• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Jun 1997; 35(6): 1633–1635.
PMCID: PMC229809

Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR.

Abstract

In a previous study using pure bacterial cultures in a PCR assay, a primer pair corresponding to a unique chromosomal region of Vibrio cholerae O139 Bengal generated an amplicon from only V. cholerae O139 Bengal. PCR with the same primer pair was used to screen 180 diarrheal stool specimens. All the 67 V. cholerae O139 culture-positive stool specimens were positive by PCR, and the remaining specimens, which contained either other recognized enteric pathogens or no pathogens, were all negative by PCR.

Full Text

The Full Text of this article is available as a PDF (128K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Albert MJ. Vibrio cholerae O139 Bengal. J Clin Microbiol. 1994 Oct;32(10):2345–2349. [PMC free article] [PubMed]
  • Albert MJ, Faruque SM, Faruque AS, Neogi PK, Ansaruzzaman M, Bhuiyan NA, Alam K, Akbar MS. Controlled study of Escherichia coli diarrheal infections in Bangladeshi children. J Clin Microbiol. 1995 Apr;33(4):973–977. [PMC free article] [PubMed]
  • Ansaruzzaman M, Rahman M, Kibriya AK, Bhuiyan NA, Islam MS, Albert MJ. Isolation of sucrose late-fermenting and nonfermenting variants of Vibrio cholerae O139 Bengal: implications for diagnosis of cholera. J Clin Microbiol. 1995 May;33(5):1339–1340. [PMC free article] [PubMed]
  • Bik EM, Bunschoten AE, Gouw RD, Mooi FR. Genesis of the novel epidemic Vibrio cholerae O139 strain: evidence for horizontal transfer of genes involved in polysaccharide synthesis. EMBO J. 1995 Jan 16;14(2):209–216. [PMC free article] [PubMed]
  • Cheasty T, Rowe B, Said B, Frost J. Vibrio cholerae serogroup 0139 in England and Wales. BMJ. 1993 Oct 16;307(6910):1007–1007. [PMC free article] [PubMed]
  • Chongsa-nguan M, Chaicumpa W, Moolasart P, Kandhasingha P, Shimada T, Kurazono H, Takeda Y. Vibrio cholerae O139 Bengal in Bangkok. Lancet. 1993 Aug 14;342(8868):430–431. [PubMed]
  • Comstock LE, Johnson JA, Michalski JM, Morris JG, Jr, Kaper JB. Cloning and sequence of a region encoding a surface polysaccharide of Vibrio cholerae O139 and characterization of the insertion site in the chromosome of Vibrio cholerae O1. Mol Microbiol. 1996 Feb;19(4):815–826. [PubMed]
  • Falklind S, Stark M, Albert MJ, Uhlén M, Lundeberg J, Weintraub A. Cloning and sequence of a region of Vibrio cholerae O139 Bengal and its use in PCR-based detection. J Clin Microbiol. 1996 Dec;34(12):2904–2908. [PMC free article] [PubMed]
  • Fisher-Hoch SP, Khan A, Inam-ul-Haq, Khan MA, Mintz ED. Vibrio cholerae O139 in Karachi, Pakistan. Lancet. 1993 Dec 4;342(8884):1422–1423. [PubMed]
  • Knirel YA, Paredes L, Jansson PE, Weintraub A, Widmalm G, Albert MJ. Structure of the capsular polysaccharide of Vibrio cholerae O139 synonym Bengal containing D-galactose 4,6-cyclophosphate. Eur J Biochem. 1995 Sep 1;232(2):391–396. [PubMed]
  • Nair GB, Bag PK, Shimada T, Ramamurthy T, Takeda T, Yamamoto S, Kurazono H, Takeda Y. Evaluation of DNA probes for specific detection of Vibrio cholerae O139 Bengal. J Clin Microbiol. 1995 Aug;33(8):2186–2187. [PMC free article] [PubMed]
  • Popovic T, Fields PI, Olsvik O, Wells JG, Evins GM, Cameron DN, Farmer JJ, 3rd, Bopp CA, Wachsmuth K, Sack RB, et al. Molecular subtyping of toxigenic Vibrio cholerae O139 causing epidemic cholera in India and Bangladesh, 1992-1993. J Infect Dis. 1995 Jan;171(1):122–127. [PubMed]
  • Qadri F, Hasan JA, Hossain J, Chowdhury A, Begum YA, Azim T, Loomis L, Sack RB, Albert MJ. Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples. J Clin Microbiol. 1995 Mar;33(3):732–734. [PMC free article] [PubMed]
  • Stroeher UH, Jedani KE, Dredge BK, Morona R, Brown MH, Karageorgos LE, Albert MJ, Manning PA. Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139. Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10374–10378. [PMC free article] [PubMed]
  • Wyk P, Reeves P. Identification and sequence of the gene for abequose synthase, which confers antigenic specificity on group B salmonellae: homology with galactose epimerase. J Bacteriol. 1989 Oct;171(10):5687–5693. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...