• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Apr 1997; 35(4): 995–998.
PMCID: PMC229720

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.


A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.

Full Text

The Full Text of this article is available as a PDF (459K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brian MJ, Frosolono M, Murray BE, Miranda A, Lopez EL, Gomez HF, Cleary TG. Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome. J Clin Microbiol. 1992 Jul;30(7):1801–1806. [PMC free article] [PubMed]
  • Demeke T, Adams RP. The effects of plant polysaccharides and buffer additives on PCR. Biotechniques. 1992 Mar;12(3):332–334. [PubMed]
  • Do N, Adams RP. A simple technique for removing plant polysaccharide contaminants from DNA. Biotechniques. 1991 Feb;10(2):162–166. [PubMed]
  • Fang G, Hammar S, Grumet R. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques. 1992 Jul;13(1):52–56. [PubMed]
  • Frankel G, Riley L, Giron JA, Valmassoi J, Friedmann A, Strockbine N, Falkow S, Schoolnik GK. Detection of Shigella in feces using DNA amplification. J Infect Dis. 1990 Jun;161(6):1252–1256. [PubMed]
  • Furukawa K, Bhavanandan VP. Influences of anionic polysaccharides on DNA synthesis in isolated nuclei and by DNA polymerase alpha: correlation of observed effects with properties of the polysaccharides. Biochim Biophys Acta. 1983 Sep 9;740(4):466–475. [PubMed]
  • Hammar M, Tyszkiewicz T, Wadström T, O'Toole PW. Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction. J Clin Microbiol. 1992 Jan;30(1):54–58. [PMC free article] [PubMed]
  • Ho SA, Hoyle JA, Lewis FA, Secker AD, Cross D, Mapstone NP, Dixon MF, Wyatt JI, Tompkins DS, Taylor GR, et al. Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals. J Clin Microbiol. 1991 Nov;29(11):2543–2549. [PMC free article] [PubMed]
  • Islam D, Lindberg AA. Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction. J Clin Microbiol. 1992 Nov;30(11):2801–2806. [PMC free article] [PubMed]
  • Jiang X, Wang J, Graham DY, Estes MK. Detection of Norwalk virus in stool by polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2529–2534. [PMC free article] [PubMed]
  • de Lamballerie X, Zandotti C, Vignoli C, Bollet C, de Micco P. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res Microbiol. 1992 Oct;143(8):785–790. [PubMed]
  • Liu C, Smith BM, Ajito K, Komatsu H, Gomez-Paloma L, Li T, Theodorakis EA, Nicolaou KC, Vogt PK. Sequence-selective carbohydrate-DNA interaction: dimeric and monomeric forms of the calicheamicin oligosaccharide interfere with transcription factor function. Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):940–944. [PMC free article] [PubMed]
  • Lund A, Wasteson Y, Olsvik O. Immunomagnetic separation and DNA hybridization for detection of enterotoxigenic Escherichia coli in a piglet model. J Clin Microbiol. 1991 Oct;29(10):2259–2262. [PMC free article] [PubMed]
  • Mapstone NP, Lynch DA, Lewis FA, Axon AT, Tompkins DS, Dixon MF, Quirke P. PCR identification of Helicobacter pylori in faeces from gastritis patients. Lancet. 1993 Feb 13;341(8842):447–447. [PubMed]
  • Shirai H, Nishibuchi M, Ramamurthy T, Bhattacharya SK, Pal SC, Takeda Y. Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae. J Clin Microbiol. 1991 Nov;29(11):2517–2521. [PMC free article] [PubMed]
  • Siebert PD, Larrick JW. PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR. Biotechniques. 1993 Feb;14(2):244–249. [PubMed]
  • van Zwet AA, Thijs JC, Kooistra-Smid AM, Schirm J, Snijder JA. Use of PCR with feces for detection of Helicobacter pylori infections in patients. J Clin Microbiol. 1994 May;32(5):1346–1348. [PMC free article] [PubMed]
  • Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 1991 Apr;10(4):506–513. [PubMed]
  • Wilde J, Eiden J, Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol. 1990 Jun;28(6):1300–1307. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...