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J Clin Microbiol. Dec 1997; 35(12): 3150–3155.
PMCID: PMC230139

Emergence of a unique O3:K6 clone of Vibrio parahaemolyticus in Calcutta, India, and isolation of strains from the same clonal group from Southeast Asian travelers arriving in Japan.

Abstract

Active surveillance of Vibrio parahaemolyticus infection among hospitalized patients in Calcutta, India, was initiated in January 1994. The incidence of cases of V. parahaemolyticus infection suddenly increased in February 1996 and has remained high since then. One hundred thirty-four strains of V. parahaemolyticus isolated from January 1994 to August 1996 were examined for serovar, the presence of the thermostable direct hemolysin gene (tdh) and tdh-related hemolysin genes (trh1 and trh2), production of urease, and antibiogram. Strains of the O3:K6 serovar appeared for the first time in February 1996. The O3:K6 serovar strains accounted for 50 to 80% of the strains isolated during the high-incidence period (February to August 1996). All of the serovar O3:K6 strains carried the tdh gene but not the trh genes and did not produce urease. All of the isolates except two were sensitive to all of the antibiotics tested. These and the results of analysis by an arbitrarily primed PCR method indicated that the O3:K6 serovar strains belong to a unique clone. When the O3:K6 serovar strains, isolated from travelers arriving in Japan from Southeast Asian countries, were compared by the arbitrarily primed PCR method, the strains isolated between 1982 and 1993 were distinct from Calcutta O3:K6 while the strains isolated in 1995 and 1996 were indistinguishable from the Calcutta O3:K6 strains. The results suggest that this unique O3:K6 clone may have become prevalent not only in Calcutta but also in Southeast Asian countries very recently. Not only the O3:K6 strains but also the non-O3:K6, tdh-bearing strains isolated in 1996 produced thermostable direct hemolysin at high levels, and thus the level of hemolysin produced does not appear to have influenced the high incidence of serovar O3:K6 strains.

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Selected References

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  • Abbott SL, Powers C, Kaysner CA, Takeda Y, Ishibashi M, Joseph SW, Janda JM. Emergence of a restricted bioserovar of Vibrio parahaemolyticus as the predominant cause of Vibrio-associated gastroenteritis on the West Coast of the United States and Mexico. J Clin Microbiol. 1989 Dec;27(12):2891–2893. [PMC free article] [PubMed]
  • Cai Y, Ni Y. Purification, characterization, and pathogenicity of urease produced by Vibrio parahaemolyticus. J Clin Lab Anal. 1996;10(2):70–73. [PubMed]
  • Hondo S, Goto I, Minematsu I, Ikeda N, Asano N, Ishibashi M, Kinoshita Y, Nishibuchi N, Honda T, Miwatani T. Gastroenteritis due to Kanagawa negative Vibrio parahaemolyticus. Lancet. 1987 Feb 7;1(8528):331–332. [PubMed]
  • Honda T, Ni YX, Miwatani T. Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin. Infect Immun. 1988 Apr;56(4):961–965. [PMC free article] [PubMed]
  • Huq MI, Huber D, Kibryia G. Isolation of urease producing Vibrio parahaemolyticus strains from cases of gastroenteritis. Indian J Med Res. 1979 Oct;70:549–553. [PubMed]
  • Janda JM, Powers C, Bryant RG, Abbott SL. Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin Microbiol Rev. 1988 Jul;1(3):245–267. [PMC free article] [PubMed]
  • Kelly MT, Stroh EM. Urease-positive, Kanagawa-negative Vibrio parahaemolyticus from patients and the environment in the Pacific Northwest. J Clin Microbiol. 1989 Dec;27(12):2820–2822. [PMC free article] [PubMed]
  • Kishishita M, Matsuoka N, Kumagai K, Yamasaki S, Takeda Y, Nishibuchi M. Sequence variation in the thermostable direct hemolysin-related hemolysin (trh) gene of Vibrio parahaemolyticus. Appl Environ Microbiol. 1992 Aug;58(8):2449–2457. [PMC free article] [PubMed]
  • Lin Z, Kumagai K, Baba K, Mekalanos JJ, Nishibuchi M. Vibrio parahaemolyticus has a homolog of the Vibrio cholerae toxRS operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. J Bacteriol. 1993 Jun;175(12):3844–3855. [PMC free article] [PubMed]
  • Magalhães M, Takeda Y, Magalhães V, Tateno S. Brazilian urease-positive strains of Vibrio parahaemolyticus carry genetic potential to produce the TDH-related hemolysin. Mem Inst Oswaldo Cruz. 1992 Jan-Mar;87(1):167–168. [PubMed]
  • Magalhães V, Lima RA, Tateno S, Magalhães M. Gastroenteritis humanas associadas a Vibrio parahaemolyticus no Recife, Brasil. Rev Inst Med Trop Sao Paulo. 1991 Jan-Feb;33(1):64–68. [PubMed]
  • MASLEN LGC. Routine use of liquid urea medium for identifying Salmonella and Shigella organisms. Br Med J. 1952 Sep 6;2(4783):545–546. [PMC free article] [PubMed]
  • Minami A, Hashimoto S, Abe H, Arita M, Taniguchi T, Honda T, Miwatani T, Nishibuchi M. Cholera enterotoxin production in Vibrio cholerae O1 strains isolated from the environment and from humans in Japan. Appl Environ Microbiol. 1991 Aug;57(8):2152–2157. [PMC free article] [PubMed]
  • Miyamoto Y, Kato T, Obara Y, Akiyama S, Takizawa K, Yamai S. In vitro hemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J Bacteriol. 1969 Nov;100(2):1147–1149. [PMC free article] [PubMed]
  • Mukhopadhyay AK, Garg S, Mitra R, Basu A, Rajendran K, Dutta D, Bhattacharya SK, Shimada T, Takeda T, Takeda Y, et al. Temporal shifts in traits of Vibrio cholerae strains isolated from hospitalized patients in Calcutta: a 3-year (1993 to 1995) analysis. J Clin Microbiol. 1996 Oct;34(10):2537–2543. [PMC free article] [PubMed]
  • Nishibuchi M, Kaper JB. Duplication and variation of the thermostable direct haemolysin (tdh) gene in Vibrio parahaemolyticus. Mol Microbiol. 1990 Jan;4(1):87–99. [PubMed]
  • Nishibuchi M, Kumagai K, Kaper JB. Contribution of the tdh1 gene of Kanagawa phenomenon-positive Vibrio parahaemolyticus to production of extracellular thermostable direct hemolysin. Microb Pathog. 1991 Dec;11(6):453–460. [PubMed]
  • Nishibuchi M, Taniguchi T, Misawa T, Khaeomanee-Iam V, Honda T, Miwatani T. Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus. Infect Immun. 1989 Sep;57(9):2691–2697. [PMC free article] [PubMed]
  • Nolan CM, Ballard J, Kaysner CA, Lilja JL, Williams LP, Jr, Tenover FC. Vibrio parahaemolyticus gastroenteritis. An outbreak associated with raw oysters in the Pacific northwest. Diagn Microbiol Infect Dis. 1984 Apr;2(2):119–128. [PubMed]
  • Obata H, Kai A, Sekiguchi K, Matsushita S, Yamada S, Itoh T, Ohta K, Kudoh Y. [Detection of the trh gene in Vibrio parahaemolyticus isolated from overseas travellers' diarrhea and their biochemical characteristics]. Kansenshogaku Zasshi. 1996 Aug;70(8):815–820. [PubMed]
  • Okuda J, Ishibashi M, Abbott SL, Janda JM, Nishibuchi M. Analysis of the thermostable direct hemolysin (tdh) gene and the tdh-related hemolysin (trh) genes in urease-positive strains of Vibrio parahaemolyticus isolated on the West Coast of the United States. J Clin Microbiol. 1997 Aug;35(8):1965–1971. [PMC free article] [PubMed]
  • Osawa R, Okitsu T, Morozumi H, Yamai S. Occurrence of urease-positive Vibrio parahaemolyticus in Kanagawa, Japan, with specific reference to presence of thermostable direct hemolysin (TDH) and the TDH-related-hemolysin genes. Appl Environ Microbiol. 1996 Feb;62(2):725–727. [PMC free article] [PubMed]
  • Sakazaki R, Tamura K, Kato T, Obara Y, Yamai S. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. 3. Enteropathogenicity. Jpn J Med Sci Biol. 1968 Oct;21(5):325–331. [PubMed]
  • Shirai H, Ito H, Hirayama T, Nakamoto Y, Nakabayashi N, Kumagai K, Takeda Y, Nishibuchi M. Molecular epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis. Infect Immun. 1990 Nov;58(11):3568–3573. [PMC free article] [PubMed]
  • Suthienkul O, Ishibashi M, Iida T, Nettip N, Supavej S, Eampokalap B, Makino M, Honda T. Urease production correlates with possession of the trh gene in Vibrio parahaemolyticus strains isolated in Thailand. J Infect Dis. 1995 Nov;172(5):1405–1408. [PubMed]
  • Suzuki N, Ueda Y, Mori H, Miyagi K, Noda K, Hirose H, Oosumi Y, Ishibashi M, Yoh M, Yamamoto K, et al. [Serotypes of urease producing Vibrio parahaemolyticus and their relation to possession of tdh and trh genes]. Kansenshogaku Zasshi. 1994 Sep;68(9):1068–1074. [PubMed]
  • Suzuki N, Ueda Y, Mori H, Takegaki Y, Miyagi K, Noda K, Hirose K, Hashimoto S, Oosumi Y, Ishibashi M, et al. [Correlation between trh possession and urease production of clinical isolates of Vibrio parahaemolyticus]. Kansenshogaku Zasshi. 1995 Jun;69(6):757–758. [PubMed]
  • Takeda Y. Thermostable direct hemolysin of Vibrio parahaemolyticus. Pharmacol Ther. 1982;19(1):123–146. [PubMed]
  • Terai A, Baba K, Shirai H, Yoshida O, Takeda Y, Nishibuchi M. Evidence for insertion sequence-mediated spread of the thermostable direct hemolysin gene among Vibrio species. J Bacteriol. 1991 Aug;173(16):5036–5046. [PMC free article] [PubMed]
  • Waldor MK, Mekalanos JJ. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science. 1996 Jun 28;272(5270):1910–1914. [PubMed]

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