Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1977 May; 130(2): 759–765.
PMCID: PMC235278

Analysis of plasmid deoxyribonucleic acid in a cariogenic strain of Streptococcus faecalis: an approach to identifying genetic determinants on cryptic plasmids.


Streptococcus faecalis strains ND539 and OG1 have been previously shown to be cariogenic in gnotobiotic animals. Deoxyribonucleic acid analyses have revealed the presence of a single 26-megadalton plasmid designated pAM539 in the former strain, whereas the latter strain was found to be plasmid-free. By gene transfer experiments, it was possible to construct isogenic pairs of strains that differed only with regard to the presence or absence of pAM539. Comparative studies of isogenic pairs showed that the presence of pAM539 conferred bacterial sensitivity to a bacteriocin produced by S. faecalis strain 5952.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bak AL, Christiansen C, Stenderup A. Bacterial genome sizes determined by DNA renaturation studies. J Gen Microbiol. 1970 Dec;64(3):377–380. [PubMed]
  • Bazaral M, Helinski DR. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513–3520. [PubMed]
  • Clewell DB, Helinski DR. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. [PubMed]
  • Clewell DB, Yagi Y, Bauer B. Plasmid-determined tetracycline resistance in Streptococcus faecalis: evidence for gene amplification during growth in presence of tetracycline. Proc Natl Acad Sci U S A. 1975 May;72(5):1720–1724. [PMC free article] [PubMed]
  • Clewell DB, Yagi Y, Dunny GM, Schultz SK. Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J Bacteriol. 1974 Jan;117(1):283–289. [PMC free article] [PubMed]
  • Clowes RC. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. [PMC free article] [PubMed]
  • Dunny GM, Birch N, Hascall G, Clewell DB. Isolation and characterization of plasmid deoxyribonucleic acid from Streptococcus mutans. J Bacteriol. 1973 Jun;114(3):1362–1364. [PMC free article] [PubMed]
  • Dunny GM, Clewell DB. Transmissible toxin (hemolysin) plasmid in Streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid. J Bacteriol. 1975 Nov;124(2):784–790. [PMC free article] [PubMed]
  • Gold OG, Jordan HV, van Houte J. The prevalence of enterococci in the human mouth and their pathogenicity in animal models. Arch Oral Biol. 1975 Jul;20(7):473–477. [PubMed]
  • Helinski DR, Clewell DB. Circular DNA. Annu Rev Biochem. 1971;40:899–942. [PubMed]
  • Higuchi M, Araya S, Higuchi M. Plasmid DNA satellite bands seen in lysates of Streptococcus mutans that form insoluble extracellular polysaccharides. J Dent Res. 1976 Mar-Apr;55(2):266–271. [PubMed]
  • Howarth S. Resistance to the bactericidal effect of ultraviolet radiation conferred on Enterobacteria by the colicine factor coli. J Gen Microbiol. 1965 Jul;40(1):43–55. [PubMed]
  • Jacob AE, Hobbs SJ. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. [PMC free article] [PubMed]
  • MacPhee DG. Effects of an R factor and caffeine on ultraviolet mutability in Salmonella typhimurium. Mutat Res. 1973 Jun;18(3):367–370. [PubMed]
  • Novick RP, Roth C. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J Bacteriol. 1968 Apr;95(4):1335–1342. [PMC free article] [PubMed]
  • ORLAND FJ, BLAYNEY JR, HARRISON RW, REYNIERS JA, TREXLER PC, ERVIN RF, GORDON HA, WAGNER M. Experimental caries in germfree rats inoculated with enterococci. J Am Dent Assoc. 1955 Mar;50(3):259–272. [PubMed]
  • Tomura T, Hirano T, Ito T, Yoshioka M. Transmission of bacteriocinogenicity by conjugation in group D streptococci. Jpn J Microbiol. 1973 Nov;17(6):445–452. [PubMed]
  • Watson B, Currier TC, Gordon MP, Chilton MD, Nester EW. Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol. 1975 Jul;123(1):255–264. [PMC free article] [PubMed]
  • WILLIAMS NB, FORBES MA, BLAU E, EICKENBERG CF. A study of the simultaneous occurrence of Enterococci, Lactobacilli, and yeasts in saliva from human beings. J Dent Res. 1950 Oct;29(5):563–570. [PubMed]
  • WINKLER KC, VAN AMERONGEN J. Bacteriologic results from 4,000 root canal cultures. Oral Surg Oral Med Oral Pathol. 1959 Jul;12(7):857–875. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...