Logo of jcellbiolHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Cell Biol. 1994 Jun 2; 125(6): 1201–1212.
PMCID: PMC2290916

Dynamic properties of nuclear lamins: lamin B is associated with sites of DNA replication


The nuclear lamins form a fibrous structure, the nuclear lamina, at the periphery of the nucleus. Recent results suggest that lamins are also present as foci or spots in the nucleoplasm at various times during interphase of the cell cycle (Goldman, A. E., R. D. Moir, M. Montag- Lowy, M. Stewart, and R. D. Goldman. 1992. J. Cell Biol. 104:725-732; Bridger, J. M., I. R. Kill, M. O'Farrell, and C. J. Hutchison. 1993. J. Cell Sci. 104:297-306). In this report we demonstrate that during mid- late S-phase, nuclear foci detected with lamin B antibodies are coincident with sites of DNA replication as detected by the colocalization of sites of incorporation of bromodeoxyuridine (BrDU) or proliferating cell nuclear antigen (PCNA). The relationship between lamin B and BrDU is not maintained in the following G1 stage of the cell cycle. Furthermore, the nuclear staining patterns seen with antibodies directed against lamins A and C in mid-late S-phase do not coalign with the lamin B/BrDU-containing structures. These results imply that there is a role for lamin B in the organization of replicating chromatin during S phase.

Full Text

The Full Text of this article is available as a PDF (4.9M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aebi U, Cohn J, Buhle L, Gerace L. The nuclear lamina is a meshwork of intermediate-type filaments. Nature. 1986 Oct 9;323(6088):560–564. [PubMed]
  • Belmont AS, Zhai Y, Thilenius A. Lamin B distribution and association with peripheral chromatin revealed by optical sectioning and electron microscopy tomography. J Cell Biol. 1993 Dec;123(6 Pt 2):1671–1685. [PMC free article] [PubMed]
  • Bridger JM, Kill IR, O'Farrell M, Hutchison CJ. Internal lamin structures within G1 nuclei of human dermal fibroblasts. J Cell Sci. 1993 Feb;104(Pt 2):297–306. [PubMed]
  • Burke B, Gerace L. A cell free system to study reassembly of the nuclear envelope at the end of mitosis. Cell. 1986 Feb 28;44(4):639–652. [PubMed]
  • Collard JF, Senécal JL, Raymond Y. Redistribution of nuclear lamin A is an early event associated with differentiation of human promyelocytic leukemia HL-60 cells. J Cell Sci. 1992 Mar;101(Pt 3):657–670. [PubMed]
  • Dagenais A, Bibor-Hardy V, Simard R. Characterization of lamin proteins in BHK cells. Exp Cell Res. 1984 Dec;155(2):435–447. [PubMed]
  • Dessev G, Palazzo R, Rebhun L, Goldman R. Disassembly of the nuclear envelope of spisula oocytes in a cell-free system. Dev Biol. 1989 Feb;131(2):496–504. [PubMed]
  • Fields AP, Pettit GR, May WS. Phosphorylation of lamin B at the nuclear membrane by activated protein kinase C. J Biol Chem. 1988 Jun 15;263(17):8253–8260. [PubMed]
  • Foisner R, Gerace L. Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell. 1993 Jul 2;73(7):1267–1279. [PubMed]
  • Foisy S, Bibor-Hardy V. Synthesis of nuclear lamins in BHK-21 cells synchronized with aphidicolin. Biochem Biophys Res Commun. 1988 Oct 14;156(1):205–210. [PubMed]
  • Fox MH, Arndt-Jovin DJ, Jovin TM, Baumann PH, Robert-Nicoud M. Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts. J Cell Sci. 1991 Jun;99(Pt 2):247–253. [PubMed]
  • Fu XD, Maniatis T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature. 1990 Feb 1;343(6257):437–441. [PubMed]
  • Gerace L, Burke B. Functional organization of the nuclear envelope. Annu Rev Cell Biol. 1988;4:335–374. [PubMed]
  • Gerace L, Comeau C, Benson M. Organization and modulation of nuclear lamina structure. J Cell Sci Suppl. 1984;1:137–160. [PubMed]
  • Goldman AE, Moir RD, Montag-Lowy M, Stewart M, Goldman RD. Pathway of incorporation of microinjected lamin A into the nuclear envelope. J Cell Biol. 1992 Nov;119(4):725–735. [PMC free article] [PubMed]
  • Heald R, McKeon F. Mutations of phosphorylation sites in lamin A that prevent nuclear lamina disassembly in mitosis. Cell. 1990 May 18;61(4):579–589. [PubMed]
  • Hennekes H, Peter M, Weber K, Nigg EA. Phosphorylation on protein kinase C sites inhibits nuclear import of lamin B2. J Cell Biol. 1993 Mar;120(6):1293–1304. [PMC free article] [PubMed]
  • Holtz D, Tanaka RA, Hartwig J, McKeon F. The CaaX motif of lamin A functions in conjunction with the nuclear localization signal to target assembly to the nuclear envelope. Cell. 1989 Dec 22;59(6):969–977. [PubMed]
  • Kaufmann SH. Additional members of the rat liver lamin polypeptide family. Structural and immunological characterization. J Biol Chem. 1989 Aug 15;264(23):13946–13955. [PubMed]
  • Kill IR, Bridger JM, Campbell KH, Maldonado-Codina G, Hutchison CJ. The timing of the formation and usage of replicase clusters in S-phase nuclei of human diploid fibroblasts. J Cell Sci. 1991 Dec;100(Pt 4):869–876. [PubMed]
  • Kitten GT, Nigg EA. The CaaX motif is required for isoprenylation, carboxyl methylation, and nuclear membrane association of lamin B2. J Cell Biol. 1991 Apr;113(1):13–23. [PMC free article] [PubMed]
  • Krohne G, Waizenegger I, Höger TH. The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope. J Cell Biol. 1989 Nov;109(5):2003–2011. [PMC free article] [PubMed]
  • Lamb NJ, Cavadore JC, Labbe JC, Maurer RA, Fernandez A. Inhibition of cAMP-dependent protein kinase plays a key role in the induction of mitosis and nuclear envelope breakdown in mammalian cells. EMBO J. 1991 Jun;10(6):1523–1533. [PMC free article] [PubMed]
  • Lehner CF, Kurer V, Eppenberger HM, Nigg EA. The nuclear lamin protein family in higher vertebrates. Identification of quantitatively minor lamin proteins by monoclonal antibodies. J Biol Chem. 1986 Oct 5;261(28):13293–13301. [PubMed]
  • Leonhardt H, Page AW, Weier HU, Bestor TH. A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei. Cell. 1992 Nov 27;71(5):865–873. [PubMed]
  • Lourim D, Lin JJ. Expression of wild-type and nuclear localization-deficient human lamin A in chick myogenic cells. J Cell Sci. 1992 Nov;103(Pt 3):863–874. [PubMed]
  • Lourim D, Krohne G. Membrane-associated lamins in Xenopus egg extracts: identification of two vesicle populations. J Cell Biol. 1993 Nov;123(3):501–512. [PMC free article] [PubMed]
  • Ludérus ME, de Graaf A, Mattia E, den Blaauwen JL, Grande MA, de Jong L, van Driel R. Binding of matrix attachment regions to lamin B1. Cell. 1992 Sep 18;70(6):949–959. [PubMed]
  • Lüscher B, Brizuela L, Beach D, Eisenman RN. A role for the p34cdc2 kinase and phosphatases in the regulation of phosphorylation and disassembly of lamin B2 during the cell cycle. EMBO J. 1991 Apr;10(4):865–875. [PMC free article] [PubMed]
  • Lutz RJ, Trujillo MA, Denham KS, Wenger L, Sinensky M. Nucleoplasmic localization of prelamin A: implications for prenylation-dependent lamin A assembly into the nuclear lamina. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):3000–3004. [PMC free article] [PubMed]
  • Mancini MA, Shan B, Nickerson JA, Penman S, Lee WH. The retinoblastoma gene product is a cell cycle-dependent, nuclear matrix-associated protein. Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):418–422. [PMC free article] [PubMed]
  • Meier J, Campbell KH, Ford CC, Stick R, Hutchison CJ. The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts of Xenopus eggs. J Cell Sci. 1991 Mar;98(Pt 3):271–279. [PubMed]
  • Miller RK, Vikstrom K, Goldman RD. Keratin incorporation into intermediate filament networks is a rapid process. J Cell Biol. 1991 May;113(4):843–855. [PMC free article] [PubMed]
  • Moir RD, Goldman RD. Lamin dynamics. Curr Opin Cell Biol. 1993 Jun;5(3):408–411. [PubMed]
  • Moir RD, Donaldson AD, Stewart M. Expression in Escherichia coli of human lamins A and C: influence of head and tail domains on assembly properties and paracrystal formation. J Cell Sci. 1991 Jun;99(Pt 2):363–372. [PubMed]
  • Newport J, Spann T. Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes. Cell. 1987 Jan 30;48(2):219–230. [PubMed]
  • Newport JW, Wilson KL, Dunphy WG. A lamin-independent pathway for nuclear envelope assembly. J Cell Biol. 1990 Dec;111(6 Pt 1):2247–2259. [PMC free article] [PubMed]
  • Nickowitz RE, Worman HJ. Autoantibodies from patients with primary biliary cirrhosis recognize a restricted region within the cytoplasmic tail of nuclear pore membrane glycoprotein Gp210. J Exp Med. 1993 Dec 1;178(6):2237–2242. [PMC free article] [PubMed]
  • Nigg EA. Assembly-disassembly of the nuclear lamina. Curr Opin Cell Biol. 1992 Feb;4(1):105–109. [PubMed]
  • O'Keefe RT, Henderson SC, Spector DL. Dynamic organization of DNA replication in mammalian cell nuclei: spatially and temporally defined replication of chromosome-specific alpha-satellite DNA sequences. J Cell Biol. 1992 Mar;116(5):1095–1110. [PMC free article] [PubMed]
  • Ottaviano Y, Gerace L. Phosphorylation of the nuclear lamins during interphase and mitosis. J Biol Chem. 1985 Jan 10;260(1):624–632. [PubMed]
  • Brinkley BR, Ouspenski I, Zinkowski RP. Structure and molecular organization of the centromere-kinetochore complex. Trends Cell Biol. 1992 Jan;2(1):15–21. [PubMed]
  • Paddy MR, Belmont AS, Saumweber H, Agard DA, Sedat JW. Interphase nuclear envelope lamins form a discontinuous network that interacts with only a fraction of the chromatin in the nuclear periphery. Cell. 1990 Jul 13;62(1):89–106. [PubMed]
  • Peter M, Heitlinger E, Häner M, Aebi U, Nigg EA. Disassembly of in vitro formed lamin head-to-tail polymers by CDC2 kinase. EMBO J. 1991 Jun;10(6):1535–1544. [PMC free article] [PubMed]
  • Pollard KM, Chan EK, Grant BJ, Sullivan KF, Tan EM, Glass CA. In vitro posttranslational modification of lamin B cloned from a human T-cell line. Mol Cell Biol. 1990 May;10(5):2164–2175. [PMC free article] [PubMed]
  • Rosevear ER, McReynolds M, Goldman RD. Dynamic properties of intermediate filaments: disassembly and reassembly during mitosis in baby hamster kidney cells. Cell Motil Cytoskeleton. 1990;17(3):150–166. [PubMed]
  • Steinert PM, Parry DA. Intermediate filaments: conformity and diversity of expression and structure. Annu Rev Cell Biol. 1985;1:41–65. [PubMed]
  • Stewart M. Intermediate filament structure and assembly. Curr Opin Cell Biol. 1993 Feb;5(1):3–11. [PubMed]
  • TODARO GJ, GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. [PMC free article] [PubMed]
  • Vourc'h C, Taruscio D, Boyle AL, Ward DC. Cell cycle-dependent distribution of telomeres, centromeres, and chromosome-specific subsatellite domains in the interphase nucleus of mouse lymphocytes. Exp Cell Res. 1993 Mar;205(1):142–151. [PubMed]
  • Ulitzur N, Harel A, Feinstein N, Gruenbaum Y. Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract. J Cell Biol. 1992 Oct;119(1):17–25. [PMC free article] [PubMed]
  • Yang HY, Lieska N, Goldman AE, Goldman RD. A 300,000-mol-wt intermediate filament-associated protein in baby hamster kidney (BHK-21) cells. J Cell Biol. 1985 Feb;100(2):620–631. [PMC free article] [PubMed]
  • Yuan J, Simos G, Blobel G, Georgatos SD. Binding of lamin A to polynucleosomes. J Biol Chem. 1991 May 15;266(14):9211–9215. [PubMed]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Cited in Books
    Cited in Books
    NCBI Bookshelf books that cite the current articles.
  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...