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Br J Cancer. Feb 2001; 84(4): 535–538.
PMCID: PMC2363762

Malignant melanoma is genetically distinct from clear cell sarcoma of tendons and aponeurosis (malignant melanoma of soft parts)

Abstract

Clear cell sarcoma of tendons and aponeuroses (malignant melanoma of soft parts) and conventional malignant melanoma may demonstrate significant morphologic overlap at the light microscopic and ultrastructural level. Consequently, the clinically relevant distinction between primary clear cell sarcoma and metastatic melanoma in the absence of a known primary cutaneous, mucosal or ocular tumour may occasionally cause diagnostic problems. A balanced translocation, t(12;22)(q13;q13), which can be detected, amongst others, using the reverse transcriptase polymerase chain reaction (RT-PCR) or fluorescent in situ hybridization (FISH), has been identified in a high percentage (50–75%) of clear cell sarcomas and is presumed to be tumour specific. Whether this chromosomal rearrangement is present in malignant melanoma has, to date, not as yet been studied by molecular genetic or molecular cytogenetic techniques. Using RT-PCR and FISH, a series of metastases from 25 known cutaneous melanomas and 8 melanoma cell lines (5 uveal and 3 cutaneous) were screened for the t(12;22)(q13;q13) translocation. Primers for RT-PCR were chosen based upon published breakpoint sequences. The Cosmids G9 and CCS2.2, corresponding to the 5′ region of EWS and 3′ region of ATF-1 respectively, were used as probes. The translocation was not identified in any of the melanomas or melanoma cell lines analysed in this study; in contrast this translocation was identified in 3 out of 5 clear cell sarcomas using these techniques. This allows distinction between translocation positive cases of clear cell sarcoma and malignant melanoma at a molecular genetic level. Consequently, in diagnostically challenging cases, this represents a valuable tool for the clinicopathologic differentiation between these two entities, with an important impact on patient management and prognosis. © 2001 Cancer Research Campaign http://www.bjcancer.com

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Selected References

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  • Bridge JA, Borek DA, Neff JR, Huntrakoon M. Chromosomal abnormalities in clear cell sarcoma. Implications for histogenesis. Am J Clin Pathol. 1990 Jan;93(1):26–31. [PubMed]
  • Bridge JA, Sreekantaiah C, Neff JR, Sandberg AA. Cytogenetic findings in clear cell sarcoma of tendons and aponeuroses. Malignant melanoma of soft parts. Cancer Genet Cytogenet. 1991 Mar;52(1):101–106. [PubMed]
  • Castellano M, Parmiani G. Genes involved in melanoma: an overview of INK4a and other loci. Melanoma Res. 1999 Oct;9(5):421–432. [PubMed]
  • Chung EB, Enzinger FM. Malignant melanoma of soft parts. A reassessment of clear cell sarcoma. Am J Surg Pathol. 1983 Jul;7(5):405–413. [PubMed]
  • De Beuckeleer LH, De Schepper AM, Vandevenne JE, Bloem JL, Davies AM, Oudkerk M, Hauben E, Van Marck E, Somville J, Vanel D, et al. MR imaging of clear cell sarcoma (malignant melanoma of the soft parts): a multicenter correlative MRI-pathology study of 21 cases and literature review. Skeletal Radiol. 2000 Apr;29(4):187–195. [PubMed]
  • De Waard-Siebinga I, Blom DJ, Griffioen M, Schrier PI, Hoogendoorn E, Beverstock G, Danen EH, Jager MJ. Establishment and characterization of an uveal-melanoma cell line. Int J Cancer. 1995 Jul 17;62(2):155–161. [PubMed]
  • ENZINGER FM. CLEAR-CELL SARCOMA OF TENDONS AND APONEUROSES. AN ANALYSIS OF 21 CASES. Cancer. 1965 Sep;18:1163–1174. [PubMed]
  • Epstein AL, Martin AO, Kempson R. Use of a newly established human cell line (SU-CCS-1) to demonstrate the relationship of clear cell sarcoma to malignant melanoma. Cancer Res. 1984 Mar;44(3):1265–1274. [PubMed]
  • Fletcher JA. Translocation (12;22)(q13-14;q12) is a nonrandom aberration in soft-tissue clear-cell sarcoma. Genes Chromosomes Cancer. 1992 Sep;5(2):184–184. [PubMed]
  • Fletcher JA, Kozakewich HP, Hoffer FA, Lage JM, Weidner N, Tepper R, Pinkus GS, Morton CC, Corson JM. Diagnostic relevance of clonal cytogenetic aberrations in malignant soft-tissue tumors. N Engl J Med. 1991 Feb 14;324(7):436–442. [PubMed]
  • Graadt van Roggen JF, Mooi WJ, Hogendoorn PC. Clear cell sarcoma of tendons and aponeuroses (malignant melanoma of soft parts) and cutaneous melanoma: exploring the histogenetic relationship between these two clinicopathological entities. J Pathol. 1998 Sep;186(1):3–7. [PubMed]
  • Graadt van Roggen JF, Bovée JV, Morreau J, Hogendoorn PC. Diagnostic and prognostic implications of the unfolding molecular biology of bone and soft tissue tumours. J Clin Pathol. 1999 Jul;52(7):481–489. [PMC free article] [PubMed]
  • Kindblom LG, Lodding P, Angervall L. Clear-cell sarcoma of tendons and aponeuroses. An immunohistochemical and electron microscopic analysis indicating neural crest origin. Virchows Arch A Pathol Anat Histopathol. 1983;401(1):109–128. [PubMed]
  • Ladanyi M. The emerging molecular genetics of sarcoma translocations. Diagn Mol Pathol. 1995 Sep;4(3):162–173. [PubMed]
  • Limon J, Debiec-Rychter M, Nedoszytko B, Liberski PP, Babińska M, Szadowska A. Aberrations of chromosome 22 and polysomy of chromosome 8 as non-random changes in clear cell sarcoma. Cancer Genet Cytogenet. 1994 Feb;72(2):141–145. [PubMed]
  • Mooi WJ, Deenik W, Peterse JL, Hogendoorn PC. Keratin immunoreactivity in melanoma of soft parts (clear cell sarcoma). Histopathology. 1995 Jul;27(1):61–65. [PubMed]
  • Mrózek K, Karakousis CP, Perez-Mesa C, Bloomfield CD. Translocation t(12;22)(q13;q12.2-12.3) in a clear cell sarcoma of tendons and aponeuroses. Genes Chromosomes Cancer. 1993 Apr;6(4):249–252. [PubMed]
  • Nedoszytko B, Mrózek K, Roszkiewicz A, Kopacz A, Swierblewski M, Limon J. Clear cell sarcoma of tendons and aponeuroses with t(12;22) (q13;q12) diagnosed initially as malignant melanoma. Cancer Genet Cytogenet. 1996 Oct 1;91(1):37–39. [PubMed]
  • Pedersen MI, Bennett JW, Wang N. Nonrandom chromosome structural aberrations and oncogene loci in human malignant melanoma. Cancer Genet Cytogenet. 1986 Feb 1;20(1-2):11–27. [PubMed]
  • Peulvé P, Michot C, Vannier JP, Tron P, Hemet J. Clear cell sarcoma with t(12;22) (q13-14;q12). Genes Chromosomes Cancer. 1991 Sep;3(5):400–402. [PubMed]
  • Piepkorn M. Melanoma genetics: an update with focus on the CDKN2A(p16)/ARF tumor suppressors. J Am Acad Dermatol. 2000 May;42(5 Pt 1):705–726. [PubMed]
  • Reeves BR, Fletcher CD, Gusterson BA. Translocation t(12;22)(q13;q13) is a nonrandom rearrangement in clear cell sarcoma. Cancer Genet Cytogenet. 1992 Dec;64(2):101–103. [PubMed]
  • Rodriguez E, Sreekantaiah C, Reuter VE, Motzer RJ, Chaganti RS. t(12;22)(q13;q13) and trisomy 8 are nonrandom aberrations in clear-cell sarcoma. Cancer Genet Cytogenet. 1992 Dec;64(2):107–110. [PubMed]
  • Speleman F, Colpaert C, Goovaerts G, Leroy JG, Van Marck E. Malignant melanoma of soft parts. Further cytogenetic characterization. Cancer Genet Cytogenet. 1992 Jun;60(2):176–179. [PubMed]
  • Speleman F, Delattre O, Peter M, Hauben E, Van Roy N, Van Marck E. Malignant melanoma of the soft parts (clear-cell sarcoma): confirmation of EWS and ATF-1 gene fusion caused by a t(12;22) translocation. Mod Pathol. 1997 May;10(5):496–499. [PubMed]
  • Stenman G, Kindblom LG, Angervall L. Reciprocal translocation t(12;22)(q13;q13) in clear-cell sarcoma of tendons and aponeuroses. Genes Chromosomes Cancer. 1992 Mar;4(2):122–127. [PubMed]
  • Travis JA, Bridge JA. Significance of both numerical and structural chromosomal abnormalities in clear cell sarcoma. Cancer Genet Cytogenet. 1992 Dec;64(2):104–106. [PubMed]
  • Vaandrager JW, Schuuring E, Zwikstra E, de Boer CJ, Kleiverda KK, van Krieken JH, Kluin-Nelemans HC, van Ommen GJ, Raap AK, Kluin PM. Direct visualization of dispersed 11q13 chromosomal translocations in mantle cell lymphoma by multicolor DNA fiber fluorescence in situ hybridization. Blood. 1996 Aug 15;88(4):1177–1182. [PubMed]
  • Zucman J, Delattre O, Desmaze C, Epstein AL, Stenman G, Speleman F, Fletchers CD, Aurias A, Thomas G. EWS and ATF-1 gene fusion induced by t(12;22) translocation in malignant melanoma of soft parts. Nat Genet. 1993 Aug;4(4):341–345. [PubMed]

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