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J Clin Microbiol. Jun 1997; 35(6): 1530–1532.
PMCID: PMC229780

Evaluation of a rapid enzyme immunoassay for diagnosis of hepatic amoebiasis.


We compared the capability of rapid enzyme immunoassay (EIA) to detect antiamoebic antibodies during hepatic amoebiasis with those of indirect hemagglutination and latex agglutination. EIA is simple to perform and rapid (20 min) and does not require any special equipment (optical reading is sufficient). EIA of 143 sera (including 43 from patients with proven hepatic amoebic abscess, 33 from patients with other hepatic disorders and/or parasitic infections, and 67 from healthy individuals) yielded a specificity, a sensitivity, and positive and negative predictive values of 100, 93, 100, and 97.1, respectively. This test could thus be considered another valuable tool for the diagnosis of hepatic amoebiasis.

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Selected References

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  • Boisseau H, Appriou M, Lucchese F, Cabannes A, Tribouley-Duret J, Tribouley J. Evaluation d'un test d'hémagglutination indirecte amibiase (HAI) pour le serodiagnostic de l'amibiase. Med Trop (Mars) 1992 Jul-Sep;52(3):307–311. [PubMed]
  • Gandhi BM, Irshad M, Chawla TC, Tandon BN. Enzyme linked protein-A: an ELISA for detection of amoebic antibody. Trans R Soc Trop Med Hyg. 1987;81(2):183–185. [PubMed]
  • Hartmann DP, Ghadirian E, Meerovitch E. Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination (IHA) test in the serodiagnosis of experimental hepatic amebiasis. J Parasitol. 1980 Apr;66(2):344–345. [PubMed]
  • Jacquier P, Gottstein B, Stingelin Y, Eckert J. Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. J Clin Microbiol. 1991 Sep;29(9):1831–1835. [PMC free article] [PubMed]
  • Kozinn PJ, Taschdjian CL, Goldberg PK, Protzmann WP, MacKenzie DW, Remington JS, Anderson S, Seelig MS. Efficiency of serologic tests in the diagnosis of systemic candidiasis. Am J Clin Pathol. 1978 Dec;70(6):893–898. [PubMed]
  • Maddison SE. Serodiagnosis of parasitic diseases. Clin Microbiol Rev. 1991 Oct;4(4):457–469. [PMC free article] [PubMed]
  • Ravdin JI. Amebiasis. Clin Infect Dis. 1995 Jun;20(6):1453–1466. [PubMed]
  • Robert R, Mahaza C, Bernard C, Buffard C, Senet JM. Evaluation of a new bicolored latex agglutination test for immunological diagnosis of hepatic amoebiasis. J Clin Microbiol. 1990 Jun;28(6):1422–1424. [PMC free article] [PubMed]
  • Sathar MA, Simjee AE, Nel JD, Bredenkamp BL, Gathiram V, Jackson TF. Evaluation of an enzyme-linked immunosorbent assay in the serodiagnosis of amoebic liver abscess. S Afr Med J. 1988 Dec 17;74(12):625–628. [PubMed]
  • Sloan L, Schneider S, Rosenblatt J. Evaluation of enzyme-linked immunoassay for serological diagnosis of cysticercosis. J Clin Microbiol. 1995 Dec;33(12):3124–3128. [PMC free article] [PubMed]
  • Pahuchonma W, Janitschke K. Serodiagnosis of parasitic infections by ELISA with different antigens. Southeast Asian J Trop Med Public Health. 1987 Jun;18(2):193–196. [PubMed]
  • Yang J, Kennedy MT. Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis. J Clin Microbiol. 1979 Dec;10(6):778–785. [PMC free article] [PubMed]

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