• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Jun 1993; 67(6): 2972–2980.
PMCID: PMC237633

Sequence and structure alignment of paramyxovirus hemagglutinin-neuraminidase with influenza virus neuraminidase.

Abstract

A model is proposed for the three-dimensional structure of the paramyxovirus hemagglutinin-neuraminidase (HN) protein. The model is broadly similar to the structure of the influenza virus neuraminidase and is based on the identification of invariant amino acids among HN sequences which have counterparts in the enzyme-active center of influenza virus neuraminidase. The influenza virus enzyme-active site is constructed from strain-invariant functional and framework residues, but in this model of HN, it is primarily the functional residues, i.e., those that make direct contact with the substrate sialic acid, which have identical counterparts in neuraminidase. The framework residues of the active site are different in HN and in neuraminidase and appear to be less strictly conserved within HN sequences than within neuraminidase sequences.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Air GM, Laver WG, Luo M, Stray SJ, Legrone G, Webster RG. Antigenic, sequence, and crystal variation in influenza B neuraminidase. Virology. 1990 Aug;177(2):578–587. [PubMed]
  • Air GM, Ritchie LR, Laver WG, Colman PM. Gene and protein sequence of an influenza neuraminidase with hemagglutinin activity. Virology. 1985 Aug;145(1):117–122. [PubMed]
  • Baker AT, Varghese JN, Laver WG, Air GM, Colman PM. Three-dimensional structure of neuraminidase of subtype N9 from an avian influenza virus. Proteins. 1987;2(2):111–117. [PubMed]
  • Bando H, Kondo K, Kawano M, Komada H, Tsurudome M, Nishio M, Ito Y. Molecular cloning and sequence analysis of human parainfluenza type 4A virus HN gene: its irregularities on structure and activities. Virology. 1990 Mar;175(1):307–312. [PubMed]
  • Basak S, Tomana M, Compans RW. Sialic acid is incorporated into influenza hemagglutinin glycoproteins in the absence of viral neuraminidase. Virus Res. 1985 Feb;2(1):61–68. [PubMed]
  • Burmeister WP, Ruigrok RW, Cusack S. The 2.2 A resolution crystal structure of influenza B neuraminidase and its complex with sialic acid. EMBO J. 1992 Jan;11(1):49–56. [PMC free article] [PubMed]
  • Chong AK, Pegg MS, Taylor NR, von Itzstein M. Evidence for a sialosyl cation transition-state complex in the reaction of sialidase from influenza virus. Eur J Biochem. 1992 Jul 1;207(1):335–343. [PubMed]
  • Colman PM, Varghese JN, Laver WG. Structure of the catalytic and antigenic sites in influenza virus neuraminidase. Nature. 1983 May 5;303(5912):41–44. [PubMed]
  • Curran MD, Clarke DK, Rima BK. The nucleotide sequence of the gene encoding the attachment protein H of canine distemper virus. J Gen Virol. 1991 Feb;72(Pt 2):443–447. [PubMed]
  • Dale B, Brown R, Miller J, White RT, Air GM, Cordell B. Nucleotide and deduced amino acid sequence of the influenza neuraminidase genes of two equine serotypes. Virology. 1986 Dec;155(2):460–468. [PubMed]
  • Fields S, Winter G, Brownlee GG. Structure of the neuraminidase gene in human influenza virus A/PR/8/34. Nature. 1981 Mar 19;290(5803):213–217. [PubMed]
  • Galen JE, Ketley JM, Fasano A, Richardson SH, Wasserman SS, Kaper JB. Role of Vibrio cholerae neuraminidase in the function of cholera toxin. Infect Immun. 1992 Feb;60(2):406–415. [PMC free article] [PubMed]
  • Gorman WL, Gill DS, Scroggs RA, Portner A. The hemagglutinin-neuraminidase glycoproteins of human parainfluenza virus type 1 and Sendai virus have high structure-function similarity with limited antigenic cross-reactivity. Virology. 1990 Mar;175(1):211–221. [PubMed]
  • GOTTSCHALK A. Neuraminidase; its substrate and mode of action. Adv Enzymol Relat Subj Biochem. 1958;20:135–146. [PubMed]
  • Griffin JA, Basak S, Compans RW. Effects of hexose starvation and the role of sialic acid in influenza virus release. Virology. 1983 Mar;125(2):324–334. [PubMed]
  • Harley VR, Ward CW, Hudson PJ. Molecular cloning and analysis of the N5 neuraminidase subtype from an avian influenza virus. Virology. 1989 Mar;169(1):239–243. [PubMed]
  • Hiebert SW, Paterson RG, Lamb RA. Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor. J Virol. 1985 Apr;54(1):1–6. [PMC free article] [PubMed]
  • Higgins DG, Sharp PM. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene. 1988 Dec 15;73(1):237–244. [PubMed]
  • Hoyer LL, Hamilton AC, Steenbergen SM, Vimr ER. Cloning, sequencing and distribution of the Salmonella typhimurium LT2 sialidase gene, nanH, provides evidence for interspecies gene transfer. Mol Microbiol. 1992 Apr;6(7):873–884. [PubMed]
  • Iorio RM, Syddall RJ, Glickman RL, Riel AM, Sheehan JP, Bratt MA. Identification of amino acid residues important to the neuraminidase activity of the HN glycoprotein of Newcastle disease virus. Virology. 1989 Nov;173(1):196–204. [PubMed]
  • Jorgensen ED, Collins PL, Lomedico PT. Cloning and nucleotide sequence of Newcastle disease virus hemagglutinin-neuraminidase mRNA: identification of a putative sialic acid binding site. Virology. 1987 Jan;156(1):12–24. [PubMed]
  • Kahn S, Colbert TG, Wallace JC, Hoagland NA, Eisen H. The major 85-kDa surface antigen of the mammalian-stage forms of Trypanosoma cruzi is a family of sialidases. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4481–4485. [PMC free article] [PubMed]
  • Lentz MR, Air GM, Laver WG, Webster RG. Sequence of the neuraminidase gene of influenza virus A/Tokyo/3/67 and previously uncharacterized monoclonal variants. Virology. 1984 May;135(1):257–265. [PubMed]
  • Lentz MR, Webster RG, Air GM. Site-directed mutation of the active site of influenza neuraminidase and implications for the catalytic mechanism. Biochemistry. 1987 Aug 25;26(17):5351–5358. [PubMed]
  • Pereira ME, Mejia JS, Ortega-Barria E, Matzilevich D, Prioli RP. The Trypanosoma cruzi neuraminidase contains sequences similar to bacterial neuraminidases, YWTD repeats of the low density lipoprotein receptor, and type III modules of fibronectin. J Exp Med. 1991 Jul 1;174(1):179–191. [PMC free article] [PubMed]
  • Miura N, Nakatani Y, Ishiura M, Uchida T, Okada Y. Molecular cloning of a full-length cDNA encoding the hemagglutinin-neuraminidase glycoprotein of Sendai virus. FEBS Lett. 1985 Aug 19;188(1):112–116. [PubMed]
  • Ollis DL, Cheah E, Cygler M, Dijkstra B, Frolow F, Franken SM, Harel M, Remington SJ, Silman I, Schrag J, et al. The alpha/beta hydrolase fold. Protein Eng. 1992 Apr;5(3):197–211. [PubMed]
  • Palese P, Compans RW. Inhibition of influenza virus replication in tissue culture by 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA): mechanism of action. J Gen Virol. 1976 Oct;33(1):159–163. [PubMed]
  • Palese P, Tobita K, Ueda M, Compans RW. Characterization of temperature sensitive influenza virus mutants defective in neuraminidase. Virology. 1974 Oct;61(2):397–410. [PubMed]
  • Pereira ME, Mejia JS, Ortega-Barria E, Matzilevich D, Prioli RP. The Trypanosoma cruzi neuraminidase contains sequences similar to bacterial neuraminidases, YWTD repeats of the low density lipoprotein receptor, and type III modules of fibronectin. J Exp Med. 1991 Jul 1;174(1):179–191. [PMC free article] [PubMed]
  • Roggentin P, Rothe B, Kaper JB, Galen J, Lawrisuk L, Vimr ER, Schauer R. Conserved sequences in bacterial and viral sialidases. Glycoconj J. 1989;6(3):349–353. [PubMed]
  • Roggentin P, Rothe B, Lottspeich F, Schauer R. Cloning and sequencing of a Clostridium perfringens sialidase gene. FEBS Lett. 1988 Sep 26;238(1):31–34. [PubMed]
  • Rothe B, Roggentin P, Frank R, Blöcker H, Schauer R. Cloning, sequencing and expression of a sialidase gene from Clostridium sordellii G12. J Gen Microbiol. 1989 Nov;135(11):3087–3096. [PubMed]
  • Sakaguchi T, Toyoda T, Gotoh B, Inocencio NM, Kuma K, Miyata T, Nagai Y. Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene. Virology. 1989 Apr;169(2):260–272. [PubMed]
  • Scheid A, Caliguiri LA, Compans RW, Choppin PW. Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology. 1972 Dec;50(3):640–652. [PubMed]
  • Taylor G, Vimr E, Garman E, Laver G. Purification, crystallization and preliminary crystallographic study of neuraminidase from Vibrio cholerae and Salmonella typhimurium LT2. J Mol Biol. 1992 Aug 20;226(4):1287–1290. [PubMed]
  • Tulip WR, Varghese JN, Baker AT, van Donkelaar A, Laver WG, Webster RG, Colman PM. Refined atomic structures of N9 subtype influenza virus neuraminidase and escape mutants. J Mol Biol. 1991 Sep 20;221(2):487–497. [PubMed]
  • van Wyke Coelingh KL, Winter CC, Murphy BR. Nucleotide and deduced amino acid sequence of hemagglutinin-neuraminidase genes of human type 3 parainfluenza viruses isolated from 1957 to 1983. Virology. 1988 Jan;162(1):137–143. [PubMed]
  • Varghese JN, Colman PM. Three-dimensional structure of the neuraminidase of influenza virus A/Tokyo/3/67 at 2.2 A resolution. J Mol Biol. 1991 Sep 20;221(2):473–486. [PubMed]
  • Varghese JN, Laver WG, Colman PM. Structure of the influenza virus glycoprotein antigen neuraminidase at 2.9 A resolution. Nature. 1983 May 5;303(5912):35–40. [PubMed]
  • Varghese JN, McKimm-Breschkin JL, Caldwell JB, Kortt AA, Colman PM. The structure of the complex between influenza virus neuraminidase and sialic acid, the viral receptor. Proteins. 1992 Nov;14(3):327–332. [PubMed]
  • Varghese JN, Webster RG, Laver WG, Colman PM. Structure of an escape mutant of glycoprotein N2 neuraminidase of influenza virus A/Tokyo/3/67 at 3 A. J Mol Biol. 1988 Mar 5;200(1):201–203. [PubMed]
  • Warner TG, Harris R, McDowell R, Vimr ER. Photolabelling of Salmonella typhimurium LT2 sialidase. Identification of a peptide with a predicted structural similarity to the active sites of influenza-virus sialidases. Biochem J. 1992 Aug 1;285(Pt 3):957–964. [PMC free article] [PubMed]
  • Waxham MN, Aronowski J. Identification of amino acids involved in the sialidase activity of the mumps virus hemagglutinin-neuraminadase protein. Virology. 1988 Nov;167(1):226–232. [PubMed]
  • Waxham MN, Aronowski J, Server AC, Wolinsky JS, Smith JA, Goodman HM. Sequence determination of the mumps virus HN gene. Virology. 1988 Jun;164(2):318–325. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...