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Appl Environ Microbiol. Apr 1983; 45(4): 1389–1393.
PMCID: PMC242467

Sporulation of Clostridium acetobutylicum P262 in a Defined Medium


A defined minimal sporulation medium for Clostridium acetobutylicum P262, which produces high levels of solvents, is described. The overall sporulation sequence was similar to that of other endospore-forming bacteria. However, we observed a presporulation stage, during which swollen phase-bright cells which contained large amounts of granulose formed. During sporulation, the initiation of spore coat formation occurred before the onset of cortex formation. Other Clostridium strains tested showed marked variations in ability to grow and sporulate in various minimal media.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allcock ER, Reid SJ, Jones DT, Woods DR. Autolytic Activity and an Autolysis-Deficient Mutant of Clostridium acetobutylicum. Appl Environ Microbiol. 1981 Dec;42(6):929–935. [PMC free article] [PubMed]
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  • O'Brien RW, Morris JG. Oxygen and the growth and metabolism of Clostridium acetobutylicum. J Gen Microbiol. 1971 Nov;68(3):307–318. [PubMed]
  • SMITH AG, ELLNER PD. Cytological observations on the sporulation process of Clostridium perfringens. J Bacteriol. 1957 Jan;73(1):1–7. [PMC free article] [PubMed]
  • Webster JR, Reid SJ, Jones DT, Woods DR. Purification and Characterization of an Autolysin from Clostridium acetobutylicum. Appl Environ Microbiol. 1981 Feb;41(2):371–374. [PMC free article] [PubMed]
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