• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Mar 1979; 37(3): 369–372.
PMCID: PMC243223

Induction of mutation in Escherichia coli by freeze-drying.

Abstract

The effect of freeze-drying on phenotypic reversion of amino acid auxotrophy to prototrophy was studied in Escherichia coli. In a radioresistant strain, E. coli H/r 30 (uvr+ exr+), which can repair the deoxyribonucleic acid damaged due to freeze-drying, an increased mutation frequency from auxotrophy to prototrophy was observed with increased time of freeze-drying of the cells. On the other hand, in a radiosensitive strain, E. coli NG 30 (recA), which cannot repair the damaged deoxyribonucleic acid due to a lack of repair enzyme system, no significant reversion occurred, although the survival rate was very low. The rate of phenotypic reversion dut to freeze-drying in both E. coli RIMD 0509109 (uvr+ exr+) and RIMD 0509115 (uvr exr+) was almost the same, indicating that the phenomenon is independent of the uvr character. From these results it is concluded that mutation was induced in E. coli cells during the rehydration when the damaged deoxyribonucleic acid was repaired by exr character of the cells. Thus, we propose that a serious consideration should be paid to the freeze-drying technique to preserve bacterial cells.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (529K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ando Y, Fukada E. Effects of drying on deoxyribonucleic acids. J Radiat Res. 1974 Dec;15(4):212–218. [PubMed]
  • Ashwood-Smith MJ, Grant E. Mutation induction in bacteria by freeze-drying. Cryobiology. 1976 Apr;13(2):206–213. [PubMed]
  • DEMEREC M, CAHN E. Studies of mutability in nutritionally deficient strains of Escherichia coli. I. Genetic analysis of five auxotrophic strains. J Bacteriol. 1953 Jan;65(1):27–36. [PMC free article] [PubMed]
  • Hill RF. Ultraviolet-induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability. Photochem Photobiol. 1965 Jun;4(3):563–568. [PubMed]
  • Kato T, Kondo S. Two types of x-ray-sensitive mutants of Escherichia coli B: their phenotypic characters compared with UV-sensitive mutants. Mutat Res. 1967 May-Jun;4(3):253–263. [PubMed]
  • Lücke-Huhle C, Jung H. Energy requirements for damaging DNA molecules. I. Energy transfer from metastable states of excited gases. Int J Radiat Biol Relat Stud Phys Chem Med. 1973 Sep;24(3):221–228. [PubMed]
  • Ohnishi T, Tanaka Y, Yoh M, Takeda Y, Miwatani T. Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli. J Bacteriol. 1977 Jun;130(3):1393–1396. [PMC free article] [PubMed]
  • Sinskey TJ, Silverman GJ. Characterization of injury incurred by Escherichia coli upon freeze-drying. J Bacteriol. 1970 Feb;101(2):429–437. [PMC free article] [PubMed]
  • Tanaka Y, Ohnishi T, Takeda Y, Miwatani T. Lethal effect of freeze-drying on radiation-sensitive mutants of Escherichia coli. Biken J. 1975 Dec;18(4):267–269. [PubMed]
  • WEBB SJ. BOUND WATER, METABOLITES AND GENETIC CONTINUITY. Nature. 1964 Jul 25;203:374–377. [PubMed]
  • Webb SJ. Mutation of bacterial cells by controlled desiccation. Nature. 1967 Mar 18;213(5081):1137–1139. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...