Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Oct 1994; 68(10): 6794–6798.
PMCID: PMC237106

Recombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into viruslike particles and induces protection.


VP60, the unique component of rabbit hemorrhagic disease virus capsid, was expressed in the baculovirus system. The recombinant VP60, released in the supernatant of infected insect cells, assembled without the need of any other viral component to form viruslike particles (VLPs), structurally and immunologically indistinguishable from the rabbit hemorrhagic disease virion. Intramuscular vaccination of rabbits with the VLPs conferred complete protection in 15 days; this protection was found to be effective from the fifth day after VLP injection and was accompanied by a strong humoral response.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aponte C, Mattion NM, Estes MK, Charpilienne A, Cohen J. Expression of two bovine rotavirus non-structural proteins (NSP2, NSP3) in the baculovirus system and production of monoclonal antibodies directed against the expressed proteins. Arch Virol. 1993;133(1-2):85–95. [PubMed]
  • Argüello Villares JL. Viral haemorrhagic disease of rabbits: vaccination and immune response. Rev Sci Tech. 1991 Jun;10(2):459–480. [PubMed]
  • Beames B, Braunagel S, Summers MD, Lanford RE. Polyhedrin initiator codon altered to AUU yields unexpected fusion protein from a baculovirus vector. Biotechniques. 1991 Sep;11(3):378–383. [PubMed]
  • Boga JA, Marín MS, Casais R, Prieto M, Parra F. In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV). Virus Res. 1992 Oct;26(1):33–40. [PubMed]
  • Carter MJ, Routledge EG, Toms GL. Monoclonal antibodies to feline calicivirus. J Gen Virol. 1989 Aug;70(Pt 8):2197–2200. [PubMed]
  • Greenberg HB, Valdesuso JR, Kalica AR, Wyatt RG, McAuliffe VJ, Kapikian AZ, Chanock RM. Proteins of Norwalk virus. J Virol. 1981 Mar;37(3):994–999. [PMC free article] [PubMed]
  • Jiang X, Wang M, Graham DY, Estes MK. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 1992 Nov;66(11):6527–6532. [PMC free article] [PubMed]
  • Jiang X, Wang M, Wang K, Estes MK. Sequence and genomic organization of Norwalk virus. Virology. 1993 Jul;195(1):51–61. [PubMed]
  • Kitts PA, Ayres MD, Possee RD. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res. 1990 Oct 11;18(19):5667–5672. [PMC free article] [PubMed]
  • Labbé M, Charpilienne A, Crawford SE, Estes MK, Cohen J. Expression of rotavirus VP2 produces empty corelike particles. J Virol. 1991 Jun;65(6):2946–2952. [PMC free article] [PubMed]
  • López de Turiso JA, Cortés E, Martínez C, Ruiz de Ybáez R, Simarro I, Vela C, Casal I. Recombinant vaccine for canine parvovirus in dogs. J Virol. 1992 May;66(5):2748–2753. [PMC free article] [PubMed]
  • Luckow VA, Summers MD. High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors. Virology. 1989 May;170(1):31–39. [PubMed]
  • McCullough KC, Butcher R. Monoclonal antibodies against foot-and-mouth disease virus 146S and 12S particles. Arch Virol. 1982;74(1):1–9. [PubMed]
  • Meyers G, Wirblich C, Thiel HJ. Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome. Virology. 1991 Oct;184(2):664–676. [PubMed]
  • Meyers G, Wirblich C, Thiel HJ. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology. 1991 Oct;184(2):677–686. [PubMed]
  • Neill JD. Nucleotide sequence of the capsid protein gene of two serotypes of San Miguel sea lion virus: identification of conserved and non-conserved amino acid sequences among calicivirus capsid proteins. Virus Res. 1992 Jul;24(2):211–222. [PubMed]
  • Ohlinger VF, Haas B, Meyers G, Weiland F, Thiel HJ. Identification and characterization of the virus causing rabbit hemorrhagic disease. J Virol. 1990 Jul;64(7):3331–3336. [PMC free article] [PubMed]
  • Parra F, Boga JA, Marin MS, Casais R. The amino terminal sequence of VP60 from rabbit hemorrhagic disease virus supports its putative subgenomic origin. Virus Res. 1993 Mar;27(3):219–228. [PubMed]
  • Parra F, Prieto M. Purification and characterization of a calicivirus as the causative agent of a lethal hemorrhagic disease in rabbits. J Virol. 1990 Aug;64(8):4013–4015. [PMC free article] [PubMed]
  • Rodák L, Granátová M, Valícek L, Smíd B, Veselý T, Nevoránková Z. Monoclonal antibodies to rabbit haemorrhagic disease virus and their use in the diagnosis of infection. J Gen Virol. 1990 Nov;71(Pt 11):2593–2598. [PubMed]
  • Schaffer FL, Soergel ME. Single major polypeptide of a calicivirus: characterization by polyacrylamide gel electrophoresis and stabilization of virions by cross-linking with dimethyl suberimidate. J Virol. 1976 Sep;19(3):925–931. [PMC free article] [PubMed]
  • Vakharia VN, Snyder DB, He J, Edwards GH, Savage PK, Mengel-Whereat SA. Infectious bursal disease virus structural proteins expressed in a baculovirus recombinant confer protection in chickens. J Gen Virol. 1993 Jun;74(Pt 6):1201–1206. [PubMed]
  • Valícek L, Smíd B, Rodák L, Kudrna J. Electron and immunoelectron microscopy of rabbit haemorrhagic disease virus (RHDV). Arch Virol. 1990;112(3-4):271–275. [PubMed]
  • Vautherot JF, Madelaine MF, Boireau P, Laporte J. Bovine coronavirus peplomer glycoproteins: detailed antigenic analyses of S1, S2 and HE. J Gen Virol. 1992 Jul;73(Pt 7):1725–1737. [PubMed]
  • Vlak JM, Keus RJ. Baculovirus expression vector system for production of viral vaccines. Adv Biotechnol Processes. 1990;14:91–128. [PubMed]
  • Xu ZJ, Chen WX. Viral haemorrhagic disease in rabbits: a review. Vet Res Commun. 1989;13(3):205–212. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...