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J Clin Microbiol. 1996 Mar; 34(3): 537–542.
PMCID: PMC228842

Molecular analysis of microflora associated with dentoalveolar abscesses.

Abstract

The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cultured bacteria was used to detect putative unculturable bacteria. Clones representative of five predominant groups of uncultured organisms were sequenced. Two were identified as Porphyromonas gingivalis and Prevotella oris, and one was found to be closely related to Peptostreptococcus micros. The remaining two clones did not correspond to known, previously sequenced organisms. One was related to Zoogloea ramigera, a species of aerobic waterborne organisms, while the other was distantly related to the genus Prevotella. This study has demonstrated the possibility of the characterization of microflora associated with human infection by molecular methods without the inherent biases of culture.

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Selected References

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  • Arank A, Syed SA, Kenney EB, Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. [PMC free article] [PubMed]
  • Brook I, Frazier EH, Gher ME. Aerobic and anaerobic microbiology of periapical abscess. Oral Microbiol Immunol. 1991 Apr;6(2):123–125. [PubMed]
  • Brook I, Friedman EM. Intracranial complications of sinusitis in children. A sequela of periapical abscess. Ann Otol Rhinol Laryngol. 1982 Jan-Feb;91(1 Pt 1):41–43. [PubMed]
  • Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez-Garayzabal J, Garcia P, Cai J, Hippe H, Farrow JA. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol. 1994 Oct;44(4):812–826. [PubMed]
  • Giovannoni SJ, Britschgi TB, Moyer CL, Field KG. Genetic diversity in Sargasso Sea bacterioplankton. Nature. 1990 May 3;345(6270):60–63. [PubMed]
  • Higgins DG, Bleasby AJ, Fuchs R. CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci. 1992 Apr;8(2):189–191. [PubMed]
  • Hollin SA, Hayashi H, Gross SW. Intracranial abscesses of odontogenic origin. Oral Surg Oral Med Oral Pathol. 1967 Mar;23(3):277–293. [PubMed]
  • Lewis MA, MacFarlane TW, McGowan DA. Quantitative bacteriology of acute dento-alveolar abscesses. J Med Microbiol. 1986 Mar;21(2):101–104. [PubMed]
  • Liesack W, Stackebrandt E. Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment. J Bacteriol. 1992 Aug;174(15):5072–5078. [PMC free article] [PubMed]
  • Maidak BL, Larsen N, McCaughey MJ, Overbeek R, Olsen GJ, Fogel K, Blandy J, Woese CR. The Ribosomal Database Project. Nucleic Acids Res. 1994 Sep;22(17):3485–3487. [PMC free article] [PubMed]
  • Oguntebi B, Slee AM, Tanzer JM, Langeland K. Predominant microflora associated with human dental periapical abscesses. J Clin Microbiol. 1982 May;15(5):964–966. [PMC free article] [PubMed]
  • Relman DA, Loutit JS, Schmidt TM, Falkow S, Tompkins LS. The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens. N Engl J Med. 1990 Dec 6;323(23):1573–1580. [PubMed]
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. [PubMed]
  • SOCRANSKY SS, GIBBONS RJ, DALE AC, BORTNICK L, ROSENTHAL E, MACDONALD JB. The microbiota of the gingival crevice area of man. I. Total microscopic and viable counts and counts of specific organisms. Arch Oral Biol. 1963 May-Jun;8:275–280. [PubMed]
  • Wade WG, Gray AR, Absi EG, Barker GR. Predominant cultivable flora in pericoronitis. Oral Microbiol Immunol. 1991 Oct;6(5):310–312. [PubMed]
  • Wade WG, Lewis MA, Cheeseman SL, Absi EG, Bishop PA. An unclassified Eubacterium taxon in acute dento-alveolar abscess. J Med Microbiol. 1994 Feb;40(2):115–117. [PubMed]
  • Ward DM, Weller R, Bateson MM. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature. 1990 May 3;345(6270):63–65. [PubMed]
  • Wilson KH, Blitchington R, Frothingham R, Wilson JA. Phylogeny of the Whipple's-disease-associated bacterium. Lancet. 1991 Aug 24;338(8765):474–475. [PubMed]

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