• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. May 1991; 65(5): 2220–2224.
PMCID: PMC240569

Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus.


We have constructed hybrid retrovirus packaging cell lines that express the gibbon ape leukemia virus env and the Moloney murine leukemia virus gag-pol proteins. These cells were used to produce a retrovirus vector at over 10(6) CFU/ml, with a host range that included rat, hamster, bovine, cat, dog, monkey, and human cells. The gag-pol and env expression plasmids were separately transfected to reduce the potential for helper virus production, which was not observed. The NIH 3T3 mouse cells from which the packaging lines were made are not infectable by gibbon ape leukemia virus; thus, the generation and spread of possible recombinant viruses in the packaging cells is greatly reduced. These simian virus-based packaging cells extend the host range of currently available murine and avian packaging cells and should be useful for efficient gene transfer into higher mammals.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (938K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Armentano D, Yu SF, Kantoff PW, von Ruden T, Anderson WF, Gilboa E. Effect of internal viral sequences on the utility of retroviral vectors. J Virol. 1987 May;61(5):1647–1650. [PMC free article] [PubMed]
  • Bakay B, Nyhan WL, Croce CM, Koprowski H. Reversion in expression of hypoxanthine-guanine phosphoribosyl transferase following cell hybridization. J Cell Sci. 1975 May;17(3):567–578. [PubMed]
  • Bender MA, Palmer TD, Gelinas RE, Miller AD. Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region. J Virol. 1987 May;61(5):1639–1646. [PMC free article] [PubMed]
  • Chattopadhyay SK, Lander MR, Gupta S, Rands E, Lowy DR. Origin of mink cytopathic focus-forming (MCF) viruses:comparison with ecotropic and xenotropic murine leukemia virus genomes. Virology. 1981 Sep;113(2):465–483. [PubMed]
  • Delassus S, Sonigo P, Wain-Hobson S. Genetic organization of gibbon ape leukemia virus. Virology. 1989 Nov;173(1):205–213. [PubMed]
  • Huu Duc-Nguyen, Rosenblum EN, Zeigel RF. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J Bacteriol. 1966 Oct;92(4):1133–1140. [PMC free article] [PubMed]
  • Fischinger PJ, Nomura S, Bolognesi DP. A novel murine oncornavirus with dual eco- and xenotropic properties. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5150–5155. [PMC free article] [PubMed]
  • Gelmann EP, Trainor CD, Wong-Staal F, Reitz MS. Molecular cloning of circular unintegrated DNA of two types of the SEATO strain of gibbon ape leukemia virus. J Virol. 1982 Oct;44(1):269–275. [PMC free article] [PubMed]
  • Goff S, Traktman P, Baltimore D. Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase. J Virol. 1981 Apr;38(1):239–248. [PMC free article] [PubMed]
  • Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275–1281. [PMC free article] [PubMed]
  • Lieber MM, Sherr CJ, Todaro GJ, Benveniste RE, Callahan R, Coon HG. Isolation from the asian mouse Mus caroli of an endogenous type C virus related to infectious primate type C viruses. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2315–2319. [PMC free article] [PubMed]
  • Lusky M, Botchan M. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature. 1981 Sep 3;293(5827):79–81. [PubMed]
  • Miller AD. Retrovirus packaging cells. Hum Gene Ther. 1990 Spring;1(1):5–14. [PubMed]
  • Miller AD, Bender MA, Harris EA, Kaleko M, Gelinas RE. Design of retrovirus vectors for transfer and expression of the human beta-globin gene. J Virol. 1988 Nov;62(11):4337–4345. [PMC free article] [PubMed]
  • Miller AD, Buttimore C. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol Cell Biol. 1986 Aug;6(8):2895–2902. [PMC free article] [PubMed]
  • Miller AD, Law MF, Verma IM. Generation of helper-free amphotropic retroviruses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol Cell Biol. 1985 Mar;5(3):431–437. [PMC free article] [PubMed]
  • Miller AD, Trauber DR, Buttimore C. Factors involved in production of helper virus-free retrovirus vectors. Somat Cell Mol Genet. 1986 Mar;12(2):175–183. [PubMed]
  • Nelson-Rees WA, Owens RB, Arnstein P, Kniazeff AJ. Source, alterations, characteristics and use of a new dog cell line (Cf2Th). In Vitro. 1976 Oct;12(10):665–669. [PubMed]
  • O'Hara B, Johann SV, Klinger HP, Blair DG, Rubinson H, Dunn KJ, Sass P, Vitek SM, Robins T. Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus. Cell Growth Differ. 1990 Mar;1(3):119–127. [PubMed]
  • O'Neill RR, Buckler CE, Theodore TS, Martin MA, Repaske R. Envelope and long terminal repeat sequences of a cloned infectious NZB xenotropic murine leukemia virus. J Virol. 1985 Jan;53(1):100–106. [PMC free article] [PubMed]
  • Pursel VG, Pinkert CA, Miller KF, Bolt DJ, Campbell RG, Palmiter RD, Brinster RL, Hammer RE. Genetic engineering of livestock. Science. 1989 Jun 16;244(4910):1281–1288. [PubMed]
  • Quade K. Transformation of mammalian cells by avian myelocytomatosis virus and avian erythroblastosis virus. Virology. 1979 Oct 30;98(2):461–465. [PubMed]
  • Russell WC, Newman C, Williamson DH. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975 Feb 6;253(5491):461–462. [PubMed]
  • Simonsen CC, Levinson AD. Isolation and expression of an altered mouse dihydrofolate reductase cDNA. Proc Natl Acad Sci U S A. 1983 May;80(9):2495–2499. [PMC free article] [PubMed]
  • Sommerfelt MA, Weiss RA. Receptor interference groups of 20 retroviruses plating on human cells. Virology. 1990 May;176(1):58–69. [PubMed]
  • Wei CM, Gibson M, Spear PG, Scolnick EM. Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1. J Virol. 1981 Sep;39(3):935–944. [PMC free article] [PubMed]
  • Wilson C, Reitz MS, Okayama H, Eiden MV. Formation of infectious hybrid virions with gibbon ape leukemia virus and human T-cell leukemia virus retroviral envelope glycoproteins and the gag and pol proteins of Moloney murine leukemia virus. J Virol. 1989 May;63(5):2374–2378. [PMC free article] [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...