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J Clin Microbiol. Jun 1996; 34(6): 1531–1534.
PMCID: PMC229055

Karyotype instability in Cryptococcus neoformans infection.


The electrophoretic karyotypes of 32 clinical and 3 environmental Cryptococcus neoformans isolates from New York City were studied by contour clamped homogeneous electrophoresis. There was extensive variation among the electrophoretic karyotypes of isolates from different patients. Sequential C. neoformans isolates from patients with chronic or relapsing infection had very similar karyotypes. However, minor differences in electrophoretic karyotypes were detected among sequential isolates from 50% of the patients studied, suggesting the occurrence of chromosomal rearrangements or deletions in vivo. This hypothesis was tested by infecting mice, recovering isolates from mouse organs, and comparing the electrophoretic karyotypes before and after passage. Three clinical and three environmental strains were studied before and after passage in mice. Karyotype differences were detected after mouse passage for one clinical and two environmental strains. Our results indicate (i) extensive karyotype variation among isolates from a small geographic regions, (ii) a high frequency of electrophoretic karyotype differences among sequential isolates from individual patients, and (iii) the occurrence of electrophoretic karyotype changes during experimental infection of mice. The implications of these observations are discussed.

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Selected References

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  • Brandt ME, Hutwagner LC, Klug LA, Baughman WS, Rimland D, Graviss EA, Hamill RJ, Thomas C, Pappas PG, Reingold AL, et al. Molecular subtype distribution of Cryptococcus neoformans in four areas of the United States. Cryptococcal Disease Active Surveillance Group. J Clin Microbiol. 1996 Apr;34(4):912–917. [PMC free article] [PubMed]
  • Brandt ME, Hutwagner LC, Kuykendall RJ, Pinner RW. Comparison of multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis for molecular subtyping of Cryptococcus neoformans. The Cryplococcal Disease Active Surveillance Group. J Clin Microbiol. 1995 Jul;33(7):1890–1895. [PMC free article] [PubMed]
  • Casadevall A, Freundlich LF, Marsh L, Scharff MD. Extensive allelic variation in Cryptococcus neoformans. J Clin Microbiol. 1992 May;30(5):1080–1084. [PMC free article] [PubMed]
  • Cherniak R, Morris LC, Belay T, Spitzer ED, Casadevall A. Variation in the structure of glucuronoxylomannan in isolates from patients with recurrent cryptococcal meningitis. Infect Immun. 1995 May;63(5):1899–1905. [PMC free article] [PubMed]
  • Corcoran LM, Forsyth KP, Bianco AE, Brown GV, Kemp DJ. Chromosome size polymorphisms in Plasmodium falciparum can involve deletions and are frequent in natural parasite populations. Cell. 1986 Jan 17;44(1):87–95. [PubMed]
  • Currie B, Sanati H, Ibrahim AS, Edwards JE, Jr, Casadevall A, Ghannoum MA. Sterol compositions and susceptibilities to amphotericin B of environmental Cryptococcus neoformans isolates are changed by murine passage. Antimicrob Agents Chemother. 1995 Sep;39(9):1934–1937. [PMC free article] [PubMed]
  • Currie BP, Casadevall A. Estimation of the prevalence of cryptococcal infection among patients infected with the human immunodeficiency virus in New York City. Clin Infect Dis. 1994 Dec;19(6):1029–1033. [PubMed]
  • Currie BP, Freundlich LF, Casadevall A. Restriction fragment length polymorphism analysis of Cryptococcus neoformans isolates from environmental (pigeon excreta) and clinical sources in New York City. J Clin Microbiol. 1994 May;32(5):1188–1192. [PMC free article] [PubMed]
  • Dromer F, Varma A, Ronin O, Mathoulin S, Dupont B. Molecular typing of Cryptococcus neoformans serotype D clinical isolates. J Clin Microbiol. 1994 Oct;32(10):2364–2371. [PMC free article] [PubMed]
  • Ellis DH, Pfeiffer TJ. Ecology, life cycle, and infectious propagule of Cryptococcus neoformans. Lancet. 1990 Oct 13;336(8720):923–925. [PubMed]
  • Kwon-Chung KJ, Wickes BL, Stockman L, Roberts GD, Ellis D, Howard DH. Virulence, serotype, and molecular characteristics of environmental strains of Cryptococcus neoformans var. gattii. Infect Immun. 1992 May;60(5):1869–1874. [PMC free article] [PubMed]
  • Magee JT, Philpot C, Yang J, Hosein IK. Pyrolysis typing of isolates from a recurrence of systemic cryptococcosis. J Med Microbiol. 1994 Mar;40(3):165–169. [PubMed]
  • Meyer W, Mitchell TG, Freedman EZ, Vilgalys R. Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. J Clin Microbiol. 1993 Sep;31(9):2274–2280. [PMC free article] [PubMed]
  • Perfect JR, Ketabchi N, Cox GM, Ingram CW, Beiser CL. Karyotyping of Cryptococcus neoformans as an epidemiological tool. J Clin Microbiol. 1993 Dec;31(12):3305–3309. [PMC free article] [PubMed]
  • Perfect JR, Magee BB, Magee PT. Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis. Infect Immun. 1989 Sep;57(9):2624–2627. [PMC free article] [PubMed]
  • Polacheck I, Lebens GA. Electrophoretic karyotype of the pathogenic yeast Cryptococcus neoformans. J Gen Microbiol. 1989 Jan;135(1):65–71. [PubMed]
  • Powderly WG. Therapy for cryptococcal meningitis in patients with AIDS. Clin Infect Dis. 1992 Mar;14 (Suppl 1):S54–S59. [PubMed]
  • Ramsey H, Morrow B, Soll DR. An increase in switching frequency correlates with an increase in recombination of the ribosomal chromosomes of Candida albicans strain 3153A. Microbiology. 1994 Jul;140(Pt 7):1525–1531. [PubMed]
  • Spitzer ED, Spitzer SG. Use of a dispersed repetitive DNA element to distinguish clinical isolates of Cryptococcus neoformans. J Clin Microbiol. 1992 May;30(5):1094–1097. [PMC free article] [PubMed]
  • Spitzer ED, Spitzer SG, Freundlich LF, Casadevall A. Persistence of initial infection in recurrent Cryptococcus neoformans meningitis. Lancet. 1993 Mar 6;341(8845):595–596. [PubMed]
  • Varma A, Kwon-Chung KJ. Restriction fragment polymorphism in mitochondrial DNA of Cryptococcus neoformans. J Gen Microbiol. 1989 Dec;135(12):3353–3362. [PubMed]
  • Varma A, Kwon-Chung KJ. DNA probe for strain typing of Cryptococcus neoformans. J Clin Microbiol. 1992 Nov;30(11):2960–2967. [PMC free article] [PubMed]
  • Varma A, Swinne D, Staib F, Bennett JE, Kwon-Chung KJ. Diversity of DNA fingerprints in Cryptococcus neoformans. J Clin Microbiol. 1995 Jul;33(7):1807–1814. [PMC free article] [PubMed]
  • Wickes BL, Moore TD, Kwon-Chung KJ. Comparison of the electrophoretic karyotypes and chromosomal location of ten genes in the two varieties of Cryptococcus neoformans. Microbiology. 1994 Mar;140(Pt 3):543–550. [PubMed]
  • Zolan ME. Chromosome-length polymorphism in fungi. Microbiol Rev. 1995 Dec;59(4):686–698. [PMC free article] [PubMed]

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