Logo of emborepLink to Publisher's site
EMBO Rep. 2008 Jul; 9(7): 683–689.
Published online 2008 Jun 6. doi:  10.1038/embor.2008.73
PMCID: PMC2424280
Scientific Report

Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo


The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show that Prdm1 functions by acting as a transcriptional repressor and that slow-twitch-specific muscle gene expression is activated by Prdm1-mediated repression of the transcriptional repressor Sox6. Genes encoding fast-specific isoforms of sarcomeric proteins are ectopically expressed in the adaxial cells of ubotp39 mutant embryos. By using chromatin immunoprecipitation, we show that these are direct targets of Prdm1. Thus, Prdm1 promotes slow-twitch fibre differentiation by acting as a global repressor of fast-fibre-specific genes, as well as by abrogating the repression of slow-fibre-specific genes.

Keywords: skeletal muscle, fibre type, Blimp1/Prdm1, Sox6, zebrafish, u-boot


During vertebrate development, cells become committed to the myogenic fate through the activation of myogenic regulatory factors in the paraxial mesoderm. Subsequently, the committed cells or myoblasts differentiate into muscle fibres with distinct contractile speeds, the so-called slow- and fast-twitch fibres. Terminal differentiation of these different fibre types requires the expression of specific isoforms of sarcomeric proteins, such as the myosin light and heavy chains (MyLC and MyHC, respectively) and troponins. In the zebrafish embryo, progenitors of the slow- and fast-twitch fibres can be identified on the basis of their morphology and positioning within the segmental plate before somitogenesis (Devoto et al, 1996). Paraxial mesodermal cells that lie in direct contact with the notochord—designated adaxial cells—differentiate into slow myoblasts that migrate out through the developing myotome to form a superficial layer of slow-twitch fibres. These slow fibres are mononuclear, span the entire length of each somite and express the homeodomain protein Prox1, as well as the slow myosin heavy chain 1 (smyhc1) and the slow-specific Troponin C (stnnC; Devoto et al, 1996; Roy et al, 2001; Xu et al, 2006). The fast-twitch fibres derive from more laterally located paraxial mesodermal cells that start to differentiate in the wake of the migrating slow myoblasts (Blagden et al, 1997). In contrast to the slow myoblasts, fast-twitch myoblasts undergo fusion to generate multinucleated fibres (Moore et al, 2007; Srinivas et al, 2007) and express fast MyLC and MyHC isoforms, as well as troponin T3a (tnnt3a) and troponin I2 (tnni2) (Xu et al, 2000; Hsiao et al, 2003).

The specification of adaxial cells to follow the slow-twitch fibre differentiation programme depends crucially on inductive signals from the notochord and floorplate mediated by members of the Hedgehog protein family (Currie & Ingham, 1996; Blagden et al, 1997; Du et al, 1997; Lewis et al, 1999; Barresi et al, 2000; Wolff et al, 2003). Reception of the Hedgehog signals by adaxial cells results in the activation of transcription of the u-boot (ubo) gene, the function of which is both necessary and sufficient to drive slow-twitch differentiation in myoblasts (Roy et al, 2001; Baxendale et al, 2004). In ubotp39 mutants, presumptive slow-twitch fibres lose sMyHC and Prox1 expression, and seem to differentiate into fast-twitch fibres (Roy et al, 2001). The ubo gene encodes the B-lymphocyte-induced maturation protein Blimp1 or Prdm1, a PR-domain-containing protein, which, in mammals, is involved in the terminal differentiation process of B lymphocytes, the response to viral infection and primordial germ cell specification (Keller & Maniatis, 1991; Turner et al, 1994; Ohinata et al, 2005; Kallies & Nutt, 2007). In these contexts, Prdm1 has been shown to mediate transcriptional repression, acting as a scaffold that recruits co-repressors and chromatin-modifying enzymes to specific target genes (Yu et al, 2000; Gyory et al, 2004). Here, we investigate the nature of Prdm1 function and the regulatory networks underlying fibre type specification, and identify several direct targets of Prdm1.

Results And Discussion

Adaxial cells transform from slow into fast in ubotp39 mutants

In wild-type embryos, adaxial cells are characterized by their expression of a slow isoform of the MyHC—detected by the S58 antibody—but are devoid of staining with F310—a fast MyLC isoform-specific antibody. Differentiation of adaxial cells into slow-twitch muscle starts several hours earlier than that of the fast-twitch fibres. Previous studies of ubo mutants have shown that loss of Prdm1 expression causes adaxial cells to transform from slow- to fast-twitch character (Roy et al, 2001). Consistent with this, we found that adaxial cells were labelled with F310 in ubotp39 homozygotes well before the normal onset of fast-muscle differentiation. To confirm that this represents the precocious differentiation of adaxial cells into fast-twitch fibres, we constructed a reporter gene, using sequences upstream from the previously uncharacterized fMyHC gene, which drives green fluorescent protein (GFP) expression strictly in fast-muscle cells (Fig 1A–C). When injected into ubotp39 mutant embryos, this fMyHCx:GFP transgene was ectopically expressed in adaxial cells (Fig 1D), indicative of their transformation from slow to fast character (Fig 1E,F). By using in situ hybridization (ISH), we found that tnnt3a and tnni2, encoding fast-specific isoforms of troponin, were also ectopically expressed in the adaxial cells of ubotp39 mutants, whereas expression of the gene encoding MyLC (mylz2) was significantly elevated above the levels found in wild-type adaxial cells (Fig 1G–I). Thus, Prdm1 acts to repress fast-specific genes, as well as to promote expression of slow-specific genes in adaxial cells.

Figure 1
Fast-muscle-fibre-specific genes are ectopically expressed in the slow domain of ubotp39 mutant embryos. In situ hybridization showing fast-muscle-specific expression of the fast myosin heavy chain gene (fMyHCx) at the 18-somite stage in (A) lateral and ...

Prdm1 acts as a repressor to promote slow twitch fibre type

To investigate whether Prdm1 acts as an activator or repressor of transcription during slow-twitch muscle development in zebrafish, constructs in which the Prdm1 DNA-binding domain is fused to either the Engrailed (Eng) repressor or the VP16 activator domain (Kessler, 1997) were tested for their ability to substitute for the wild-type protein. Sequences encoding the fusion constructs were cloned downstream from a heat-shock-inducible promoter that simultaneously drives expression of the fluorescent protein tdTomato. Transient expression of the Eng–Prdm1 protein induced by heat shock was sufficient to rescue the expression of Prox1 in ubotp39 mutant embryos (Fig 2C). By contrast, transient expression of the Vp16–Prdm1 fusion protein was unable to rescue Prox1 expression or suppress myoblast fusion (Fig 2D,E), but was sufficient to activate fast-myosin expression. Taken together, these data indicate that Prdm1 acts as a repressor rather than an activator to promote slow-muscle differentiation, and show that activation of prox1 and smyhc1 expression is an indirect effect of Prdm1 activity. As the PR domain is absent from the Eng–Prdm1 protein fusion, it also follows that this domain is not essential for Prdm1 function, at least in this context. Consistent with this, previous studies have shown the PR domain to be dispensable for Prdm1 function in the context of β-interferon repression (Gyory et al, 2004).

Figure 2
Slow-fibre differentiation requires the repressive function of Prdm1. (A) Prox1 expression in slow-muscle fibres of wild-type (wt) embryos at 24 h. (B) ubotp39 mutant embryos lack Prox1-expressing slow fibres at 24 h. (C) Eng–Prdm1 expression, ...

Prdm1 regulates Sox6—a repressor of slow fibre identity

In mice, the transcription factor Sox6 acts as a repressor of fetal slow-twitch fibre differentiation (Hagiwara et al, 2005, 2007). We analysed expression of the zebrafish sox6 gene and found that it was expressed in the fast-muscle progenitor domain of the somites but excluded from adaxial cells (Fig 3A). In ubotp39 mutant embryos, by contrast, sox6 is ectopically expressed in adaxial cells, indicating that Prdm1 represses sox6 expression in slow-muscle progenitors. Forcing ectopic expression of sox6 in the adaxial cells of wild-type embryos caused an inhibition of Prox1 expression (Fig 3B). Conversely, morpholino-mediated knockdown of sox6 in ubotp39 embryos partly rescued Prox1 expression and restored expression of smyhc1 to normal levels in adaxial cells (Fig 3C). Although neither Prox1 nor smyhc1 was expressed ectopically in fast fibres in response to sox6 knockdown, robust expression of stnnC was induced in the fast muscle of both wild-type and smoothened (smo) mutant (that lack all Hedgehog signalling activity) embryos injected with the sox6 morpholino (Fig 3E,F). This disparity might reflect a differential sensitivity of these slow-twitch-specific genes to sox6 activity, revealed by incomplete knockdown by the morpholino. Taken together, these data indicate that Sox6 acts as a repressor of slow-twitch-specific gene expression and suggest that Prdm1 activates such expression by repressing transcription of sox6.

Figure 3
Slow-specific gene expression is upregulated in sox6 morphant embryos. (A) sox6 is expressed in the fast-muscle precursors (red arrowheads) in wild-type (wt) embryos and ectopically expressed in the adaxial cells (green arrowheads) in ubotp39 embryos ...

Fast-twitch-specific genes are direct targets of Prdm1

The coordinated repression of multiple genes encoding fast-twitch isoforms of sarcomeric proteins could be accomplished by Prdm1-mediated repression of a fast-specific global transcriptional activator; alternatively, Prdm1 might itself act directly to repress transcription of these genes. To distinguish between these two scenarios, we used chromatin immunoprecipitation (ChIP) to test for binding of the protein to upstream regulatory regions of the putative targets. A polyclonal antibody was raised against 186 amino acids from the Prdm1 PR domain; as expected, this labelled adaxial cell nuclei during early- and mid-myogenesis (Fig 4A), and bound to Prdm1 specifically in immunoprecipitation assays (Fig 4B). DNA from chromatin immunoprecipitated with this antibody was amplified by using primer pairs specific for sequences proximal to the transcription initiation sites of the mylz2, fMyHCx, tnnt3a and tnni2 genes. These sequences were all found to be enriched in the Prdm1-precipitated chromatin; by contrast, none of the slow-muscle-specific genes smyhc1, stnnC or Prox1 was enriched (Fig 4C). These data indicate that Prdm1 selectively binds to putative regulatory regions of fast-fibre-specific genes in vivo, suggesting that Prdm1 acts as a direct repressor of their transcription.

Figure 4
Prdm1 acts as a direct repressor of fast-fibre-specific gene expression in adaxial cells. (A) prdm1:GFP expression is specific to adaxial cells in the somite area at the eight- to ten-somite stage. A Prdm1-specific polyclonal antibody detects Prdm1 protein ...

The mylz2 promoter has functional Prdm1 binding sites

Expression of a GFP reporter gene containing 2.3 kb of the mylz2 promoter sequence is specifically repressed in adaxial cells by Prdm1 activity (Fig 4D). Although the consensus binding site for Prdm1 has not been determined in zebrafish, we identified five putative Prdm1-binding sites in this fragment, containing the GAAAG core of the sequence (A/C)AG(T/C)GAAAG(T/C)(T/G) that has been defined as mediating Prdm1-dependent gene regulation in mammals (Kuo & Calame, 2004). The introduction of point mutations in each of these five potential Prdm1-binding sites in this construct led to ectopic adaxial GFP expression in wild-type embryos, similar to that seen with the wild-type construct in ubo morphants (Fig 4D). This finding is consistent with Prdm1 acting directly to repress the mylz2 gene in adaxial cells at the 12-somite stage.

Identification of Prdm1 target genes by ChIP on chip

To confirm and extend the findings of our candidate gene analysis, we used a recently constructed zebrafish promoter array, consisting of 60-mer probes for more than 11,000 genes within the zebrafish genome (Wardle et al, 2006), to probe the DNA isolated by ChIP of myoblast extracts (supplementary information online, accession code GSE10883 at GEO). By setting the gene array threshold for enrichment to the level of mylz2, which was at the level of significant P-value 0.0078 and P[Xbar] 0.0075, we identified 381 putative target genes (supplementary information online). Gene ontology analysis showed various genes enriched in the ChIP-on-chip sample: 11% were documented transcription factors, 24% were new genes or genes without known function, 15% were genes encoding proteins with enzyme activity, such as kinases and phosphatases, whereas others had gene ontology terms linking them to the immune and haematopoietic systems, cell-cycle regulation or apoptosis. Significantly, we found several genes encoding fast-fibre-specific isoforms of sarcomeric proteins, including those encoding fast MyHC and troponins described above, whereas no genes encoding slow-specific sarcomeric proteins or Prox1 were identified (supplementary information online). Surprisingly, sox6 was not among the transcription factor-encoding genes identified in this analysis. However, we note that representation of regulatory regions on the gene array is restricted to sequences 9 kb upstream from the 5′ end of the complementary DNAs used in its design (Wardle et al, 2006). We have identified additional sox6 sequences 30 kb upstream from the transcription start site used in the array (J.v.H., S.E. & P.W.I., unpublished data); whether sox6 is a direct target of Prdm1 remains to be determined.


Our data underline the pivotal role of Prdm1 in switching between alternative muscle fibre type programmes in the zebrafish embryo. We have shown that it accomplishes its function in two ways: first, by repressing the transcription of a repressor of slow-specific gene transcription, sox6, in a manner analogous to its repression of Pax5 in B cells (Lin et al, 2002) and, second, by acting directly as a global repressor of fast-specific differentiation genes. Although Prdm1 is expressed in the myotome of the mouse (Chang et al, 2002; Vincent et al, 2005), at present it is unclear whether it has an analogous role in fibre type specification in amniotes. Our finding that Sox6 suppresses slow-twitch fibre specification in zebrafish, however, establishes that at least some aspects of the regulatory network underlying fibre type specification (Hagiwara et al, 2005, 2007) have been conserved in evolution.


Fish strains, cloning of gene promoters and injection of embryos. Zebrafish mutants ubotp39 and smob641, and the transgenic line Tg(actal:GFP)zf13 have been described previously (van Eeden et al, 1996; Higashijima et al, 1997; Barresi et al, 2000; Chen et al, 2001; Roy et al, 2001; Baxendale et al, 2004). The Tg(prdm1:gfp)i111 and Tg(prdm1:gfp)i106 are described by Elworthy et al (2008). The mylz2:GFP promoter construct was generated by using 2,239 bp of the mylz2 upstream region (Ju et al, 2003; Moore et al, 2007) to generate a stable line Tg(mylz2:GFP)i135. The mylz2:GFP plasmid was also used as a template for in vitro mutagenesis of the five sites containing the Prdm1 GAAAG core sequence using the QuikChange® Multi Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) following the manufacturer's instructions to generate the Tg((5M)mylz2:GFP)i130 transgenic line. A 6.8 kb fragment upstream from the fMyHCx ATG was isolated by PCR from bacterial artificial chromosome zCR392328 by using a left primer containing an AscI restriction site (TGGCGCGCCTGCATGGTGTTTGACA) and a right primer containing NcoI (ACCCATGGTGGCGGCTTACCGT). The promoter DNA was in all cases subcloned into a GFP vector with flanking I-sceI sites. One-cell-stage embryos were microinjected with 4–8 nl of plasmid at a concentration of 40 ng/nl. Embryos were kept at 28.5°C and analysed for GFP at the 12-somite stage. Ubo morpholino-mediated knockdown of Prdm1 activity was carried out as described previously (Baxendale et al, 2004). The sox6 translation targeted morpholino (GTGGCTTGCTTGGAAGACATGATTC) was injected into one-cell-stage embryos at 0.9 mM. All fish were raised, staged and maintained as described previously (Kimmel et al, 1995; Westerfield, 2000).

Prox1 rescue assay. Eng–Prdm1 and VP16–Prdm1 fusion constructs (gifts from Dr Johaness Bischof; for details, see the supplementary information online) were used in attempts to rescue Prox1 expression. Both constructs and the complete sox6 cds (EU532205) were subcloned into a pSGH2 vector containing a bidirectional heat-shock promoter (Bajoghli et al, 2004) that drives expression of both the fusion protein and the fluorescent protein tdTomato. One-cell-stage embryos were microinjected with plasmid at a concentration of 40 ng/μl. At the three- to four-somite stage, the injected embryos were heat shocked by incubation at 39°C for 2 h. Injected embryos were fixed in 4% paraformaldehyde at 24 hpf (hours post fertilization) and analysed by using confocal microscopy.

Antibodies, immunohistochemistry and ChIP. A Prdm1 polyclonal antibody was raised against a fragment of the protein corresponding to amino acids 161–346 expressed as a His-tag fusion protein using the pET19b vector (Novagen, Darmstadt, Germany). Immunoprecipitation using the Prdm1 antibody was carried out according to Link et al (2006) using crude protein extracts from zebrafish embryos at the ten-somite stage. The precipitated proteins were analysed on SDS–polyacrylamide gel electrophoresis gel after Coomassie staining.

A Prox1 antibody was raised against recombinant zebrafish Prox1 purified from Escherichia coli (A.M. Taylor & P.W.I., unpublished data). Whole-mount immunohistochemical analysis using F310 fast MyLC (1:50, DSHB), S58 slow MyHC (1:10, DSHB), Prox1 (1:5,000) and Prdm1 (1:15,000) antibodies was performed on embryos fixed in 4% paraformaldehyde (for protocols, see the supplementary information online). For ChIP analysis, α-actin:GFP embryos were injected with dominant negative Protein Kinase A (dnPKA) at the one- to two-cell stage and were kept in embryonic medium until the 12- to 14-somite stage. The chorions were removed using pronase and cells were fixed for 15 min in 1.85% formaldehyde. For the ChIP-on-chip assay (supplementary information online), the embryos were dissociated using collagenase and GFP-positive cells were isolated. In addition to the ChIP on chip, three replicates of 300 embryos were used in the ChIP assay, which was performed as described previously (Wardle et al, 2006) using the Prdm1 antibody or rabbit preserum. Precipitated chromatin was analysed using Custom TaqManR Assays (Applied Biosystems, Foster City, CA, USA), specific to regions within the first 1 kb of upstream sequences from the first codon of mylz2 (NM_131188), fMyHCx (EU115994), tnnt3a (NM_131565), tnni2 (NM_205742), stnnC (AF281003), prox1 (NM_131405) and sMyHC1 (NM_001020507; for oligonucleotides and probes, see the supplementary information online). For whole-mount ISH, antisense digoxigenin (DIG) probes for mylz2, tnnt3a, tnni2, fMyHCx, sox6 and stnnC (subcloned from IMAGE_6899234, zgc:86932; Thisse et al, 2001; Xu et al, 2006) were generated and the ISH was performed as described previously (Thisse & Thisse, 1998). Fluorescent ISH used anti-dig POD (Roche, Basel, Switzerland) at 1:10,000 with tyramide signal amplification (TSA) Cyanine 3 (Perkin Elmer, Waltham, MA, USA).

Supplementary information is available at EMBO reports online (http://www.emboreports.org).

Supplementary Material

Supplementary Information

Supplement Data 1


We thank J. Bischof for the Eng–Prdm1 and Vp16–Prdm1 fusion constructs, and A.M. Taylor for generating the His–Prdm1 fusion protein and the Prox1 antibody. This work was funded by a UK MRC Programme Grant (G0100151) to P.W.I., a Wellcome Trust Programme grant (077592) to J.C.S. and the EU FP6 ‘Cells into Organs' Network of Excellence. J.v.H. is a Wenner-Gren Foundation fellow.


The authors declare that they have no conflict of interest.


  • Bajoghli B, Aghaallaei N, Heimbucher T, Czerny T (2004) An artificial promoter construct for heat-inducible misexpression during fish embryogenesis. Dev Biol 271: 416–430 [PubMed]
  • Barresi MJ, Stickney HL, Devoto SH (2000) The zebrafish slow-muscle-omitted gene product is required for Hedgehog signal transduction and the development of slow muscle identity. Development 127: 2189–2199 [PubMed]
  • Baxendale S, Davison C, Muxworthy C, Wolff C, Ingham PW, Roy S (2004) The B-cell maturation factor Blimp-1 specifies vertebrate slow-twitch muscle fiber identity in response to Hedgehog signaling. Nat Genet 36: 88–93 [PubMed]
  • Blagden CS, Currie PD, Ingham PW, Hughes SM (1997) Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog. Genes Dev 11: 2163–2175 [PMC free article] [PubMed]
  • Chang DH, Cattoretti G, Calame KL (2002) The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Mech Dev 117: 305–309 [PubMed]
  • Chen W, Burgess S, Hopkins N (2001) Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity. Development 128: 2385–2396 [PubMed]
  • Currie PD, Ingham PW (1996) Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish. Nature 382: 452–455 [PubMed]
  • Devoto SH, Melancon E, Eisen JS, Westerfield M (1996) Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation. Development 122: 3371–3380 [PubMed]
  • Du SJ, Devoto SH, Westerfield M, Moon RT (1997) Positive and negative regulation of muscle cell identity by members of the hedgehog and TGF-β gene families. J Cell Biol 139: 145–156 [PMC free article] [PubMed]
  • Elworthy S, Hargrave M, Knight R, Mebus K, Ingham PW (2008) Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity. Development 135: 2115–2126 [PubMed]
  • Gyory I, Wu J, Fejér G, Seto E, Wright KL (2004) PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing. Nat Immunol 5: 299–308 [PubMed]
  • Hagiwara N, Ma B, Ly A (2005) Slow and fast fiber isoform gene expression is systematically altered in skeletal muscle of the Sox6 mutant, p100H. Dev Dyn 234: 301–311 [PubMed]
  • Hagiwara N, Yeh M, Liu A (2007) Sox6 is required for normal fiber type differentiation of fetal skeletal muscle in mice. Dev Dyn 236: 2062–2076 [PubMed]
  • Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G (1997) High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin. Dev Biol 192: 289–299 [PubMed]
  • Hsiao CD, Tsai WY, Horng LS, Tsai HJ (2003) Molecular structure and developmental expression of three muscle-type troponin T genes in zebrafish. Dev Dyn 227: 266–279 [PubMed]
  • Ju B, Chong SW, He J, Wang X, Xu Y, Wan H, Tong Y, Yan T, Korzh V, Gong Z (2003) Recapitulation of fast skeletal muscle development in zebrafish by transgenic expression of GFP under the mylz2 promoter. Dev Dyn 227: 14–26 [PubMed]
  • Kallies A, Nutt SL (2007) Terminal differentiation of lymphocytes depends on Blimp-1. Curr Opin Immunol 19: 156–162 [PubMed]
  • Keller AD, Maniatis T (1991) Identification and characterization of a novel repressor of β-interferon gene expression. Genes Dev 5: 868–879 [PubMed]
  • Kessler DS (1997) Siamois is required for formation of Spemann's organizer. Proc Natl Acad Sci USA 94: 13017–13022 [PMC free article] [PubMed]
  • Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF (1995) Stages of embryonic development of the zebrafish. Dev Dyn 203: 253–310 [PubMed]
  • Kuo TC, Calame KL (2004) B lymphocyte-induced maturation protein (Blimp)-1, IFN regulatory factor (IRF)-1, and IRF-2 can bind to the same regulatory sites. J Immunol 173: 5556–5563 [PubMed]
  • Lewis KE, Currie PD, Roy S, Schauerte H, Haffter P, Ingham PW (1999) Control of muscle cell-type specification in the zebrafish embryo by Hedgehog signalling. Dev Biol 216: 469–480 [PubMed]
  • Lin KI, Angelin-Duclos C, Kuo TC, Calame K (2002) Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells. Mol Cell Biol 22: 4771–4780 [PMC free article] [PubMed]
  • Link V, Shevchenko A, Heisenberg CP (2006) Proteomics of early zebrafish embryos. BMC Dev Biol 6: 1. [PMC free article] [PubMed]
  • Moore CA, Parkin CA, Bidet Y, Ingham PW (2007) A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion. Development 134: 3145–3153 [PubMed]
  • Ohinata Y et al. (2005) Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436: 207–213 [PubMed]
  • Roy S, Wolff C, Ingham PW (2001) The u-boot mutation identifies a Hedgehog-regulated myogenic switch for fiber-type diversification in the zebrafish embryo. Genes Dev 15: 1563–1576 [PMC free article] [PubMed]
  • Srinivas BP, Woo J, Leong WY, Roy S (2007) A conserved molecular pathway mediates myoblast fusion in insects and vertebrates. Nat Genet 39: 781–786 [PubMed]
  • Thisse B et al. (2001) Expression of the zebrafish genome during embryogenesis. ZFIN Direct Data Submission (http://zfin.org)
  • Thisse C, Thisse B (1998) High resolution whole-mount in situ hybridization. Zebrafish Sci Monitor 5: 8–9
  • Turner CA Jr, Mack DH, Davis MM (1994) Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells. Cell 77: 297–306 [PubMed]
  • van Eeden FJ et al. (1996) Mutations affecting somite formation and patterning in the zebrafish, Danio rerio. Development 123: 153–164 [PubMed]
  • Vincent SD, Dunn NR, Sciammas R, Shapiro-Shalef M, Davis MM, Calame K, Bikoff EK, Robertson EJ (2005) The zinc finger transcriptional repressor Blimp1/Prdm1 is dispensable for early axis formation but is required for specification of primordial germ cells in the mouse. Development 132: 1315–1325 [PubMed]
  • Wardle FC, Odom DT, Bell GW, Yuan B, Danford TW, Wiellette EL, Herbolsheimer E, Sive HL, Young RA, Smith JC (2006) Zebrafish promoter microarrays identify actively transcribed embryonic genes. Genome Biol 7: R71. [PMC free article] [PubMed]
  • Westerfield M (2000) The Zebrafish Book. Eugene, OR, USA: University of Oregon Press
  • Wolff C, Roy S, Ingham PW (2003) Multiple muscle cell identities induced by distinct levels and timing of hedgehog activity in the zebrafish embryo. Curr Biol 13: 1169–1181 [PubMed]
  • Xu J et al. (2006) Genomewide expression profiling in the zebrafish embryo identifies target genes regulated by Hedgehog signaling during vertebrate development. Genetics 174: 735–752 [PMC free article] [PubMed]
  • Xu Y, He J, Wang X, Lim TM, Gong Z (2000) Asynchronous activation of 10 muscle-specific protein (MSP) genes during zebrafish somitogenesis. Dev Dyn 219: 201–215 [PubMed]
  • Yu J, Angelin-Duclos C, Greenwood J, Liao J, Calame K (2000) Transcriptional repression by blimp-1 (PRDI-BF1) involves recruitment of histone deacetylase. Mol Cell Biol 20: 2592–2603 [PMC free article] [PubMed]

Articles from EMBO Reports are provided here courtesy of The European Molecular Biology Organization
PubReader format: click here to try


Save items

Related citations in PubMed

See reviews...See all...


  • BioProject
    BioProject links
  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • Gene
    Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
  • Gene (nucleotide)
    Gene (nucleotide)
    Records in Gene identified from shared sequence and PMC links.
  • GEO DataSets
    GEO DataSets
    Gene expression and molecular abundance data reported in the current articles that are also included in the curated Gene Expression Omnibus (GEO) DataSets.
  • GEO Profiles
    GEO Profiles
    Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
  • HomoloGene
    HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
  • MedGen
    Related information in MedGen
  • Nucleotide
    Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
  • Pathways + GO
    Pathways + GO
    Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
  • Protein
    Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
  • Taxonomy
    Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...