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J Clin Microbiol. Jun 1997; 35(6): 1304–1310.
PMCID: PMC229739

PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems.


Human papillomavirus (HPV) is an etiologic agent of cervical cancer and is the most common sexually transmitted disease in women. PCR amplification of HPV genomes is the most sensitive method for the detection of cervicovaginal HPV. We have compared the two most commonly used PCR primer sets, MY09/MY11 (MY-PCR) and GP5+/GP6+ (GP+-PCR), for the detection of HPV DNA in cervicovaginal lavage samples from 208 women. Oligonucleotide probes for 39 different HPV types were used. Both primer sets amplified a wide spectrum of HPV genotypes and detected similar overall prevalences of 45% (94 of 208) and 43% (89 of 208), respectively. The MY-PCR system detected 27 of 30 (90%) samples with multiple HPV types, whereas the GP+-PCR system detected 14 of 30 (47%) samples with multiple HPV types. Differences in the detection of HPV types 35, 53, and 61 were noted between the two primer systems. Serial dilution of plasmid templates indicated a 3-log decrease in the amplification of HPV type 35 by MY-PCR and HPV types 53 and 61 by GP+-PCR. These results indicate that although the MY-PCR and GP+-PCR identified nearly equivalent prevalences of HPV in a set of clinical samples, differences in the detection of specific types and infections with multiple types were found. Differences in the sensitivities and characteristics of the PCR systems for the detection of HPV within clinical samples should be considered when comparing data between studies and/or in designing new studies or clinical trials.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Baay MF, Quint WG, Koudstaal J, Hollema H, Duk JM, Burger MP, Stolz E, Herbrink P. Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas. J Clin Microbiol. 1996 Mar;34(3):745–747. [PMC free article] [PubMed]
  • Bauer HM, Hildesheim A, Schiffman MH, Glass AG, Rush BB, Scott DR, Cadell DM, Kurman RJ, Manos MM. Determinants of genital human papillomavirus infection in low-risk women in Portland, Oregon. Sex Transm Dis. 1993 Sep-Oct;20(5):274–278. [PubMed]
  • Bauer HM, Ting Y, Greer CE, Chambers JC, Tashiro CJ, Chimera J, Reingold A, Manos MM. Genital human papillomavirus infection in female university students as determined by a PCR-based method. JAMA. 1991 Jan 23;265(4):472–477. [PubMed]
  • Bosch FX, Manos MM, Muñoz N, Sherman M, Jansen AM, Peto J, Schiffman MH, Moreno V, Kurman R, Shah KV. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group. J Natl Cancer Inst. 1995 Jun 7;87(11):796–802. [PubMed]
  • Burk RD, Ho GY, Beardsley L, Lempa M, Peters M, Bierman R. Sexual behavior and partner characteristics are the predominant risk factors for genital human papillomavirus infection in young women. J Infect Dis. 1996 Oct;174(4):679–689. [PubMed]
  • Delius H, Hofmann B. Primer-directed sequencing of human papillomavirus types. Curr Top Microbiol Immunol. 1994;186:13–31. [PubMed]
  • de Roda Husman AM, Walboomers JM, van den Brule AJ, Meijer CJ, Snijders PJ. The use of general primers GP5 and GP6 elongated at their 3' ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol. 1995 Apr;76(Pt 4):1057–1062. [PubMed]
  • de Roda Husman AM, Walboomers JM, Hopman E, Bleker OP, Helmerhorst TM, Rozendaal L, Voorhorst FJ, Meijer CJ. HPV prevalence in cytomorphologically normal cervical scrapes of pregnant women as determined by PCR: the age-related pattern. J Med Virol. 1995 Jun;46(2):97–102. [PubMed]
  • de Roda Husman AM, Walboomers JM, Meijer CJ, Risse EK, Schipper ME, Helmerhorst TM, Bleker OP, Delius H, van den Brule AJ, Snijders PJ. Analysis of cytomorphologically abnormal cervical scrapes for the presence of 27 mucosotropic human papillomavirus genotypes, using polymerase chain reaction. Int J Cancer. 1994 Mar 15;56(6):802–806. [PubMed]
  • Eluf-Neto J, Booth M, Muñoz N, Bosch FX, Meijer CJ, Walboomers JM. Human papillomavirus and invasive cervical cancer in Brazil. Br J Cancer. 1994 Jan;69(1):114–119. [PMC free article] [PubMed]
  • Franco EL. Measurement errors in epidemiological studies of human papillomavirus and cervical cancer. IARC Sci Publ. 1992;(119):181–197. [PubMed]
  • Gallahan D, Müller M, Schneider A, Delius H, Kahn T, de Villiers EM, Gissmann L. Human papillomavirus type 53. J Virol. 1989 Nov;63(11):4911–4912. [PMC free article] [PubMed]
  • Guerrero E, Daniel RW, Bosch FX, Castellsagué X, Muñoz N, Gili M, Viladiu P, Navarro C, Zubiri ML, Ascunce N, et al. Comparison of ViraPap, Southern hybridization, and polymerase chain reaction methods for human papillomavirus identification in an epidemiological investigation of cervical cancer. J Clin Microbiol. 1992 Nov;30(11):2951–2959. [PMC free article] [PubMed]
  • al-Bekairi AM, Qureshi S, Chaudhry MA, Shah AH. Uric acid as an inhibitor of cyclophosphamide-induced micronuclei in mice. Mutat Res. 1991 Feb;262(2):115–118. [PubMed]
  • Hildesheim A, Gravitt P, Schiffman MH, Kurman RJ, Barnes W, Jones S, Tchabo JG, Brinton LA, Copeland C, Epp J, et al. Determinants of genital human papillomavirus infection in low-income women in Washington, D.C. Sex Transm Dis. 1993 Sep-Oct;20(5):279–285. [PubMed]
  • Hildesheim A, Schiffman MH, Gravitt PE, Glass AG, Greer CE, Zhang T, Scott DR, Rush BB, Lawler P, Sherman ME, et al. Persistence of type-specific human papillomavirus infection among cytologically normal women. J Infect Dis. 1994 Feb;169(2):235–240. [PubMed]
  • Ho GY, Burk RD, Fleming I, Klein RS. Risk of genital human papillomavirus infection in women with human immunodeficiency virus-induced immunosuppression. Int J Cancer. 1994 Mar 15;56(6):788–792. [PubMed]
  • Ho GY, Burk RD, Klein S, Kadish AS, Chang CJ, Palan P, Basu J, Tachezy R, Lewis R, Romney S. Persistent genital human papillomavirus infection as a risk factor for persistent cervical dysplasia. J Natl Cancer Inst. 1995 Sep 20;87(18):1365–1371. [PubMed]
  • Hsing AW, Burk RD, Liaw KL, Chen CJ, Zhang T, Schiffman M, Greer CE, You SL, Hsieh CY, Huang TW, et al. Interlaboratory agreement in a polymerase chain reaction-based human papillomavirus DNA assay. Cancer Epidemiol Biomarkers Prev. 1996 Jun;5(6):483–484. [PubMed]
  • Jacobs MV, de Roda Husman AM, van den Brule AJ, Snijders PJ, Meijer CJ, Walboomers JM. Group-specific differentiation between high- and low-risk human papillomavirus genotypes by general primer-mediated PCR and two cocktails of oligonucleotide probes. J Clin Microbiol. 1995 Apr;33(4):901–905. [PMC free article] [PubMed]
  • Jiang G, Qu W, Ruan H, Burk RD. Elimination of false-positive signals in enhanced chemiluminescence (ECL) detection of amplified HPV DNA from clinical samples. Biotechniques. 1995 Oct;19(4):566–568. [PubMed]
  • Karlsen F, Kalantari M, Jenkins A, Pettersen E, Kristensen G, Holm R, Johansson B, Hagmar B. Use of multiple PCR primer sets for optimal detection of human papillomavirus. J Clin Microbiol. 1996 Sep;34(9):2095–2100. [PMC free article] [PubMed]
  • Klein RS, Ho GY, Vermund SH, Fleming I, Burk RD. Risk factors for squamous intraepithelial lesions on Pap smear in women at risk for human immunodeficiency virus infection. J Infect Dis. 1994 Dec;170(6):1404–1409. [PubMed]
  • Kuypers JM, Critchlow CW, Gravitt PE, Vernon DA, Sayer JB, Manos MM, Kiviat NB. Comparison of dot filter hybridization, Southern transfer hybridization, and polymerase chain reaction amplification for diagnosis of anal human papillomavirus infection. J Clin Microbiol. 1993 Apr;31(4):1003–1006. [PMC free article] [PubMed]
  • Ley C, Bauer HM, Reingold A, Schiffman MH, Chambers JC, Tashiro CJ, Manos MM. Determinants of genital human papillomavirus infection in young women. J Natl Cancer Inst. 1991 Jul 17;83(14):997–1003. [PubMed]
  • Liaw KL, Hsing AW, Chen CJ, Schiffman MH, Zhang TY, Hsieh CY, Greer CE, You SL, Huang TW, Wu TC, et al. Human papillomavirus and cervical neoplasia: a case-control study in Taiwan. Int J Cancer. 1995 Sep 4;62(5):565–571. [PubMed]
  • Melkert PW, Hopman E, van den Brule AJ, Risse EK, van Diest PJ, Bleker OP, Helmerhorst T, Schipper ME, Meijer CJ, Walboomers JM. Prevalence of HPV in cytomorphologically normal cervical smears, as determined by the polymerase chain reaction, is age-dependent. Int J Cancer. 1993 Apr 1;53(6):919–923. [PubMed]
  • Morris BJ, Rose BR, Flanagan JL, McKinnon KJ, Loo CY, Thompson CH, Flampoulidou M, Ford RM, Hunter JC, Nightingale BN, et al. Automated polymerase chain reaction for papillomavirus screening of cervicovaginal lavages: comparison with dot-blot hybridization in a sexually transmitted diseases clinic population. J Med Virol. 1990 Sep;32(1):22–30. [PubMed]
  • Morrison EA, Ho GY, Vermund SH, Goldberg GL, Kadish AS, Kelley KF, Burk RD. Human papillomavirus infection and other risk factors for cervical neoplasia: a case-control study. Int J Cancer. 1991 Aug 19;49(1):6–13. [PubMed]
  • Remmink AJ, Walboomers JM, Helmerhorst TJ, Voorhorst FJ, Rozendaal L, Risse EK, Meijer CJ, Kenemans P. The presence of persistent high-risk HPV genotypes in dysplastic cervical lesions is associated with progressive disease: natural history up to 36 months. Int J Cancer. 1995 May 4;61(3):306–311. [PubMed]
  • Schiffman MH, Bauer HM, Hoover RN, Glass AG, Cadell DM, Rush BB, Scott DR, Sherman ME, Kurman RJ, Wacholder S, et al. Epidemiologic evidence showing that human papillomavirus infection causes most cervical intraepithelial neoplasia. J Natl Cancer Inst. 1993 Jun 16;85(12):958–964. [PubMed]
  • Schiffman MH, Bauer HM, Lorincz AT, Manos MM, Byrne JC, Glass AG, Cadell DM, Howley PM. Comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA. J Clin Microbiol. 1991 Mar;29(3):573–577. [PMC free article] [PubMed]
  • Schiffman MH, Schatzkin A. Test reliability is critically important to molecular epidemiology: an example from studies of human papillomavirus infection and cervical neoplasia. Cancer Res. 1994 Apr 1;54(7 Suppl):1944s–1947s. [PubMed]
  • Smits HL, Bollen LJ, Tjong-A-Hung SP, Vonk J, Van Der Velden J, Ten Kate FJ, Kaan JA, Mol BW, Ter Schegget J. Intermethod variation in detection of human papillomavirus DNA in cervical smears. J Clin Microbiol. 1995 Oct;33(10):2631–2636. [PMC free article] [PubMed]
  • Snijders PJ, van den Brule AJ, Schrijnemakers HF, Snow G, Meijer CJ, Walboomers JM. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. J Gen Virol. 1990 Jan;71(Pt 1):173–181. [PubMed]
  • Stewart AC, Eriksson AM, Manos MM, Muñoz N, Bosch FX, Peto J, Wheeler CM. Intratype variation in 12 human papillomavirus types: a worldwide perspective. J Virol. 1996 May;70(5):3127–3136. [PMC free article] [PubMed]
  • Tachezy R, Van Ranst MA, Cruz Y, Burk RD. Analysis of short novel human papillomavirus sequences. Biochem Biophys Res Commun. 1994 Oct 28;204(2):820–827. [PubMed]
  • Tucker RA, Johnson PR, Reeves WC, Icenogle JP. Using the polymerase chain reaction to genotype human papillomavirus DNAs in samples containing multiple HPVs may produce inaccurate results. J Virol Methods. 1993 Aug;43(3):321–333. [PubMed]
  • Wan CY, Wilkins TA. Spermidine facilitates PCR amplification of target DNA. PCR Methods Appl. 1993 Dec;3(3):208–210. [PubMed]
  • Ward P, Parry GN, Yule R, Coleman DV, Malcolm AD. Comparison between the polymerase chain reaction and slot blot hybridization for the detection of HPV sequences in cervical scrapes. Cytopathology. 1990;1(1):19–23. [PubMed]
  • Wheeler CM, Parmenter CA, Hunt WC, Becker TM, Greer CE, Hildesheim A, Manos MM. Determinants of genital human papillomavirus infection among cytologically normal women attending the University of New Mexico student health center. Sex Transm Dis. 1993 Sep-Oct;20(5):286–289. [PubMed]

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