• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Sep 1982; 44(3): 640–646.
PMCID: PMC242070

Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels.


Clinical and nonclinical isolates of the lactose-positive Vibrio vulnificus were compared with Vibrio strains isolated from lesions on eels (Anguilla japonica) cultured commercially in Japan. Strains were compared phenotypically and antigenically, for pathogenicity to mice and eels, and for genetic relatedness. The strains isolated from diseased eels differed phenotypically from the original species description of V. vulnificus in that they were negative for indole production, ornithine decarboxylase activity, growth at 42 degrees C, and acid production from mannitol and sorbitol. No relationship between the surface antigens of V. vulnificus strains from environmental and clinical sources and the strains from diseased eels was observed. Typical V. vulnificus strains and the eel isolates were pathogenic to mice; however, only those strains originally isolated from diseased eels were found to be pathogenic to eels. Results of DNA-DNA competition experiments revealed that there was greater than 90% relative reassociation between clinical and nonclinical V. vulnificus and strains from diseased eels. Based on the results of the DNA-DNA competition experiments, we conclude that the strains isolated from diseased eels were V. vulnificus; however, the differences in phenotypic characteristics and eel pathogenicity indicated that these strains represent a different biogroup. Therefore, we propose that strains phenotypically similar to the type strain of the species (ATCC 27562) be classified as V. vulnificus biogroup 1 and the strains phenotypically similar to those isolated from diseased eels be classified as V. vulnificus biogroup 2 represented by the reference strain ATCC 33148.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (872K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brenner DJ, Fanning GR, Johnson KE, Citarella RV, Falkow S. Polynucleotide sequence relationships among members of Enterobacteriaceae. J Bacteriol. 1969 May;98(2):637–650. [PMC free article] [PubMed]
  • Hollis DG, Weaver RE, Baker CN, Thornsberry C. Halophilic Vibrio species isolated from blood cultures. J Clin Microbiol. 1976 Apr;3(4):425–431. [PMC free article] [PubMed]
  • Kelly MT, Avery DM. Lactose-positive Vibrio in seawater: a cause of pneumonia and septicemia in a drowning victim. J Clin Microbiol. 1980 Mar;11(3):278–280. [PMC free article] [PubMed]
  • Kelly MT, McCormick WF. Acute bacterial myositis caused by Vibrio vulnificus. JAMA. 1981 Jul 3;246(1):72–73. [PubMed]
  • Kreger A, Lockwood D. Detection of extracellular toxin(s) produced by Vibrio vulnificus. Infect Immun. 1981 Aug;33(2):583–590. [PMC free article] [PubMed]
  • Mandel M, Igambi L, Bergendahl J, Dodson ML, Jr, Scheltgen E. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol. 1970 Feb;101(2):333–338. [PMC free article] [PubMed]
  • Reichelt JL, Baumann P, Baumann L. Study of genetic relationships among marine species of the genera Beneckea and Photobacterium by means of in vitro DNA/DNA hybridization. Arch Microbiol. 1976 Oct 11;110(1):101–120. [PubMed]
  • Seidler RJ, Allen DA, Colwell RR, Joseph SW, Daily OP. Biochemical characteristics and virulence of environmental group F bacteria isolated in the United States. Appl Environ Microbiol. 1980 Oct;40(4):715–720. [PMC free article] [PubMed]
  • Sizemore RK, Stevenson LH. Method for the isolation of proteolytic marine bacteria. Appl Microbiol. 1970 Dec;20(6):991–992. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...