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Appl Environ Microbiol. Sep 1983; 46(3): 549–552.
PMCID: PMC239314

Simple and rapid method for isolating large plasmid DNA from lactic streptococci.


A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.

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Selected References

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  • Scherwitz KM, Baldwin KA, McKay LL. Plasmid linkage of a bacteriocin-like substance in Streptococcus lactis subsp. diacetylactis strain WM4: transferability to Streptococcus lactis. Appl Environ Microbiol. 1983 May;45(5):1506–1512. [PMC free article] [PubMed]
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