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J Bacteriol. Aug 1977; 131(2): 533–543.
PMCID: PMC235462

Fermentative metabolism of pyruvate by Rhodospirillum rubrum after anaerobic growth in darkness.


Rhodospirillum rubrum grew anaerobically in darkness and fermented sodium pyruvate by a pyruvate formate-lyase reaction. During 30 min of anaerobic dark or light incubation with sodium pyrivate, crude extracts from fermentatively grown cells produced about 6 micronmol of acetylphosphate and formate per mg of protein in reactions performed at pH 8.3. Cell extracts also catalyzed the exchange of sodium [14C]formate into sodium pyruvate at an apparent pH optimum of 7.3 to 7.5, but only about 2.5 micronmol of acetylphosphate was produced at this lower pH value. R. rubrum may also form pyruvate:ferredoxin oxidoreductase activity, as evidenced by low bicarbonate exchange activity. However, its participation in pyruvate metabolism in anaerobic dark-grown cells was not understood. During anaerobic, dark growth with pyruvate, formate was an intermediate in H2 and CO2 gas evolution. In contrast with H2 production by a light-dependent H2-nitrogenase system in photosynthetically grown cells, H2 formation in fermenting R. rubrum occurred through a carbon monoxide-sensitive formic hydrogenlyase reaction not influenced by light.

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Selected References

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  • Ariga N. Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones. Anal Biochem. 1972 Oct;49(2):436–441. [PubMed]
  • Bosshard-Heer E, Bachofen R. Synthese von Specicherstoffen aus Pyruvat durch Rhodospirillum rubrum. Arch Mikrobiol. 1969;65(1):61–75. [PubMed]
  • GEST H, ORMEROD JG, ORMEROD KS. Photometabolism of Rhodospirillum rubrum: light-dependent dissimilation of organic compounds to carbon dioxide and molecular hydrogen by an anaerobic citric acid cycle. Arch Biochem Biophys. 1962 Apr;97:21–33. [PubMed]
  • Good NE, Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53–68. [PubMed]
  • Hurlbert RE. Effect of oxygen on viability and substrate utilization in Chromatium. J Bacteriol. 1967 Apr;93(4):1346–1352. [PMC free article] [PubMed]
  • Keister DL, Yike NJ. Energy-linked reactions in photosynthetic bacteria. I. Succinatelinked ATP-driven NAD reduction by Rhodospirillum rubrum chromatophores. Arch Biochem Biophys. 1967 Aug;121(2):415–422. [PubMed]
  • Keister DL, Yike NJ. Energy-linked reactions in photosynthetic bacteria. II. The energy-dependent reduction of oxidized nicotinamide-adenine dinucleotide phosphate by reduced nicotinamide-adenine dinucleotide in chromatophores of Rhodospirillum rubrum. Biochemistry. 1967 Dec;6(12):3847–3857. [PubMed]
  • Knappe J, Blaschkowski HP, Gröbner P, Schmitt T. Pyruvate formate-lyase of Escherichia coli: the acetyl-enzyme intermediate. Eur J Biochem. 1974 Dec 16;50(1):253–263. [PubMed]
  • KOHLMILLER EF, Jr, GEST H. A comparative study of the light and dark fermentations of organic acids by Rhodo-spirillum rubrum. J Bacteriol. 1951 Mar;61(3):269–282. [PMC free article] [PubMed]
  • Li LF, Ljungdahl L, Wood HG. Properties of Nicotinamide Adenine Dinucleotide Phosphate-Dependent Formate Dehydrogenase from Clostridium thermoaceticum. J Bacteriol. 1966 Aug;92(2):405–412. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • McCormick NG, Ordal EJ, Whiteley HR. DEGRADATION OF PYRUVATE BY MICROCOCCUS LACTILYTICUS I. : General Properties of the Formate-Exchange Reaction. J Bacteriol. 1962 Apr;83(4):887–898. [PMC free article] [PubMed]
  • Nakayama H, Midwinter GG, Krampitz LO. Properties of the pyruvate formate-lyase reaction. Arch Biochem Biophys. 1971 Apr;143(2):526–534. [PubMed]
  • Raeburn S, Rabinowitz JC. Pyruvate: ferredoxin oxidoreductase. I. The pyruvate-CO 2 exchange reaction. Arch Biochem Biophys. 1971 Sep;146(1):9–20. [PubMed]
  • Reddy CA, Bryant MP, Wolin MJ. Ferredoxin- and nicotinamide adenine dinucleotide-dependent H 2 production from ethanol and formate in extracts of S organism isolated from "Methanobacillus omelianskii". J Bacteriol. 1972 Apr;110(1):126–132. [PMC free article] [PubMed]
  • Shanmugam KT, Buchanan BB, Arnon DI. Ferredoxins in light- and dark-grown photosynthetic cells with special reference to Rhodospirillum rubrum. Biochim Biophys Acta. 1972 Feb 28;256(2):477–486. [PubMed]
  • STADTMAN ER, BARKER HA. Fatty acid synthesis by enzyme preparations of Clostridium kluyveri. VI. Reactions of acyl phosphates. J Biol Chem. 1950 Jun;184(2):769–793. [PubMed]
  • Thauer RK, Fuchs G, Jungermann K. Reduced ferredoxin: CO2 oxidoreductase from Clostridium pasteurianum: its role in formate metabolism. J Bacteriol. 1974 May;118(2):758–760. [PMC free article] [PubMed]
  • Thauer RK, Kirchniawy FH, Jungermann KA. Properties and function of the pyruvate-formate-lyase reaction in clostridiae. Eur J Biochem. 1972 May 23;27(2):282–290. [PubMed]
  • Thauer RK, Rupprecht E, Jungermann K. Glyoxylate inhibition of clostridial pyruvate synthase. FEBS Lett. 1970 Aug 31;9(5):271–273. [PubMed]
  • Uffen RL. Growth properties of Rhodospirillum rubrum mutants and fermentation of pyruvate in anaerobic, dart conditions. J Bacteriol. 1973 Nov;116(2):874–884. [PMC free article] [PubMed]
  • Uffen RL, Sybesma C, Wolfe RS. Mutants of Rhodospirrillum rubrum obtained after long-term anaerobic, dark growth. J Bacteriol. 1971 Dec;108(3):1348–1356. [PMC free article] [PubMed]
  • Uffen RL, Wolfe RS. Anaerobic growth of purple nonsulfur bacteria under dark conditions. J Bacteriol. 1970 Oct;104(1):462–472. [PMC free article] [PubMed]
  • Wall JD, Weaver PF, Gest H. Genetic transfer of nitrogenase-hydrogenase activity in Rhodopseudomonas capsulata. Nature. 1975 Dec 18;258(5536):630–631. [PubMed]
  • Yoch DC, Lindstrom ES. Nicotinamide adenine dinucleotide-dependent formate dehydrogenase from Rhodopseudomonas palustris. Arch Mikrobiol. 1969;67(2):182–188. [PubMed]

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