• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Nov 1997; 35(11): 2841–2845.
PMCID: PMC230072

Antigenic lipopolysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division of the species into serogroups.

Abstract

Legionella pneumophila accounts for the majority of cases of Legionnaires' disease. By using rabbit antisera, the species has been divided into 14 numbered and 1 unnumbered serogroups. To recognize the antigenic diversity of the lipopolysaccharide (LPS) responsible for this classification, the Dresden Legionella LPS MAb panel, containing 98 monoclonal antibodies (MAbs), was created. Each serogroup reference strain possesses at least one specific epitope not found on any other reference strain and therefore designated the serogroup-specific epitope. When the appropriate MAbs were used for serotyping of 1,064 human and environmental isolates, 1,045 (98%) could be placed into the known serogroups. In most cases (97%), this was in agreement with the polyclonal typing. Of the 29 isolates that showed strong cross-reactivities with the rabbit antiserum panel, 11 could be typed easily by MAbs; for the remaining 18, however, only serogroup-cross-reactive epitopes could be determined. Below the serogroup level, monoclonal subtypes were found for 11 serogroups. Altogether, the Dresden Legionella LPS MAb panel was able to divide the 1,064 isolates tested into 64 phenons, indicating its usefulness for both serogrouping and subgrouping of L. pneumophila strains. In order to compare the identities of patient and environmental isolates, testing their reactivity with MAbs should be the first step, especially if large numbers of colonies are to be typed. Only in cases of identical patterns are the more time consuming and expensive genetic fingerprints necessary. Moreover, the MAbs can also be used for specific antigen detection in respiratory specimens on the serogroup or subgroup level.

Full Text

The Full Text of this article is available as a PDF (136K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Benson RF, Thacker WL, Daneshvar MI, Brenner DJ. Legionella waltersii sp. nov. and an unnamed Legionella genomospecies isolated from water in Australia. Int J Syst Bacteriol. 1996 Jul;46(3):631–634. [PubMed]
  • Brenner DJ, Steigerwalt AG, Epple P, Bibb WF, McKinney RM, Starnes RW, Colville JM, Selander RK, Edelstein PH, Moss CW. Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov. J Clin Microbiol. 1988 Sep;26(9):1695–1703. [PMC free article] [PubMed]
  • Brindle RJ, Bryant TN, Draper PW. Taxonomic investigation of Legionella pneumophila using monoclonal antibodies. J Clin Microbiol. 1989 Mar;27(3):536–539. [PMC free article] [PubMed]
  • Cianfriglia M, Armellini D, Massone A, Mariani M. Simple immunization protocol for high frequency production of soluble antigen-specific hybridomas. Hybridoma. 1983;2(4):451–457. [PubMed]
  • Ciesielski CA, Blaser MJ, Wang WL. Serogroup specificity of Legionella pneumophila is related to lipopolysaccharide characteristics. Infect Immun. 1986 Feb;51(2):397–404. [PMC free article] [PubMed]
  • Joly JR, McKinney RM, Tobin JO, Bibb WF, Watkins ID, Ramsay D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol. 1986 Apr;23(4):768–771. [PMC free article] [PubMed]
  • McDade JE, Shepard CC, Fraser DW, Tsai TR, Redus MA, Dowdle WR. Legionnaires' disease: isolation of a bacterium and demonstration of its role in other respiratory disease. N Engl J Med. 1977 Dec 1;297(22):1197–1203. [PubMed]
  • Peters H, Jürs M, Jann B, Jann K, Timmis KN, Bitter-Suermann D. Monoclonal antibodies to enterobacterial common antigen and to Escherichia coli lipopolysaccharide outer core: demonstration of an antigenic determinant shared by enterobacterial common antigen and E. coli K5 capsular polysaccharide. Infect Immun. 1985 Nov;50(2):459–466. [PMC free article] [PubMed]
  • Reingold AL, Thomason BM, Brake BJ, Thacker L, Wilkinson HW, Kuritsky JN. Legionella pneumonia in the United States: the distribution of serogroups and species causing human illness. J Infect Dis. 1984 May;149(5):819–819. [PubMed]
  • Van Belkum A, Maas H, Verbrugh H, Van Leeuwen N. Serotyping, ribotyping, PCR-mediated ribosomal 16S-23S spacer analysis and arbitrarily primed PCR for epidemiological studies on Legionella pneumophila. Res Microbiol. 1996 Jun;147(5):405–413. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links