• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Jun 1982; 43(6): 1434–1439.
PMCID: PMC244251

Solvent Production and Morphological Changes in Clostridium acetobutylicum

Abstract

The morphological and cytological changes which occurred in Clostridium acetobutylicum P262 during the production of acetone, butanol, and ethanol in an industrial fermentation medium were identified and correlated with the growth and physiological changes. The swollen, cigar-shaped clostridial forms were involved in the conversion of acids to neutral solvents, and there was a correlation between the number of clostridial forms and the production of solvents. Sporulation mutants which were unable to form clostridial stages (cls mutants) did not produce solvents. Oligosporogenous mutants which showed reduced clostridial stage formation produced intermediate levels of solvents. Sporulation mutants blocked after the clostridial stage, which were unable to form mature spores (spo mutants), produced normal levels of solvents.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allcock ER, Reid SJ, Jones DT, Woods DR. Autolytic Activity and an Autolysis-Deficient Mutant of Clostridium acetobutylicum. Appl Environ Microbiol. 1981 Dec;42(6):929–935. [PMC free article] [PubMed]
  • Barber JM, Robb FT, Webster JR, Woods DR. Bacteriocin production by Clostridium acetobutylicum in an industrial fermentation process. Appl Environ Microbiol. 1979 Mar;37(3):433–437. [PMC free article] [PubMed]
  • Davies R, Stephenson M. Studies on the acetone-butyl alcohol fermentation: Nutritional and other factors involved in the preparation of active suspensions of Cl. acetobutylicum (Weizmann). Biochem J. 1941 Dec;35(12):1320–1331. [PMC free article] [PubMed]
  • Doi RH, Brown LR, Rodgers G, Hsu Y. Bacillus subtilis mutant altered in spore morphology and in RNA polymerase activity. Proc Natl Acad Sci U S A. 1970 Jun;66(2):404–410. [PMC free article] [PubMed]
  • Hoeniger JF, Headley CL. Cytology of spore germination in Clostridium pectinovorum. J Bacteriol. 1968 Nov;96(5):1835–1847. [PMC free article] [PubMed]
  • Hsu EJ, Ordal ZJ. Comparative metabolism of vegetative and sporulating cultures of Clostridium thermosaccharolyticum. J Bacteriol. 1970 May;102(2):369–376. [PMC free article] [PubMed]
  • Kay D, Warren SC. Sporulation in Bacillus subtilis. Morphological changes. Biochem J. 1968 Oct;109(5):819–824. [PMC free article] [PubMed]
  • O'Brien RW, Morris JG. Oxygen and the growth and metabolism of Clostridium acetobutylicum. J Gen Microbiol. 1971 Nov;68(3):307–318. [PubMed]
  • Murray CD, Pun PP, Strauss N. Characterization of a rifampin-resistant, conditional asporogenous mutant of Bacillus subtilis. J Bacteriol. 1975 Jul;123(1):346–353. [PMC free article] [PubMed]
  • SMITH AG, ELLNER PD. Cytological observations on the sporulation process of Clostridium perfringens. J Bacteriol. 1957 Jan;73(1):1–7. [PMC free article] [PubMed]
  • Walker PD. Symposium on bacterial spores: I. Cytology of spore formation and germination. J Appl Bacteriol. 1970 Mar;33(1):1–12. [PubMed]
  • Webster JR, Reid SJ, Jones DT, Woods DR. Purification and Characterization of an Autolysin from Clostridium acetobutylicum. Appl Environ Microbiol. 1981 Feb;41(2):371–374. [PMC free article] [PubMed]
  • Zeikus JG. Chemical and fuel production by anaerobic bacteria. Annu Rev Microbiol. 1980;34:423–464. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats: