• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aacPermissionsJournals.ASM.orgJournalAAC ArticleJournal InfoAuthorsReviewers
Antimicrob Agents Chemother. Feb 1991; 35(2): 272–276.
PMCID: PMC244990

Chromosomally mediated beta-lactamase production and gentamicin resistance in Enterococcus faecalis.

Abstract

We have analyzed four distinct strains of multiply resistant, beta-lactamase-producing enterococci isolated during an outbreak of colonization with these strains on an infant-toddler surgical ward at The Children's Hospital in Boston, Mass. All four strains were resistant to erythromycin, penicillin, and tetracycline and to high levels of gentamicin and streptomycin. One strain was also resistant to chloramphenicol. Plasmid profiles revealed four different plasmid patterns, with the number of identified plasmids ranging from zero to three. The gene coding for beta-lactamase production could be transferred at low frequency (less than 10(-8)) to an enterococcal recipient from one strain in conjunction with all of the other resistance determinants. Probes derived from the staphylococcal beta-lactamase gene and gentamicin resistance gene failed to hybridize with any of the detectable plasmids, but both genes were present on restriction fragments of genomic DNA in all strains. Our results indicate that the beta-lactamase genes and gentamicin resistance genes in these strains are integrated into the bacterial chromosome. The cotransmissibility of the resistance determinants raises the possibility of their incorporation into a multiresistance transposable genetic element.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Asheshov EH. Chromosomal location of the genetic elements controlling penicillinase production in a strain of Staphylococcus aureus. Nature. 1966 May 21;210(5038):804–806. [PubMed]
  • Clewell DB. Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiol Rev. 1981 Sep;45(3):409–436. [PMC free article] [PubMed]
  • Courvalin PM, Carlier C, Chabbert YA. Plasmid-linked tetracycline and erythromycin resistance in group D "streptococcus". Ann Inst Pasteur (Paris) 1972 Dec;123(6):755–759. [PubMed]
  • Courvalin PM, Shaw WV, Jacob AE. Plasmid-mediated mechanisms of resistance to aminoglycoside-aminocyclitol antibiotics and to chloramphenicol in group D streptococci. Antimicrob Agents Chemother. 1978 May;13(5):716–725. [PMC free article] [PubMed]
  • Eliopoulos GM, Wennersten C, Zighelboim-Daum S, Reiszner E, Goldmann D, Moellering RC., Jr High-level resistance to gentamicin in clinical isolates of Streptococcus (Enterococcus) faecium. Antimicrob Agents Chemother. 1988 Oct;32(10):1528–1532. [PMC free article] [PubMed]
  • Ferretti JJ, Gilmore KS, Courvalin P. Nucleotide sequence analysis of the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase 2"-aminoglycoside phosphotransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. J Bacteriol. 1986 Aug;167(2):631–638. [PMC free article] [PubMed]
  • Franke AE, Clewell DB. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. J Bacteriol. 1981 Jan;145(1):494–502. [PMC free article] [PubMed]
  • Johnston LH, Dyke KG. Staphylococcal penicillinase plasmids: studies on the reversion of a temperature-sensitive replication mutant to temperature stability. J Gen Microbiol. 1974 Jun;82(2):309–317. [PubMed]
  • LeBlanc DJ, Inamine JM, Lee LN. Broad geographical distribution of homologous erythromycin, kanamycin, and streptomycin resistance determinants among group D streptococci of human and animal origin. Antimicrob Agents Chemother. 1986 Apr;29(4):549–555. [PMC free article] [PubMed]
  • Lyon BR, Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev. 1987 Mar;51(1):88–134. [PMC free article] [PubMed]
  • McLaughlin JR, Murray CL, Rabinowitz JC. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. J Biol Chem. 1981 Nov 10;256(21):11283–11291. [PubMed]
  • Moellering RC, Jr, Wennersten C, Weinberg AN. Synergy of penicillin and gentamicin against Enterococci. J Infect Dis. 1971 Dec;124 (Suppl):S207–S209. [PubMed]
  • Murray BE, Mederski-Samaroj B. Transferable beta-lactamase. A new mechanism for in vitro penicillin resistance in Streptococcus faecalis. J Clin Invest. 1983 Sep;72(3):1168–1171. [PMC free article] [PubMed]
  • Murray BE, Mederski-Samoraj B, Foster SK, Brunton JL, Harford P. In vitro studies of plasmid-mediated penicillinase from Streptococcus faecalis suggest a staphylococcal origin. J Clin Invest. 1986 Jan;77(1):289–293. [PMC free article] [PubMed]
  • Patterson JE, Masecar BL, Zervos MJ. Characterization and comparison of two penicillinase-producing strains of Streptococcus (Enterococcus) faecalis. Antimicrob Agents Chemother. 1988 Jan;32(1):122–124. [PMC free article] [PubMed]
  • Patterson JE, Wanger A, Zscheck KK, Zervos MJ, Murray BE. Molecular epidemiology of beta-lactamase-producing enterococci. Antimicrob Agents Chemother. 1990 Feb;34(2):302–305. [PMC free article] [PubMed]
  • Rowland SJ, Dyke KG. Characterization of the staphylococcal beta-lactamase transposon Tn552. EMBO J. 1989 Sep;8(9):2761–2773. [PMC free article] [PubMed]
  • Schwesinger MD, Novick RP. Prophage-dependent plasmid integration in Staphylococcus aureus. J Bacteriol. 1975 Aug;123(2):724–738. [PMC free article] [PubMed]
  • Weber DA, Goering RV. Tn4201, a beta-lactamase transposon in Staphylococcus aureus. Antimicrob Agents Chemother. 1988 Aug;32(8):1164–1169. [PMC free article] [PubMed]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...