• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Feb 1977; 129(2): 651–657.
PMCID: PMC234994

Accumulation of nucleotides by starved Escherichia coli cells as a probe for the involvement of ribonucleases in ribonucleic acid degradation.

Abstract

The acid-soluble ribonucleic acid degradation products formed by Escherichia coli cells starved for a carbon source have been identified. They comprise oligonucleotides, nucleoside diphosphates, 5'- and 3'-nucleoside monophosphates, nucleosides, and free bases. The majority of these products are excreted phates, nucleosides, and free bases. The majority of these products are excreted into the medium, and only small and constant amounts are kept in the pool. During carbon starvation at elevated temperatures, mutants deficient in ribonuclease I do not form oligonucleotides and 3'-nucleoside monophosphates, and mutants that contain a modified form of polynucleotide phosphorylase do not accumulate nucleoside diphosphates. 5'-Nucleoside monophosphates do accumulate, however, in a mutant containing thermoabile ribonuclease II, under conditions where more than 95% of all enzyme activity had been destroyed. The data presented confirm the participation of ribonuclease I and polynucleotide phosphorylase in the final steps of ribonucleic acid degradation and indicate that an exonuclease forming 5'-nucleoside monophosphates is also involved.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anderson EH. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. [PMC free article] [PubMed]
  • Barnard EA. Ribonucleases. Annu Rev Biochem. 1969;38:677–732. [PubMed]
  • Cohen L, Kaplan R. The resolution of pyrimidine bases, nucleosides, and nucleotides by thin-layer chromatography. Anal Biochem. 1975 Nov;69(1):283–288. [PubMed]
  • Datta AK, Niyogi K. A novel oligoribonuclease of Escherichia coli. II. Mechanism of action. J Biol Chem. 1975 Sep 25;250(18):7313–7319. [PubMed]
  • FURLONG NB. A SIMPLE AND RAPID METHOD FOR DETERMINING THE CHAIN LENGTH OF OLIGONUCLEOTIDES RANDOMLY LABELED WITH P32. Anal Biochem. 1965 Aug;12:349–356. [PubMed]
  • Guissani A. Properties of polynucleotide phosphorylase from E. coli Polynucleotide phosphorylase-deficient and thermo-sensitive mutants. FEBS Lett. 1975 Jul 1;54(3):303–306. [PubMed]
  • Kaplan R, Apirion D. The involvement of ribonuclease I, ribonuclease II, and polynucleotide phosphorylase in the degradation of stable ribonucleic acid during carbon starvation in Escherichia coli. J Biol Chem. 1974 Jan 10;249(1):149–151. [PubMed]
  • Kaplan R, Apirion D. Decay of ribosomal ribonucleic acid in Escherichia coli cells starved for various nutrients. J Biol Chem. 1975 Apr 25;250(8):3174–3178. [PubMed]
  • Kaplan R, Apirion D. The fate of ribosomes in Escherichia coli cells starved for a carbon source. J Biol Chem. 1975 Mar 10;250(5):1854–1863. [PubMed]
  • Kaplan R, Cohen L, Yagil E. Acid-soluble degradation products of ribonucleic acid in Escherichia coli and the role of nucleotidases in their catabolism. J Bacteriol. 1975 Dec;124(3):1159–1164. [PMC free article] [PubMed]
  • Kaplan R, Hartstein E. The site of ribosome degradation in starved Escherichia coli cells. J Biol Chem. 1976 Feb 25;251(4):1147–1153. [PubMed]
  • Klee CB, Singer MF. The processive degradation of individual polyribonucleotide chains. II. Micrococcus lysodeikticus polynucleotide phosphorylase. J Biol Chem. 1968 Mar 10;243(5):923–927. [PubMed]
  • LICHTENSTEIN J, BARNER HD, COHEN SS. The metabolism of exogenously supplied nucleotides by Escherichia coli. J Biol Chem. 1960 Feb;235:457–465. [PubMed]
  • Maruyama H, Mizuno D. Ribosome degradation and the degradation products in starved Escherichia coli. I. Comparison of the degradation rate and of the nucleotide pool between Escherichia coli B and Q-13 strains in phosphate deficiency. Biochim Biophys Acta. 1970 Jan 21;199(1):159–165. [PubMed]
  • Maruyama H, Ono-Onitsuka M, Mizuno D. Ribosomal RNA degradation and the degradation products in starved Escherichia coli. IV. The cellular components excreted into the media during different deprivation conditions. J Biochem. 1970 Apr;67(4):559–565. [PubMed]
  • NEU HC, HEPPEL LA. THE RELEASE OF RIBONUCLEASE INTO THE MEDIUM WHEN ESCHERICHIA COLI CELLS ARE CONVERTED TO SPEROPLASTS. J Biol Chem. 1964 Nov;239:3893–3900. [PubMed]
  • Niyogi SK, Datta AK. A novel oligoribonuclease of Escherichia coli. I. Isolation and properties. J Biol Chem. 1975 Sep 25;250(18):7307–7312. [PubMed]
  • Nossal NG, Singer MF. The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II. J Biol Chem. 1968 Mar 10;243(5):913–922. [PubMed]
  • Nozawa R, Horiuchi T, Mizuno D. Degradation of ribosomal RNA in a temperature-sensitive Escherichia coli. Arch Biochem Biophys. 1967 Feb;118(2):402–409. [PubMed]
  • O'Donovan GA, Neuhard J. Pyrimidine metabolism in microorganisms. Bacteriol Rev. 1970 Sep;34(3):278–343. [PMC free article] [PubMed]
  • Piérard A, Glansdorff N, Yashphe J. Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake. Mol Gen Genet. 1972;118(3):235–245. [PubMed]
  • Reiner AM. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli. J Bacteriol. 1969 Mar;97(3):1431–1436. [PMC free article] [PubMed]
  • Weatherford SC, Rosen L, Gorelic L, Apirion D. Escherichia coli strains with thermolabile ribonuclease II activity. J Biol Chem. 1972 Sep 10;247(17):5404–5408. [PubMed]
  • Yagil E, Beacham IR. Uptake of adenosine 5'-monophosphate by Escherichia coli. J Bacteriol. 1975 Feb;121(2):401–405. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...