• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Oct 1973; 116(1): 378–383.
PMCID: PMC246433

Utilization of Octopine and Nopaline by Agrobacterium


Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utilized neither compound, while three strains were found which could utilize both. Of the remaining virulent strains, 27 utilized octopine and 14 utilized nopaline. Each of six strains of A. rhizogenes tested utilized only octopine but at a slower rate relative to growth than most A. tumefaciens. All eight of the A. radiobacter strains failed to utilize either compound, as did four of six nonvirulent strains of A. tumefaciens. The rhizobia did not utilize octopine or, with the possible exception of R. japonicum, nopaline. Virulence in the genus Agrobacterium is concluded to be highly correlated with the ability to utilize one or both of these compounds.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (885K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Lippincott JA, Lippincott BB. Lysopine and Octopine Promote Crown-Gall Tumor Growth in vivo. Science. 1970 Oct 9;170(3954):176–177. [PubMed]
  • Lippincott JA, Lippincott BB, Chang CC. Promotion of crown-gall tumor growth by lysopine, octopine, nopaline, and carnosine. Plant Physiol. 1972 Feb;49(2):131–137. [PMC free article] [PubMed]
  • MacGregor AN, Alexander M. Formation of tumor-like structures on legume roots by Rhizobium. J Bacteriol. 1971 Mar;105(3):728–732. [PMC free article] [PubMed]
  • Petit A, Tourneur J. Perte de virulence associée à la perte d'une activité enzymatique chez Agrobacterium tumefaciens. C R Acad Sci Hebd Seances Acad Sci D. 1972 Jul 3;275(1):137–139. [PubMed]
  • Rigaud J. Croissance, teneur en auxine et catabolisme auxinique chez Rhizobium. Arch Mikrobiol. 1969;66(1):29–33. [PubMed]
  • ROSENBERG H, ENNOR AH, MORRISON JF. The estimation of arginine. Biochem J. 1956 May;63(1):153–159. [PMC free article] [PubMed]
  • NGUYEN VAN THOAI, ROBIN Y. [Metabolism of guanidyl derivatives. IX. Biosynthesis of octopine: study of the mechanism of the reaction and some properties of octopine synthetase]. Biochim Biophys Acta. 1961 Sep 16;52:221–233. [PubMed]
  • VAN LANEN JM, RIKER AJ, BALDWIN IL. The effect of amino acids and related compounds upon the growth, virulence, and enzyme activity of crown gall bacteria. J Bacteriol. 1952 Jun;63(6):723–734. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...