• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Dec 1977; 34(6): 773–776.
PMCID: PMC242746

Production of vitamin B-12 in tempeh, a fermented soybean food.


Several varieties of soybeans contained generally less than 1 ng of vitamin B-12 per g. It was found that use of a lactic fermentation typical of tropical conditions during the initial soaking of the soybeans did not influence the vitamin B-12 content of the resulting tempeh. Pure tempeh molds obtained from different sources did not produce vitamin B-12. It was found that the major source of vitamin B-12 in commercial tempeh purchased in Toronto, Canada, was a bacterium that accompanies the mold during fermentation. Reinoculation of the pure bacterium onto dehulled, hydrated, and sterilized soybeans resulted in the production of 148 ng of vitamin B-12 per g. The presence of the mold, along with the bacterium, did not inhibit or enhance production of vitamin B-12. Nutritionally significant amounts of vitamin B-12 were also found in the Indonesian fermented food, ontjom.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (636K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Skeggs HR. Microbiological assay of vitamin B12. Methods Biochem Anal. 1966;14:53–62. [PubMed]
  • van Veen AG, Steinkraus KH. Nutritive value and wholesomeness of fermented foods. J Agric Food Chem. 1970 Jul-Aug;18(4):576–578. [PubMed]
  • Wang HL, Ruttle DI, Hesseltine CW. Antibacterial compound from a soybean product fermented by Rhizopus oligosporus. Proc Soc Exp Biol Med. 1969 Jun;131(2):579–583. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links