMed]
  • Ahmed KA, Woods RA. A genetic analysis of resistance to nystatin in Saccharomyces cerevisiae. Genet Res. 1967 Apr;9(2):179–193. [PubMed]
  • GOTTLIEB D, CARTER HE, SLONEKER JH, AMMANN A. Protection of fungi against polyene antibiotics by sterols. Science. 1958 Aug 15;128(3320):361–361. [PubMed]
  • Katsuki H, Bloch K. Studies on the biosynthesis of ergosterol in yeast. Formation of methylated intermediates. J Biol Chem. 1967 Jan 25;242(2):222–227. [PubMed]
  • Kinsky SC. Antibiotic interaction with model membranes. Annu Rev Pharmacol. 1970;10:119–142. [PubMed]
  • LAMPEN JO, ARNOW PM, BOROWSKA Z, LASKIN AI. Location and role of sterol at nystatin-binding sites. J Bacteriol. 1962 Dec;84:1152–1160. [PubMed]
  • LAMPEN JO, ARNOW PM, SAFFERMAN RS. Mechanism of protection by sterols against polyene antibiotics. J Bacteriol. 1960 Aug;80:200–206. [PubMed]
  • Longley RP, Rose AH, Knights BA. Composition of the protoplast membrane from Saccharomyces cerevisiae. Biochem J. 1968 Jul;108(3):401–412. [PubMed]
  • Resnick MA, Mortimer RK. Unsaturated fatty acid mutants of Saccharomyces cerevisiae. J Bacteriol. 1966 Sep;92(3):597–600. [PubMed]
  • Woods RA, Ahmed KA. Genetically controlled cross resistance to polyene antibiotics in Saccharomyces cerevisiae. Nature. 1968 Apr 27;218(5139):369–370. [PubMed]
  • Zygmunt Walter A, Tavormina Peter A. Steroid Interference with Antifungal Activity of Polyene Antibiotics. Appl Microbiol. 1966 Nov;14(6):865–869. [PubMed]
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