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J Cell Biol. Jul 2, 1992; 118(2): 467–479.
PMCID: PMC2290055

Domain-specific and cell type-specific localization of two types of cell wall matrix polysaccharides in the clover root tip

Abstract

Using immunocytochemical techniques and antibodies that specifically recognize xyloglucan (anti-XG), polygalacturonic acid/rhamnogalacturonan I (anti-PGA/RG-I), and methylesterified pectins (JIM 7), we have shown that these polysaccharides are differentially synthesized and localized during cell development and differentiation in the clover root tip. In cortical cells XG epitopes are present at a threefold greater density in the newly formed cross walls than in the older longitudinal walls, and PGA/RG-I epitopes are detected solely in the expanded middle lamella of cortical cell corners, even after pretreatment of sections with pectinmethylesterase to uncover masked epitopes. These results suggest that in cortical cells XG and PGA/RG-I are differentially localized not only to particular wall domains, but also to particular cell walls. In contrast to their nonoverlapping distribution in cortical cells, XG epitopes and PGA/RG-I epitopes largely colocalize in the epidermal cell walls. The results also demonstrate that the middle lamella of the longitudinal walls shared by epidermal cells and by epidermal and cortical cells constitutes a barrier to the diffusion of cell wall and mucilage molecules. Synthesis of XG and PGA/RG-I epitope-containing polysaccharides also varies during cellular differentiation in the root cap. The differentiation of gravitropic columella cells into mucilage-secreting peripheral cells is marked by a dramatic increase in the synthesis and secretion of molecules containing XG and PGA/RG-I epitopes. In contrast, JIM 7 epitopes are present at abundant levels in columella cell walls, but are not detectable in peripheral cell walls or in secreted mucilage. There were also changes in the cisternal labeling of the Golgi stacks during cellular differentiation in the root tip. Whereas PGA/RG-I epitopes are detected primarily in cis- and medial Golgi cisternae in cortical cells (Moore, P. J., K. M. M. Swords, M. A. Lynch, and L. A. Staehelin. 1991. J. Cell Biol. 112:589-602), they are localized predominantly in the trans-Golgi cisternae and the trans-Golgi network in epidermal and peripheral root cap cells. These observations suggest that during cellular differentiation the plant Golgi apparatus can be both structurally and functionally reorganized.

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Selected References

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  • Bauer WD, Talmadge KW, Keegstra K, Albersheim P. The Structure of Plant Cell Walls: II. The Hemicellulose of the Walls of Suspension-cultured Sycamore Cells. Plant Physiol. 1973 Jan;51(1):174–187. [PMC free article] [PubMed]
  • Eberhard S, Doubrava N, Marfa V, Mohnen D, Southwick A, Darvill A, Albersheim P. Pectic Cell Wall Fragments Regulate Tobacco Thin-Cell-Layer Explant Morphogenesis. Plant Cell. 1989 Aug;1(8):747–755. [PMC free article] [PubMed]
  • Griffiths G, Pfeiffer S, Simons K, Matlin K. Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane. J Cell Biol. 1985 Sep;101(3):949–964. [PMC free article] [PubMed]
  • Kauss H, Swanson AL, Hassid WZ. Biosynthesis of the methyl ester groups of pectin by transmethylation from S-adenosyl-L-methionine. Biochem Biophys Res Commun. 1967 Jan 23;26(2):234–240. [PubMed]
  • Mauch F, Staehelin LA. Functional Implications of the Subcellular Localization of Ethylene-Induced Chitinase and [beta]-1,3-Glucanase in Bean Leaves. Plant Cell. 1989 Apr;1(4):447–457. [PMC free article] [PubMed]
  • McNeil M, Darvill AG, Albersheim P. Structure of Plant Cell Walls: X. RHAMNOGALACTURONAN I, A STRUCTURALLY COMPLEX PECTIC POLYSACCHARIDE IN THE WALLS OF SUSPENSION-CULTURED SYCAMORE CELLS. Plant Physiol. 1980 Dec;66(6):1128–1134. [PMC free article] [PubMed]
  • McNeil M, Darvill AG, Fry SC, Albersheim P. Structure and function of the primary cell walls of plants. Annu Rev Biochem. 1984;53:625–663. [PubMed]
  • Moore PJ, Darvill AG, Albersheim P, Staehelin LA. Immunogold localization of xyloglucan and rhamnogalacturonan I in the cell walls of suspension-cultured sycamore cells. Plant Physiol. 1986 Nov;82(3):787–794. [PMC free article] [PubMed]
  • Moore PJ, Swords KM, Lynch MA, Staehelin LA. Spatial organization of the assembly pathways of glycoproteins and complex polysaccharides in the Golgi apparatus of plants. J Cell Biol. 1991 Feb;112(4):589–602. [PMC free article] [PubMed]
  • Powell DA, Morris ER, Gidley MJ, Rees DA. Conformations and interactions of pectins. II. Influences of residue sequence on chain association in calcium pectate gels. J Mol Biol. 1982 Mar 15;155(4):517–531. [PubMed]
  • Pressey R. Role of Pectinesterase in pH-Dependent Interactions between Pea Cell Wall Polymers. Plant Physiol. 1984 Oct;76(2):547–549. [PMC free article] [PubMed]
  • Roberts K. The plant extracellular matrix. Curr Opin Cell Biol. 1989 Oct;1(5):1020–1027. [PubMed]
  • Roberts K. Structures at the plant cell surface. Curr Opin Cell Biol. 1990 Oct;2(5):920–928. [PubMed]
  • Roth J, Taatjes DJ, Weinstein J, Paulson JC, Greenwell P, Watkins WM. Differential subcompartmentation of terminal glycosylation in the Golgi apparatus of intestinal absorptive and goblet cells. J Biol Chem. 1986 Oct 25;261(30):14307–14312. [PubMed]
  • Staehelin LA, Giddings TH, Jr, Kiss JZ, Sack FD. Macromolecular differentiation of Golgi stacks in root tips of Arabidopsis and Nicotiana seedlings as visualized in high pressure frozen and freeze-substituted samples. Protoplasma. 1990;157(1-3):75–91. [PubMed]
  • Wright K, Northcote DH. The relationship of root-cap slimes to pectins. Biochem J. 1974 Jun;139(3):525–534. [PMC free article] [PubMed]

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