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Mol Cell Biol. Jan 1995; 15(1): 87–93.
PMCID: PMC231911

A selector of transcription initiation in the protozoan parasite Toxoplasma gondii.

Abstract

The recent development of an efficient transfection system for the apicomplexan Toxoplasma gondii allows a comprehensive dissection of the elements involved in gene transcription in this obligate intracellular parasite. We demonstrate here that for the SAG1 gene, a stretch of six repeated sequences in the region 35 to 190 bp upstream of the first of two transcription start sites is essential for efficient and accurate transcription initiation. This repeat element shows characteristics of a selector in determining the position of the transcription start sites.

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Selected References

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  • Bangs JD, Crain PF, Hashizume T, McCloskey JA, Boothroyd JC. Mass spectrometry of mRNA cap 4 from trypanosomatids reveals two novel nucleosides. J Biol Chem. 1992 May 15;267(14):9805–9815. [PubMed]
  • Ben Amar MF, Jefferies D, Pays A, Bakalara N, Kendall G, Pays E. The actin gene promoter of Trypanosoma brucei. Nucleic Acids Res. 1991 Nov 11;19(21):5857–5862. [PMC free article] [PubMed]
  • Blake MC, Jambou RC, Swick AG, Kahn JW, Azizkhan JC. Transcriptional initiation is controlled by upstream GC-box interactions in a TATAA-less promoter. Mol Cell Biol. 1990 Dec;10(12):6632–6641. [PMC free article] [PubMed]
  • Bülow R, Boothroyd JC. Protection of mice from fatal Toxoplasma gondii infection by immunization with p30 antigen in liposomes. J Immunol. 1991 Nov 15;147(10):3496–3500. [PubMed]
  • Burg JL, Perelman D, Kasper LH, Ware PL, Boothroyd JC. Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J Immunol. 1988 Nov 15;141(10):3584–3591. [PubMed]
  • Clayton CE, Fueri JP, Itzhaki JE, Bellofatto V, Sherman DR, Wisdom GS, Vijayasarathy S, Mowatt MR. Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei. Mol Cell Biol. 1990 Jun;10(6):3036–3047. [PMC free article] [PubMed]
  • Cross GA. Cellular and genetic aspects of antigenic variation in trypanosomes. Annu Rev Immunol. 1990;8:83–110. [PubMed]
  • Dubremetz JF, Rodriguez C, Ferreira E. Toxoplasma gondii: redistribution of monoclonal antibodies on tachyzoites during host cell invasion. Exp Parasitol. 1985 Feb;59(1):24–32. [PubMed]
  • Grosschedl R, Birnstiel ML. Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102–7106. [PMC free article] [PubMed]
  • Kasper LH. Identification of stage-specific antigens of Toxoplasma gondii. Infect Immun. 1989 Mar;57(3):668–672. [PMC free article] [PubMed]
  • Kasper LH, Bradley MS, Pfefferkorn ER. Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J Immunol. 1984 Jan;132(1):443–449. [PubMed]
  • Kasper LH, Crabb JH, Pfefferkorn ER. Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody. J Immunol. 1983 May;130(5):2407–2412. [PubMed]
  • Khan IA, Ely KH, Kasper LH. A purified parasite antigen (p30) mediates CD8+ T cell immunity against fatal Toxoplasma gondii infection in mice. J Immunol. 1991 Nov 15;147(10):3501–3506. [PubMed]
  • Kim K, Soldati D, Boothroyd JC. Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. Science. 1993 Nov 5;262(5135):911–914. [PubMed]
  • Kollmar R, Farnham PJ. Site-specific initiation of transcription by RNA polymerase II. Proc Soc Exp Biol Med. 1993 Jun;203(2):127–139. [PubMed]
  • McAndrew MB, Read M, Sims PF, Hyde JE. Characterisation of the gene encoding an unusually divergent TATA-binding protein (TBP) from the extremely A+T-rich human malaria parasite Plasmodium falciparum. Gene. 1993 Feb 28;124(2):165–171. [PubMed]
  • Mineo JR, McLeod R, Mack D, Smith J, Khan IA, Ely KH, Kasper LH. Antibodies to Toxoplasma gondii major surface protein (SAG-1, P30) inhibit infection of host cells and are produced in murine intestine after peroral infection. J Immunol. 1993 May 1;150(9):3951–3964. [PubMed]
  • Nagel SD, Boothroyd JC. The alpha- and beta-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple introns. Mol Biochem Parasitol. 1988 Jun;29(2-3):261–273. [PubMed]
  • Pavé-Preux M, Aggerbeck M, Veyssier C, Bousquet-Lemercier B, Hanoune J, Barouki R. Hormonal discrimination among transcription start sites of aspartate aminotransferase. J Biol Chem. 1990 Mar 15;265(8):4444–4448. [PubMed]
  • Pays E, Coquelet H, Tebabi P, Pays A, Jefferies D, Steinert M, Koenig E, Williams RO, Roditi I. Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters. EMBO J. 1990 Oct;9(10):3145–3151. [PMC free article] [PubMed]
  • Schatt MD, Rusconi S, Schaffner W. A single DNA-binding transcription factor is sufficient for activation from a distant enhancer and/or from a promoter position. EMBO J. 1990 Feb;9(2):481–487. [PMC free article] [PubMed]
  • Smale ST, Baltimore D. The "initiator" as a transcription control element. Cell. 1989 Apr 7;57(1):103–113. [PubMed]
  • Soldati D, Boothroyd JC. Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii. Science. 1993 Apr 16;260(5106):349–352. [PubMed]
  • Tomavo S, Fortier B, Soete M, Ansel C, Camus D, Dubremetz JF. Characterization of bradyzoite-specific antigens of Toxoplasma gondii. Infect Immun. 1991 Oct;59(10):3750–3753. [PMC free article] [PubMed]
  • van den Hoff MJ, Moorman AF, Lamers WH. Electroporation in 'intracellular' buffer increases cell survival. Nucleic Acids Res. 1992 Jun 11;20(11):2902–2902. [PMC free article] [PubMed]
  • White RJ, Jackson SP. The TATA-binding protein: a central role in transcription by RNA polymerases I, II and III. Trends Genet. 1992 Aug;8(8):284–288. [PubMed]
  • Wieland S, Schatt MD, Rusconi S. Role of TATA-element in transcription from glucocorticoid receptor-responsive model promoters. Nucleic Acids Res. 1990 Sep 11;18(17):5113–5118. [PMC free article] [PubMed]
  • Zomerdijk JC, Ouellette M, ten Asbroek AL, Kieft R, Bommer AM, Clayton CE, Borst P. The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei. EMBO J. 1990 Sep;9(9):2791–2801. [PMC free article] [PubMed]

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