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Bull World Health Organ. 1975; 52(2): 223–231.
PMCID: PMC2366365

A single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen

Proposals for an assay method for the haemagglutinin content of influenza vaccines


Single-radial-diffusion techniques are proposed as possible alternatives to tests based on agglutination of erythrocytes for the assay of the haemagglutinin content of influenza vaccines. Two test procedures (microtest and macrotest) and the use of reference reagents to assay vaccines using these tests are described. The two tests are of similar reproducibility and accuracy but the macrotest is technically simpler to perform and results of assays may be obtained more rapidly.

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Selected References

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  • Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. [PubMed]
  • Brand CM, Skehel JJ. Crystalline antigen from the influenza virus envelope. Nat New Biol. 1972 Aug 2;238(83):145–147. [PubMed]
  • Aymard-Henry M, Coleman MT, Dowdle WR, Laver WG, Schild GC, Webster RG. Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ. 1973;48(2):199–202. [PMC free article] [PubMed]
  • DULBECCO R, VOGT M. One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells. J Exp Med. 1954 Feb;99(2):183–199. [PMC free article] [PubMed]

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