• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jan 1977; 129(1): 237–245.
PMCID: PMC234920

Mutations in Prophage [var phi]11 That Impair the Transducibility of Their Staphylococcus aureus Lysogens for Methicillin Resistance

Abstract

Methicillin resistance (mec) is not transduced into Staphylococcus aureus 8325-4, but is transduced into this host after it has been lysogenized with phage [var phi]11 and has acquired the penicillinase plasmid pI524 by a separate transduction (Cohen and Sweeney, 1970, 1973). Strain 8325-4 is competent for transformation of typical plasmid or chromosomal markers and for mec only if it is lysogenic for [var phi]11 or a related prophage (Sjöström et al., 1974, 1975). A mutant strain of [var phi]11 that was temperature sensitive (Ts) for vegetative multiplication did not mediate competence for transformation of its 8325-4 lysogen if the lysogen had been grown at a nonpermissive temperature (Sjöström and Philipson, 1974). We isolated four Ts mutants of [var phi]11 that did not mediate transducibility of their 8325-4(pI524) lysogens for mec after growth at nonpermissive temperatures (40 to 42°C). Transduction of typical plasmid or chromosomal markers was not affected. These [var phi]11-Ts mutants mediated normal competence of their lysogens for transformation of a tetracycline resistance plasmid. Similarly, [var phi]11-Ts mutants that rendered their lysogens temperature sensitive for transformation did not depress the frequency of transduction of mec. These two types of [var phi]11-Ts mutants fell into two different genetic complementation groups that differed in the physiology of deoxyribonucleic acid synthesis and in the time of expression of the mutations during a single-burst growth cycle at a nonpermissive temperature. A virulent mutant of [var phi]11, which plaqued with 100% efficiency on 8325([var phi]11), also failed to condition strain 8325-4 for transducibility of mec but retained the ability to confer competence for transformation of a tetracycline resistance plasmid. Different genetic loci and physiological functions are involved in [var phi]11 mutations that affect transducibility of mec and those that affect competence for transformation of markers generally in S. aureus 8325-4.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Botstein D, Chan RK, Waddell CH. Genetics of bacteriophage P22. II. Gene order and gene function. Virology. 1972 Jul;49(1):268–282. [PubMed]
  • Cohen S, Sweeney HM. Transduction of Methicillin Resistance in Staphylococcus aureus Dependent on an Unusual Specificity of the Recipient Strain. J Bacteriol. 1970 Dec;104(3):1158–1167. [PMC free article] [PubMed]
  • Cohen S, Sweeney HM. Effect of the prophage and penicillinase plasmid of the recipient strain upon the transduction and the stability of methicillin resistance in Staphylococcus aureus. J Bacteriol. 1973 Nov;116(2):803–811. [PMC free article] [PubMed]
  • Dornbusch K, Hallander HO, Löfquist F. Extrachromosomal control of methicillin resistance and toxin production in Staphylococcus aureus. J Bacteriol. 1969 May;98(2):351–358. [PMC free article] [PubMed]
  • Ebel-Tsipis J, Fox MS, Botstein D. Generalized transduction by bacteriophage P22 in Salmonella typhimurium. II. Mechanism of integration of transducing DNA. J Mol Biol. 1972 Nov 14;71(2):449–469. [PubMed]
  • Kretschmer PJ, Egan JB. Isolation of a suppressor host bacterium in Staphylococcus aureus. J Bacteriol. 1973 Oct;116(1):84–87. [PMC free article] [PubMed]
  • Kretschmer PJ, Egan JB. Genetic map of the Staphylococcal bacteriophage phi11. J Virol. 1975 Sep;16(3):642–651. [PMC free article] [PubMed]
  • Lacey RW, Grinsted J. Genetic analysis of methicillin-resistant strains of Staphylococcus aureus; evidence for their evolution from a single clone. J Med Microbiol. 1973 Nov;6(4):511–526. [PubMed]
  • Notani NK, Setlow JK. Mechanism of bacterial transformation and transfection. Prog Nucleic Acid Res Mol Biol. 1974;14(0):39–100. [PubMed]
  • NOVICK RP. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. [PubMed]
  • Novick R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967 Sep;33(1):155–166. [PubMed]
  • Novick RP, Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. [PubMed]
  • Schwesinger MD, Novick RP. Prophage-dependent plasmid integration in Staphylococcus aureus. J Bacteriol. 1975 Aug;123(2):724–738. [PMC free article] [PubMed]
  • Sjöström JE, Philipson L. Role of the phi 11 phage genome in competence of Staphylococcus aureus. J Bacteriol. 1974 Jul;119(1):19–32. [PMC free article] [PubMed]
  • Sjöström JE, Löfdahl S, Philipson L. Transformation reveals a chromosomal locus of the gene(s) for methicillin resistance in Staphylococcus aureus. J Bacteriol. 1975 Sep;123(3):905–915. [PMC free article] [PubMed]
  • Stiffler PW, Sweeney HM, Cohen S. Co-transduction of plasmids mediating resistance to tetracycline and chloramphenicol in Staphylococcus aureus. J Bacteriol. 1974 Nov;120(2):934–934. [PMC free article] [PubMed]
  • Studier FW. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. [PubMed]
  • Wyman L, Goering RV, Novick RP. Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics. 1974 Apr;76(4):681–702. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...