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J Clin Microbiol. Nov 1996; 34(11): 2843–2846.
PMCID: PMC229418

Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex.


The specificity of IS6110 for the Mycobacterium tuberculosis complex has recently been questioned. We observed no cross-reaction with 27 nontuberculous mycobacteria using strand displacement- and PCR-based amplification of the nucleotide 970 to 1026 and 762 to 865 regions of IS6110. These data support use of selected regions of IS6110 as M. tuberculosis complex-specific targets.

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Selected References

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  • Andersen AB, Thybo S, Godfrey-Faussett P, Stoker NG. Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum. Eur J Clin Microbiol Infect Dis. 1993 Dec;12(12):922–927. [PubMed]
  • Clarridge JE, 3rd, Shawar RM, Shinnick TM, Plikaytis BB. Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory. J Clin Microbiol. 1993 Aug;31(8):2049–2056. [PMC free article] [PubMed]
  • Desjardin LE, Perkins MD, Teixeira L, Cave MD, Eisenach KD. Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA. J Clin Microbiol. 1996 Oct;34(10):2435–2439. [PMC free article] [PubMed]
  • Down JA, O'Connell MA, Dey MS, Walters AH, Howard DR, Little MC, Keating WE, Zwadyk P, Jr, Haaland PD, McLaurin DA, 3rd, et al. Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA. J Clin Microbiol. 1996 Apr;34(4):860–865. [PMC free article] [PubMed]
  • Eisenach KD, Cave MD, Bates JH, Crawford JT. Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis. 1990 May;161(5):977–981. [PubMed]
  • Eisenach KD, Sifford MD, Cave MD, Bates JH, Crawford JT. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am Rev Respir Dis. 1991 Nov;144(5):1160–1163. [PubMed]
  • Hellyer TJ, Fletcher TW, Bates JH, Stead WW, Templeton GL, Cave MD, Eisenach KD. Strand displacement amplification and the polymerase chain reaction for monitoring response to treatment in patients with pulmonary tuberculosis. J Infect Dis. 1996 Apr;173(4):934–941. [PubMed]
  • Iovannisci DM, Winn-Deen ES. Ligation amplification and fluorescence detection of Mycobacterium tuberculosis DNA. Mol Cell Probes. 1993 Feb;7(1):35–43. [PubMed]
  • Kent L, McHugh TD, Billington O, Dale JW, Gillespie SH. Demonstration of homology between IS6110 of Mycobacterium tuberculosis and DNAs of other Mycobacterium spp.? J Clin Microbiol. 1995 Sep;33(9):2290–2293. [PMC free article] [PubMed]
  • Kox LF, Rhienthong D, Miranda AM, Udomsantisuk N, Ellis K, van Leeuwen J, van Heusden S, Kuijper S, Kolk AH. A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples. J Clin Microbiol. 1994 Mar;32(3):672–678. [PMC free article] [PubMed]
  • Longo MC, Berninger MS, Hartley JL. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 1990 Sep 1;93(1):125–128. [PubMed]
  • Nolte FS, Metchock B, McGowan JE, Jr, Edwards A, Okwumabua O, Thurmond C, Mitchell PS, Plikaytis B, Shinnick T. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization. J Clin Microbiol. 1993 Jul;31(7):1777–1782. [PMC free article] [PubMed]
  • Noordhoek GT, Kolk AH, Bjune G, Catty D, Dale JW, Fine PE, Godfrey-Faussett P, Cho SN, Shinnick T, Svenson SB, et al. Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories. J Clin Microbiol. 1994 Feb;32(2):277–284. [PMC free article] [PubMed]
  • Pietrzak J, Frei R, Senn HP, Moroni C. Comparison of polymerase chain reaction with standard methods in the diagnosis of Mycobacterium tuberculosis infection. Eur J Clin Microbiol Infect Dis. 1994 Dec;13(12):1079–1083. [PubMed]
  • Querol JM, Farga MA, Granda D, Gimeno C, García-de-Lomas J. The utility of polymerase chain reaction (PCR) in the diagnosis of pulmonary tuberculosis. Chest. 1995 Jun;107(6):1631–1635. [PubMed]
  • Rish JA, Eisenach KD, Cave MD, Reddy MV, Gangadharam PR, Bates JH. Polymerase chain reaction detection of Mycobacterium tuberculosis in formalin-fixed tissue. Am J Respir Crit Care Med. 1996 Apr;153(4 Pt 1):1419–1423. [PubMed]
  • Savić B, Sjöbring U, Alugupalli S, Larsson L, Miörner H. Evaluation of polymerase chain reaction, tuberculostearic acid analysis, and direct microscopy for the detection of Mycobacterium tuberculosis in sputum. J Infect Dis. 1992 Nov;166(5):1177–1180. [PubMed]
  • Schluger NW, Kinney D, Harkin TJ, Rom WN. Clinical utility of the polymerase chain reaction in the diagnosis of infections due to Mycobacterium tuberculosis. Chest. 1994 Apr;105(4):1116–1121. [PubMed]
  • Shawar RM, el-Zaatari FA, Nataraj A, Clarridge JE. Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods. J Clin Microbiol. 1993 Jan;31(1):61–65. [PMC free article] [PubMed]
  • Spargo CA, Haaland PD, Jurgensen SR, Shank DD, Walker GT. Chemiluminescent detection of strand displacement amplified DNA from species comprising the Mycobacterium tuberculosis complex. Mol Cell Probes. 1993 Oct;7(5):395–404. [PubMed]
  • Thierry D, Brisson-Noël A, Vincent-Lévy-Frébault V, Nguyen S, Guesdon JL, Gicquel B. Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis. J Clin Microbiol. 1990 Dec;28(12):2668–2673. [PMC free article] [PubMed]
  • Thierry D, Cave MD, Eisenach KD, Crawford JT, Bates JH, Gicquel B, Guesdon JL. IS6110, an IS-like element of Mycobacterium tuberculosis complex. Nucleic Acids Res. 1990 Jan 11;18(1):188–188. [PMC free article] [PubMed]
  • Walker GT, Fraiser MS, Schram JL, Little MC, Nadeau JG, Malinowski DP. Strand displacement amplification--an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 1992 Apr 11;20(7):1691–1696. [PMC free article] [PubMed]
  • Walker GT, Little MC, Nadeau JG, Shank DD. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):392–396. [PMC free article] [PubMed]
  • Wilson SM, McNerney R, Nye PM, Godfrey-Faussett PD, Stoker NG, Voller A. Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis. J Clin Microbiol. 1993 Apr;31(4):776–782. [PMC free article] [PubMed]

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