• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Dec 1976; 128(3): 785–793.
PMCID: PMC232769

Fosfomycin resistance: selection method for internal and extended deletions of the phosphoenolpyruvate:sugar phosphotransferase genes of Salmonella typhimurium.


Selection for resistance to the antibiotic fosfomycin (FOS; L-cis 1,2-epoxypropylphosphonic acid, a structural analogue of phosphoenolpyruvate) was used to isolate mutants carrying internal and extended deletions of varying lengths within the ptsHI operon of Salmonella typhimurium. Strains carrying "tight" ptsI point mutations and all mutants in which some or all of the ptsI gene was deleted were FOS resistant. In contrast, strains carrying ptsH point mutations were sensitive to FOS. Resistance to FOS appeared to result indirectly from catabolite repression of an FOS transport system, probably the sn-glycerol-3-phosphate transport system. Resistant ptsI mutants became sensitive to FOS when grown on D-glucose-6-phosphate, which induces an alternate transport system for FOS, or when grown in the presence of cyclic adenosine 3',5'-monophosphate. A detailed fine-structure map of the pts gene region is presented.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Alper MD, Ames BN. Cyclic 3', 5'-adenosine monophosphate phosphodiesterase mutants of Salmonella typhimurium. J Bacteriol. 1975 Jun;122(3):1081–1090. [PMC free article] [PubMed]
  • Anderson B, Weigel N, Kundig W, Roseman S. Sugar transport. 3. Purification and properties of a phosphocarrier protein (HPr) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli. J Biol Chem. 1971 Nov 25;246(22):7023–7033. [PubMed]
  • Boro H, Brenchley JE. A new generalized transducing phage for Salmonella typhimurium LT2. Virology. 1971 Sep;45(3):835–836. [PubMed]
  • Brickman E, Soll L, Beckwith J. Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. J Bacteriol. 1973 Nov;116(2):582–587. [PMC free article] [PubMed]
  • Cordaro JC, Roseman S. Deletion mapping of the genes coding for HPr and enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system in Salmonella typhimurium. J Bacteriol. 1972 Oct;112(1):17–29. [PMC free article] [PubMed]
  • DAVIS BD, MINGIOLI ES. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. [PMC free article] [PubMed]
  • Kadner RJ, Winkler HH. Isolation and characterization of mutations affecting the transport of hexose phosphates in Escherichia coli. J Bacteriol. 1973 Feb;113(2):895–900. [PMC free article] [PubMed]
  • Kahan FM, Kahan JS, Cassidy PJ, Kropp H. The mechanism of action of fosfomycin (phosphonomycin). Ann N Y Acad Sci. 1974 May 10;235(0):364–386. [PubMed]
  • Kundig W. Molecular interactions in the bacterial phosphoenolpyruvate-phosphotransferase system (PTS). J Supramol Struct. 1974;2(5-6):695–814. [PubMed]
  • Kundig W, Roseman S. Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli. J Biol Chem. 1971 Mar 10;246(5):1393–1406. [PubMed]
  • Kundig W, Roseman S. Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the Escherichia coli phosphotransferase system. J Biol Chem. 1971 Mar 10;246(5):1407–1418. [PubMed]
  • MAKMAN RS, SUTHERLAND EW. ADENOSINE 3',5'-PHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1965 Mar;240:1309–1314. [PubMed]
  • Melton T, Kundig W, Hartman PE, Meadow N. 3-Deoxy-3-fluoro-D-glucose-resistant Salmonella typhimurium mutants defective in the phosphoenolpyruvate:glycose phosphotransferase system. J Bacteriol. 1976 Dec;128(3):794–800. [PMC free article] [PubMed]
  • Pastan I, Perlman R. Cyclic adenosine monophosphate in bacteria. Science. 1970 Jul 24;169(3943):339–344. [PubMed]
  • Peterkofsky A, Gazdar C. Interaction of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system with adenylate cyclase of Escherichia coli. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2920–2924. [PMC free article] [PubMed]
  • Saier MH, Jr, Feucht BU, McCaman MT. Regulation of intracellular adenosine cyclic 3':5'-monophosphate levels in Escherichia coli and Salmonella typhimurium. Evidence for energy-dependent excretion of the cyclic nucleotide. J Biol Chem. 1975 Oct 10;250(19):7593–7601. [PubMed]
  • Saier MH, Jr, Simoni RD, Roseman S. The physiological behavior of enzyme I and heat-stable protein mutants of a bacterial phosphotransferase system. J Biol Chem. 1970 Nov 10;245(21):5870–5873. [PubMed]
  • Tao M, Lipmann F. Isolation of adenyl cyclase from Escherichia coli. Proc Natl Acad Sci U S A. 1969 May;63(1):86–92. [PMC free article] [PubMed]
  • Venkateswaran PS, Wu HC. Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12. J Bacteriol. 1972 Jun;110(3):935–944. [PMC free article] [PubMed]
  • Wu HC, Venkateswaran PS. Fosfomycin-resistant mutant of Escherichia coli. Ann N Y Acad Sci. 1974 May 10;235(0):587–592. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...