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J Bacteriol. Nov 1974; 120(2): 631–637.
PMCID: PMC245821

Subcellular Localization of Isoleucine-Valine Biosynthetic Enzymes in Yeast1

Abstract

By using the method of stepwise homogenization of yeast spheroplast lysates employed previously with the leucine biosynthetic enzymes, it is shown that threonine deaminase, acetohydroxy acid synthase, Mg2+-dependent isomero-reductase, and dihydroxy acid dehydratase are particulate. Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.

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Selected References

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  • BAUERLE RH, FRUENDLICH M, STORMER FC, UMBARGER HE. CONTROL OF ISOLEUCINE, VALINE AND LEUCINE BIOSYNTHESIS. II. ENDPRODUCT INHIBITION BY VALINE OF ACETOHYDROXY ACID SYNTHETASE IN SALMONELLA TYPHIMURIUM. Biochim Biophys Acta. 1964 Oct 23;92:142–149. [PubMed]
  • Cassady WE, Leiter EH, Bergquist A, Wagner RP. Separation of mitochondrial membranes of Neurospora crassa. II. Submitochondrial localization of the isoleucine-valine biosynthetic pathway. J Cell Biol. 1972 Apr;53(1):66–72. [PMC free article] [PubMed]
  • Hatfield GW, Umbarger HE. Threonine deaminase from Bacillus subtilis. I. Purification of the enzyme. J Biol Chem. 1970 Apr 10;245(7):1736–1741. [PubMed]
  • Kellems RE, Allison VF, Butow RA. Cytoplasmic type 80 S ribosomes associated with yeast mitochondria. II. Evidence for the association of cytoplasmic ribosomes with the outer mitochondrial membrane in situ. J Biol Chem. 1974 May 25;249(10):3297–3303. [PubMed]
  • Kellems RE, Butow RA. Cytoplasmic type 80 S ribosomes associated with yeast mitochondria. 3. Changes in the amount of bound ribosomes in response to changes in metabolic state. J Biol Chem. 1974 May 25;249(10):3304–3310. [PubMed]
  • Kim IC, Beattie DS. Formation of the yeast mitochondrial membrane. 1. Effects of inhibitors of protein synthesis on the kinetics of enzyme appearance during glucose derepression. Eur J Biochem. 1973 Jul 16;36(2):509–518. [PubMed]
  • Kline EL, Brown CS, Coleman WG, Jr, Umbarger HE. Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12. Biochem Biophys Res Commun. 1974 Apr 23;57(4):1144–1151. [PubMed]
  • Kuwana H, Caroline DF, Harding RW, Wagner RP. An acetohydroxy acid synthetase from Neurospora crassa. Arch Biochem Biophys. 1968 Oct;128(1):184–193. [PubMed]
  • Magee PT, Robichon-Szulmajster H. The regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. 2. Identification and characterization of mutants lacking the acetohydroxyacid synthetase. Eur J Biochem. 1968 Feb;3(4):502–506. [PubMed]
  • Magee PT, Robichon-Szulmajster H. The regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. 3. Properties and regulation of the activity of acetohydroxyacid synthetase. Eur J Biochem. 1968 Feb;3(4):507–511. [PubMed]
  • Marco R, Pestaña A, Sebastian J, Sols A. Oxaloacetate metabolic crossroads in liver. Enzyme compartmentation and regulation of gluconeogenesis. Mol Cell Biochem. 1974 Mar 8;3(1):53–70. [PubMed]
  • NOLTMANN EA, GUBLER CJ, KUBY SA. Glucose 6-phosphate dehydrogenase (Zwischenferment). I. Isolation of the crystalline enzyme from yeast. J Biol Chem. 1961 May;236:1225–1230. [PubMed]
  • Olshan AR, Gross SR. Role of the leu-3 cistron in the regulation of the synthesis of isoleucine and valine biosynthetic enzymes of Neurospora. J Bacteriol. 1974 May;118(2):374–384. [PMC free article] [PubMed]
  • RADHAKRISHANAN AN, WAGNER RP, SNELL EE. Biosynthesis of valine and i43soleucine, 3. alpha-Keto-beta-hydroxy acid reductase and alpha-hydroxy-beta-Keto acid reductoisomerase. J Biol Chem. 1960 Aug;235:2322–2331. [PubMed]
  • Ryan ED, Tracy JW, Kohlhaw GB. Subcellular localization of the leucine biosynthetic enzymes in yeast. J Bacteriol. 1973 Oct;116(1):222–225. [PMC free article] [PubMed]
  • Serrano R, Gancedo JM, Gancedo C. Assay of yeast enzymes in situ. A potential tool in regulation studies. Eur J Biochem. 1973 May 2;34(3):479–482. [PubMed]
  • Takenaka S, Kuwana H. Control of acetohydroxy acid synthetase in Neurospora crassa. J Biochem. 1972 Nov;72(5):1139–1145. [PubMed]
  • UMBARGER HE, BROWN B, EYRING EJ. Isoleucine and valine metabolism in Escherichia coli. IX. Utilization of acetolactate and acetohydroxybutyrate. J Biol Chem. 1960 May;235:1425–1432. [PubMed]
  • Weitzman PD, Hewson JK. In situ regulation of yeast citrate synthase. Absence of ATP inhibition observed in vitro. FEBS Lett. 1973 Oct 15;36(2):227–231. [PubMed]

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