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J Virol. Aug 1993; 67(8): 5056–5061.
PMCID: PMC237897

Human immunodeficiency virus type 1 Vpu protein is an oligomeric type I integral membrane protein.


The human immunodeficiency virus type 1 Vpu protein is a 16-kDa phosphoprotein which enhances the efficiency of virion production and induces rapid degradation of CD4, the cellular receptor for human immunodeficiency virus. The topology of membrane-inserted Vpu was investigated by using in vitro-synthesized Vpu cotranslationally inserted into canine microsomal membranes. Proteolytic digestion and immunoprecipitation studies revealed that Vpu was a type I integral membrane protein, with the hydrophilic domain projecting from the cytoplasmic membrane face. In addition, several high-molecular-weight proteins containing Vpu were identified by chemical cross-linking. Such complexes also formed when wild-type Vpu and a Tat-Vpu fusion protein were coexpressed. Subsequent analysis by one- and two-dimensional electrophoresis revealed that these high-molecular-weight complexes consisted of homo-oligomers of Vpu. These findings indicate that Vpu is a type I integral membrane protein capable of multimerization.

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Selected References

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  • Chou PY, Fasman GD. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. [PubMed]
  • Gaedigk-Nitschko K, Ding MX, Levy MA, Schlesinger MJ. Site-directed mutations in the Sindbis virus 6K protein reveal sites for fatty acylation and the underacylated protein affects virus release and virion structure. Virology. 1990 Mar;175(1):282–291. [PubMed]
  • Goldstein DJ, Kulke R, Dimaio D, Schlegel R. A glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase. J Virol. 1992 Jan;66(1):405–413. [PMC free article] [PubMed]
  • Haeuptle MT, Flint N, Gough NM, Dobberstein B. A tripartite structure of the signals that determine protein insertion into the endoplasmic reticulum membrane. J Cell Biol. 1989 Apr;108(4):1227–1236. [PMC free article] [PubMed]
  • Hiebert SW, Paterson RG, Lamb RA. Identification and predicted sequence of a previously unrecognized small hydrophobic protein, SH, of the paramyxovirus simian virus 5. J Virol. 1985 Sep;55(3):744–751. [PMC free article] [PubMed]
  • Hiebert SW, Richardson CD, Lamb RA. Cell surface expression and orientation in membranes of the 44-amino-acid SH protein of simian virus 5. J Virol. 1988 Jul;62(7):2347–2357. [PMC free article] [PubMed]
  • High S, Flint N, Dobberstein B. Requirements for the membrane insertion of signal-anchor type proteins. J Cell Biol. 1991 Apr;113(1):25–34. [PMC free article] [PubMed]
  • High S, Tanner MJ. Human erythrocyte membrane sialoglycoprotein beta. The cDNA sequence suggests the absence of a cleaved N-terminal signal sequence. Biochem J. 1987 Apr 1;243(1):277–280. [PMC free article] [PubMed]
  • Horwitz BH, Burkhardt AL, Schlegel R, DiMaio D. 44-amino-acid E5 transforming protein of bovine papillomavirus requires a hydrophobic core and specific carboxyl-terminal amino acids. Mol Cell Biol. 1988 Oct;8(10):4071–4078. [PMC free article] [PubMed]
  • Klimkait T, Strebel K, Hoggan MD, Martin MA, Orenstein JM. The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release. J Virol. 1990 Feb;64(2):621–629. [PMC free article] [PubMed]
  • Morozov SY, Miroshnichenko NA, Solovyev AG, Zelenina DA, Fedorkin ON, Lukasheva LI, Grachev SA, Chernov BK. In vitro membrane binding of the translation products of the carlavirus 7-kDa protein genes. Virology. 1991 Aug;183(2):782–785. [PubMed]
  • Pinto LH, Holsinger LJ, Lamb RA. Influenza virus M2 protein has ion channel activity. Cell. 1992 May 1;69(3):517–528. [PubMed]
  • Reiss P, Lange JM, de Ronde A, de Wolf F, Dekker J, Danner SA, Debouck C, Goudsmit J. Antibody response to viral proteins U (vpu) and R (vpr) in HIV-1-infected individuals. J Acquir Immune Defic Syndr. 1990;3(2):115–122. [PubMed]
  • Remaut E, Stanssens P, Fiers W. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene. 1981 Oct;15(1):81–93. [PubMed]
  • Rice CM, Strauss JH. Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2062–2066. [PMC free article] [PubMed]
  • Sato T, Sakaguchi M, Mihara K, Omura T. The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane. EMBO J. 1990 Aug;9(8):2391–2397. [PMC free article] [PubMed]
  • Strebel K, Beck E, Strohmaier K, Schaller H. Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins. J Virol. 1986 Mar;57(3):983–991. [PMC free article] [PubMed]
  • Strebel K, Daugherty D, Clouse K, Cohen D, Folks T, Martin MA. The HIV 'A' (sor) gene product is essential for virus infectivity. Nature. 1987 Aug 20;328(6132):728–730. [PubMed]
  • Strebel K, Klimkait T, Maldarelli F, Martin MA. Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein. J Virol. 1989 Sep;63(9):3784–3791. [PMC free article] [PubMed]
  • Strebel K, Klimkait T, Martin MA. A novel gene of HIV-1, vpu, and its 16-kilodalton product. Science. 1988 Sep 2;241(4870):1221–1223. [PubMed]
  • Sugrue RJ, Hay AJ. Structural characteristics of the M2 protein of influenza A viruses: evidence that it forms a tetrameric channel. Virology. 1991 Feb;180(2):617–624. [PubMed]
  • Tifft CJ, Proia RL, Camerini-Otero RD. The folding and cell surface expression of CD4 requires glycosylation. J Biol Chem. 1992 Feb 15;267(5):3268–3273. [PubMed]
  • Tung FY, Abraham S, Sethna M, Hung SL, Sethna P, Hogue BG, Brian DA. The 9-kDa hydrophobic protein encoded at the 3' end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated. Virology. 1992 Feb;186(2):676–683. [PubMed]
  • Walter P, Blobel G. Preparation of microsomal membranes for cotranslational protein translocation. Methods Enzymol. 1983;96:84–93. [PubMed]
  • Willey RL, Maldarelli F, Martin MA, Strebel K. Human immunodeficiency virus type 1 Vpu protein regulates the formation of intracellular gp160-CD4 complexes. J Virol. 1992 Jan;66(1):226–234. [PMC free article] [PubMed]
  • Willey RL, Maldarelli F, Martin MA, Strebel K. Human immunodeficiency virus type 1 Vpu protein induces rapid degradation of CD4. J Virol. 1992 Dec;66(12):7193–7200. [PMC free article] [PubMed]
  • Yao XJ, Göttlinger H, Haseltine WA, Cohen EA. Envelope glycoprotein and CD4 independence of vpu-facilitated human immunodeficiency virus type 1 capsid export. J Virol. 1992 Aug;66(8):5119–5126. [PMC free article] [PubMed]
  • Zebedee SL, Lamb RA. Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions. J Virol. 1988 Aug;62(8):2762–2772. [PMC free article] [PubMed]

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