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J Clin Microbiol. Dec 1996; 34(12): 2881–2887.
PMCID: PMC229427

Influence of relative humidity and suspending menstrua on survival of Acinetobacter spp. on dry surfaces.

Abstract

Acinetobacter spp. are being reported with increasing frequency as a cause of nosocomial infection and have been isolated from the skin of healthy individuals, patients, hospital staff, dry nonbiotic objects, and different pieces of medical equipment. Factors affecting the survival of Acinetobacter spp. under conditions closely similar to those found in the hospital environment were investigated in the present study to help us understand the epidemiology of nosocomial Acinetobacter infection. Bacterial cells were suspended in distilled water or bovine serum albumin and were dried onto glass coverslips and kept at different relative humidities. Cells washed from coverslips were used to determined viable counts. Freshly isolated strains of Acinetobacter spp. belonging to the clinically important Acinetobacter calcoaceticus-Acinetobacter baumannii complex were found to be more resistant to drying conditions (e.g., 30 days for A. baumannii 16/49) than American Type Culture Collection strains (e.g., 2 days for A. baumannii ATCC 9955). The majority of strains belonging to the Acb complex had survival times similar to those observed for the gram-positive organism Staphylococcus aureus tested in the experiment. Survival times were prolonged for almost all the strains tested when they were suspended in bovine serum albumin (e.g., 60 days for A. baumannii R 447) compared with those for strains suspended in distilled water (11 days for R 447). The survival times for strains at higher relative humidity (31 or 93%) were longer than those for strains of Acinetobacter kept at a relative humidity of 10% (11 days at 31% relative humidity and 4 days at 10% relative humidity for R447). These findings are consistent with the observed tendency of Acinetobacter spp. to survive on dry surfaces, and they can be transferred not only by moist vectors but also under dry conditions in a hospital environment during nosocomial infection outbreaks. The results obtained in the experiment support the previously suggested airborne spread of Acinetobacter spp. in hospital wards and repeated outbreaks after incomplete disinfection of contaminated dry surfaces.

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Selected References

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  • Allen KD, Green HT. Hospital outbreak of multi-resistant Acinetobacter anitratus: an airborne mode of spread? J Hosp Infect. 1987 Mar;9(2):110–119. [PubMed]
  • Anstey NM, Currie BJ, Withnall KM. Community-acquired Acinetobacter pneumonia in the Northern Territory of Australia. Clin Infect Dis. 1992 Jan;14(1):83–91. [PubMed]
  • Asada S, Takano M, Shibasaki I. Deoxyribonucleic acid strand breaks during drying of Escherichia coli on a hydorohobic filter membrane. Appl Environ Microbiol. 1979 Feb;37(2):266–273. [PMC free article] [PubMed]
  • Baumann P. Isolation of Acinetobacter from soil and water. J Bacteriol. 1968 Jul;96(1):39–42. [PMC free article] [PubMed]
  • Bergogne-Bérézin E, Towner KJ. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev. 1996 Apr;9(2):148–165. [PMC free article] [PubMed]
  • Christie C, Mazon D, Hierholzer W, Jr, Patterson JE. Molecular heterogeneity of Acinetobacter baumanii isolates during seasonal increase in prevalence. Infect Control Hosp Epidemiol. 1995 Oct;16(10):590–594. [PubMed]
  • Crowe M, Towner KJ, Humphreys H. Clinical and epidemiological features of an outbreak of acinetobacter infection in an intensive therapy unit. J Med Microbiol. 1995 Jul;43(1):55–62. [PubMed]
  • Cunha BA, Klimek JJ, Gracewski J, McLaughlin JC, Quintiliani R. A common source outbreak of Acinetobacter pulmonary infections traced to Wright respirometers. Postgrad Med J. 1980 Mar;56(653):169–172. [PMC free article] [PubMed]
  • Dickgiesser N, Ludwig C. Untersuchungen über das Verhalten grampositiver und gramnegativer Bakterien auf Aluminiumfolie. Zentralbl Bakteriol B. 1979 Jun;168(5-6):493–506. [PubMed]
  • French GL, Casewell MW, Roncoroni AJ, Knight S, Phillips I. A hospital outbreak of antibiotic-resistant Acinetobacter anitratus: epidemiology and control. J Hosp Infect. 1980 Jun;1(2):125–131. [PubMed]
  • Gerner-Smidt P. Endemic occurrence of Acinetobacter calcoaceticus biovar anitratus in an intensive care unit. J Hosp Infect. 1987 Nov;10(3):265–272. [PubMed]
  • Gerner-Smidt P, Tjernberg I, Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol. 1991 Feb;29(2):277–282. [PMC free article] [PubMed]
  • Getchell-White SI, Donowitz LG, Gröschel DH. The inanimate environment of an intensive care unit as a potential source of nosocomial bacteria: evidence for long survival of Acinetobacter calcoaceticus. Infect Control Hosp Epidemiol. 1989 Sep;10(9):402–407. [PubMed]
  • Heller G. A Quantitative Study of Environmental Factors Involved in Survival and Death of Bacteria in the Desiccated State. J Bacteriol. 1941 Feb;41(2):109–126. [PMC free article] [PubMed]
  • Hirai Y. Survival of bacteria under dry conditions; from a viewpoint of nosocomial infection. J Hosp Infect. 1991 Nov;19(3):191–200. [PubMed]
  • Horrevorts A, Bergman K, Kollée L, Breuker I, Tjernberg I, Dijkshoorn L. Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit. J Clin Microbiol. 1995 Jun;33(6):1567–1572. [PMC free article] [PubMed]
  • Jawad A, Hawkey PM, Heritage J, Snelling AM. Description of Leeds Acinetobacter Medium, a new selective and differential medium for isolation of clinically important Acinetobacter spp., and comparison with Herellea agar and Holton's agar. J Clin Microbiol. 1994 Oct;32(10):2353–2358. [PMC free article] [PubMed]
  • Kjelleberg S, Hermansson M, Mårdén P, Jones GW. The transient phase between growth and nongrowth of heterotrophic bacteria, with emphasis on the marine environment. Annu Rev Microbiol. 1987;41:25–49. [PubMed]
  • Matin A. The molecular basis of carbon-starvation-induced general resistance in Escherichia coli. Mol Microbiol. 1991 Jan;5(1):3–10. [PubMed]
  • MCDADE JJ, HALL LB. SURVIVAL OF GRAM-NEGATIVE BACTERIA IN THE ENVIRONMENT. I. EFFECT OF RELATIVE HUMIDITY ON SURFACE-EXPOSED ORGANISMS. Am J Hyg. 1964 Sep;80:192–204. [PubMed]
  • Musa EK, Desai N, Casewell MW. The survival of Acinetobacter calcoaceticus inoculated on fingertips and on formica. J Hosp Infect. 1990 Apr;15(3):219–227. [PubMed]
  • Nordström K, Norbäck D, Akselsson R. Effect of air humidification on the sick building syndrome and perceived indoor air quality in hospitals: a four month longitudinal study. Occup Environ Med. 1994 Oct;51(10):683–688. [PMC free article] [PubMed]
  • Nyström T, Olsson RM, Kjelleberg S. Survival, stress resistance, and alterations in protein expression in the marine vibrio sp. strain S14 during starvation for different individual nutrients. Appl Environ Microbiol. 1992 Jan;58(1):55–65. [PMC free article] [PubMed]
  • Patterson JE, Vecchio J, Pantelick EL, Farrel P, Mazon D, Zervos MJ, Hierholzer WJ., Jr Association of contaminated gloves with transmission of Acinetobacter calcoaceticus var. anitratus in an intensive care unit. Am J Med. 1991 Nov;91(5):479–483. [PubMed]
  • Pettit F, Lowbury EJ. Survival of wound pathogens under different environmental conditions. J Hyg (Lond) 1968 Sep;66(3):393–406. [PMC free article] [PubMed]
  • Potts M. Desiccation tolerance of prokaryotes. Microbiol Rev. 1994 Dec;58(4):755–805. [PMC free article] [PubMed]
  • Reindersma P, Nohlmans L, Korten JJ. Acinetobacter, an infrequent cause of community acquired bacterial meningitis. Clin Neurol Neurosurg. 1993 Mar;95(1):71–73. [PubMed]
  • Retailliau HF, Hightower AW, Dixon RE, Allen JR. Acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern. J Infect Dis. 1979 Mar;139(3):371–375. [PubMed]
  • Seifert H, Baginski R, Schulze A, Pulverer G. The distribution of Acinetobacter species in clinical culture materials. Zentralbl Bakteriol. 1993 Nov;279(4):544–552. [PubMed]
  • Sherertz RJ, Sullivan ML. An outbreak of infections with Acinetobacter calcoaceticus in burn patients: contamination of patients' mattresses. J Infect Dis. 1985 Feb;151(2):252–258. [PubMed]
  • Siau H, Yuen KY, Wong SS, Ho PL, Luk WK. The epidemiology of acinetobacter infections in Hong Kong. J Med Microbiol. 1996 May;44(5):340–347. [PubMed]
  • Smith PW, Massanari RM. Room humidifiers as the source of Acinetobacter infections. JAMA. 1977 Feb 21;237(8):795–797. [PubMed]
  • Spector MP, Park YK, Tirgari S, Gonzalez T, Foster JW. Identification and characterization of starvation-regulated genetic loci in Salmonella typhimurium by using Mu d-directed lacZ operon fusions. J Bacteriol. 1988 Jan;170(1):345–351. [PMC free article] [PubMed]
  • Vaneechoutte M, Dijkshoorn L, Tjernberg I, Elaichouni A, de Vos P, Claeys G, Verschraegen G. Identification of Acinetobacter genomic species by amplified ribosomal DNA restriction analysis. J Clin Microbiol. 1995 Jan;33(1):11–15. [PMC free article] [PubMed]
  • Washington JA. An international multicenter study of blood culture practices. The International Collaborative Blood Culture Study Group. Eur J Clin Microbiol Infect Dis. 1992 Dec;11(12):1115–1128. [PubMed]
  • Weernink A, Severin WP, Tjernberg I, Dijkshoorn L. Pillows, an unexpected source of Acinetobacter. J Hosp Infect. 1995 Mar;29(3):189–199. [PubMed]

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