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Appl Environ Microbiol. May 1979; 37(5): 959–964.
PMCID: PMC243332

Dichloran-rose bengal medium for enumeration and isolation of molds from foods.


Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. Dichloran (2,6 dichloro-4-nitroaniline) restricted growth of Rhizopus stolonifer while allowing satisfactory growth of the other test molds. Three Rhizopus and Mucor species were encountered that were not inhibited by dichloran; these were controlled by the addition of rose bengal. The optimal medium, designated DRBC, contained 2 micrograms of dichloran and 25 micrograms of rose bengal per ml. DRBC, in pure culture tests and with food samples, restricted the colony size of spreading molds and recovered a wider range of species in higher numbers than other enumeration media.

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Selected References

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  • Bell DK, Crawford JL. A botran-amended medium for isolating Aspergillus flavus from peanuts and soil. Phytopathology. 1967 Sep;57(9):939–941. [PubMed]
  • Jarvis B. Comparison of an improved rose bengal-chlortetracycline agar with other media for the selective isolation and enumeration of moulds and yeasts in foods. J Appl Bacteriol. 1973 Dec;36(4):723–727. [PubMed]
  • Mossel DA, Kleynen-Semmeling AM, Vincentie HM, Beerens H, Catsaras M. Oxytetracycline-glucose-yeast extract agar for selective enumeration of moulds and yeasts in foods and clinical material. J Appl Bacteriol. 1970 Sep;33(3):454–457. [PubMed]
  • Mossel DA, Vega CL, Put HM. Further studies on the suitability of various media containing antibacterial antibiotics for the enumeration of moulds in food and food environments. J Appl Bacteriol. 1975 Aug;39(1):15–22. [PubMed]
  • Ottow JC, Glathe H. Rose bengal-malt extract-agar, a simple medium for the simultaneous isolation and enumeration of fungi and actinomycetes from soil. Appl Microbiol. 1968 Jan;16(1):170–171. [PMC free article] [PubMed]

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