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Appl Environ Microbiol. Jun 1986; 51(6): 1235–1238.
PMCID: PMC239051

Selective medium for isolation of Fusarium species and dematiaceous hyphomycetes from cereals.


A selective medium containing 2 micrograms of dichloran per ml, 200 micrograms of chloramphenicol per ml, and 1.5% bacteriological peptone was developed for the isolation of Fusarium species and dematiaceous hyphomycetes from cereals. The medium, designated DCPA, was shown to select against species of Aspergillus, Penicillium, Cladosporium, and mucoraceous fungi. DCPA was evaluated for use as an enumeration medium and compared satisfactorily with dichloran-rose bengal-chloramphenicol agar when both media were tested with a range of cereal samples. Fusarium species and dematiaceous hyphomycetes produced well-formed colonies with good conidial production on DCPA, permitting rapid identification of such isolates on this medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Frisvad JC. A selective and indicative medium for groups of Penicillium viridicatum producing different mycotoxins in cereals. J Appl Bacteriol. 1983 Jun;54(3):409–416. [PubMed]
  • King AD, Jr, Hocking AD, Pitt JI. Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Appl Environ Microbiol. 1979 May;37(5):959–964. [PMC free article] [PubMed]
  • Pier AC. Mycotoxins and animal health. Adv Vet Sci Comp Med. 1981;25:185–243. [PubMed]
  • Pitt JI, Hocking AD, Glenn DR. An improved medium for the detection of Aspergillus flavus and A. parasiticus. J Appl Bacteriol. 1983 Feb;54(1):109–114. [PubMed]

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