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J Clin Microbiol. Dec 1997; 35(12): 3203–3209.
PMCID: PMC230148

A new agent of mycobacterial lymphadenitis in children: Mycobacterium heidelbergense sp. nov.

Abstract

Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differentiated biochemically from described Mycobacterium species, although it most closely resembled Mycobacterium malmoense by thin-layer chromatography and high-performance liquid chromatography of mycolic acids. A striking characteristic of the isolate was its high degree of susceptibility to antituberculous drugs in vitro, including isoniazid. Direct determination of the 16S rRNA gene sequence revealed a unique sequence and positioned the strain phylogenetically on a branch separate from M. malmoense within a group of slowly growing mycobacteria that show a high degree of similarity to M. simiae at the 16S rRNA gene level. Despite 99.6% sequence identity with M. simiae at the 16S rRNA gene level, DNA-DNA hybridization studies (hydroxyapatite method) demonstrated DNA relatedness of less than 40%. We conclude that this organism is a new species for which we propose the name M. heidelbergense. A culture of the type strain, strain 2554/91, has been deposited in the American Type Culture Collection as strain ATCC 51253.

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Selected References

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  • Böddinghaus B, Wolters J, Heikens W, Böttger EC. Phylogenetic analysis and identification of different serovars of Mycobacterium intracellulare at the molecular level. FEMS Microbiol Lett. 1990 Jul;58(2):197–203. [PubMed]
  • Brenner DJ, McWhorter AC, Knutson JK, Steigerwalt AG. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol. 1982 Jun;15(6):1133–1140. [PMC free article] [PubMed]
  • Butler WR, Thibert L, Kilburn JO. Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol. 1992 Oct;30(10):2698–2704. [PMC free article] [PubMed]
  • Edwards U, Rogall T, Blöcker H, Emde M, Böttger EC. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 1989 Oct 11;17(19):7843–7853. [PMC free article] [PubMed]
  • Falkinham JO., 3rd Epidemiology of infection by nontuberculous mycobacteria. Clin Microbiol Rev. 1996 Apr;9(2):177–215. [PMC free article] [PubMed]
  • Fitzpatrick EL, LeJeune FE., Jr Mycobacterial cervical lymphadenitis: a review. J La State Med Soc. 1996 Nov;148(11):451–454. [PubMed]
  • Floyd MM, Guthertz LS, Silcox VA, Duffey PS, Jang Y, Desmond EP, Crawford JT, Butler WR. Characterization of an SAV organism and proposal of Mycobacterium triplex sp. nov. J Clin Microbiol. 1996 Dec;34(12):2963–2967. [PMC free article] [PubMed]
  • Haas WH, Kirschner P, Ziesing S, Bremer HJ, Böttger EC. Cervical lymphadenitis in a child caused by a previously unknown mycobacterium. J Infect Dis. 1993 Jan;167(1):237–240. [PubMed]
  • Kuth G, Lamprecht J, Haase G. Cervical lymphadenitis due to mycobacteria other than tuberculosis--an emerging problem in children? ORL J Otorhinolaryngol Relat Spec. 1995 Jan-Feb;57(1):36–38. [PubMed]
  • Lamden K, Watson JM, Knerer G, Ryan MJ, Jenkins PA. Opportunist mycobacteria in England and Wales: 1982 to 1994. Commun Dis Rep CDR Rev. 1996 Oct 11;6(11):R147–R151. [PubMed]
  • Lévy-Frébault V, Goh KS, David HL. Mycolic acid analysis for clinical identification of Mycobacterium avium and related mycobacteria. J Clin Microbiol. 1986 Nov;24(5):835–839. [PMC free article] [PubMed]
  • Liberek V, Soravia C, Ninet B, Hirschel B, Siegrist CA. Cervical lymphadenitis caused by Mycobacterium genavense in a healthy child. Pediatr Infect Dis J. 1996 Mar;15(3):269–270. [PubMed]
  • Meissner G, Schröder KH. Relationship between Mycobacterium simiae and Mycobacterium habana. Am Rev Respir Dis. 1975 Feb;111(2):196–200. [PubMed]
  • Plikaytis BB, Plikaytis BD, Yakrus MA, Butler WR, Woodley CL, Silcox VA, Shinnick TM. Differentiation of slowly growing Mycobacterium species, including Mycobacterium tuberculosis, by gene amplification and restriction fragment length polymorphism analysis. J Clin Microbiol. 1992 Jul;30(7):1815–1822. [PMC free article] [PubMed]
  • PRISSICK FH, MASSON AM. Yellow-pigmented pathogenic mycobacteria from cervical lymphadenitis. Can J Microbiol. 1957 Feb;3(1):91–100. [PubMed]
  • Rogall T, Flohr T, Böttger EC. Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol. 1990 Sep;136(9):1915–1920. [PubMed]
  • Rogall T, Wolters J, Flohr T, Böttger EC. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol. 1990 Oct;40(4):323–330. [PubMed]
  • Schaad UB, Votteler TP, McCracken GH, Jr, Nelson JD. Management of atypical mycobacterial lymphadenitis in childhood: a review based on 380 cases. J Pediatr. 1979 Sep;95(3):356–360. [PubMed]
  • Smid I, Salfinger M. Mycobacterial identification by computer-aided gas-liquid chromatography. Diagn Microbiol Infect Dis. 1994 Jun;19(2):81–88. [PubMed]
  • Springer B, Wu WK, Bodmer T, Haase G, Pfyffer GE, Kroppenstedt RM, Schröder KH, Emler S, Kilburn JO, Kirschner P, et al. Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov. J Clin Microbiol. 1996 May;34(5):1100–1107. [PMC free article] [PubMed]
  • Stahl DA, Urbance JW. The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol. 1990 Jan;172(1):116–124. [PMC free article] [PubMed]
  • Teske A, Wolters J, Böttger EC. The 16S rRNA nucleotide sequence of Mycobacterium leprae: phylogenetic position and development of DNA probes. FEMS Microbiol Lett. 1991 May 15;64(2-3):231–237. [PubMed]
  • Tortoli E, Bartoloni A, Burrini C, Mantella A, Simonetti MT. Utility of high-performance liquid chromatography for identification of mycobacterial species rarely encountered in clinical laboratories. Eur J Clin Microbiol Infect Dis. 1995 Mar;14(3):240–243. [PubMed]
  • Wright JE. Non-tuberculous mycobacterial lymphadenitis. Aust N Z J Surg. 1996 Apr;66(4):225–228. [PubMed]

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