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Br J Cancer. 1999 March; 79(7/8): 1185–1189. doi: 10.1038/sj.bjc.6690190. | PMCID: PMC2362218 |
Overexpression of p53 protein in primary Ewing's sarcoma of bone: relationship to tumour stage, response and prognosis A Abudu,1 D C Mangham,1 G M Reynolds,1 P B Pynsent,1 R M Tillman,1 S R Carter,1 and R J Grimer1 1Royal Orthopaedic Hospital Oncology Service, Birmingham, B31 2AP, UK Received January 20, 1997; Revised July 14, 1998; Accepted August 3, 1998. Biopsy tissues of 52 patients with Ewing's sarcoma of bone treated between 1983 and 1993 were examined immunohistochemically to determine the significance of p53 protein in diagnosis and prognosis of Ewing's sarcoma. Mean age at diagnosis was 17 years (range 6–36) and minimum follow-up was 30 months. The tumours were located in the extremities and central bones in 35 and 17 patients respectively. Metastases were present in seven patients at diagnosis. Treatment consisted of chemotherapy, surgery and/or radiotherapy in all the patients. Overexpression of p53 protein was demonstrated in seven patients (14%). There was no relationship between expression of p53 and site of tumours. Patients who overexpressed p53 protein appeared to have more advanced diseases at diagnosis and poorer response to chemotherapy than those without p53 overexpression. The 5-year relapse-free survival and overall survival in patients without metastases at the time of diagnosis were 66% and 71%, respectively, in p53 protein-negative patients compared with 20% relapse-free and overall survival in those with p53 protein overexpression (P = 0.01). The poorer prognosis in p53 protein-positive patients was independent of site, local treatment or necrosis of the tumours (P < 0.05). Over-expression of p53 protein is an independent poor prognostic factor in Ewing's sarcoma of bone. © 1999 Cancer Research Campaign The Full Text of this article is available as a PDF (84K). These references are in PubMed. This may not be the complete list of references from this article. - Bartek J, Iggo R, Gannon J, Lane DP. Genetic and immunochemical analysis of mutant p53 in human breast cancer cell lines. Oncogene. 1990 Jun;5(6):893–899. [PubMed]
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