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J Clin Microbiol. Feb 1996; 34(2): 450–453.
PMCID: PMC228819

Detection of Mycobacterium tuberculosis in cerebrospinal fluid following immunomagnetic enrichment.

Abstract

The detection of Mycobacterium tuberculosis by culture of cerebrospinal fluid (CSF) is unacceptably slow. Low numbers of organisms and the presence of reaction inhibitors may prevent detection of M. tuberculosis by PCR. We used immunomagnetic enrichment to accelerate and enhance the detection of mycobacteria in CSF after demonstrating the utility of the method with pure suspensions. Growth was detected earlier in Bactec cultures of magnetically recovered mycobacteria than in untreated CSF (7 versus 15 days). We detected M. tuberculosis DNA by PCR in the immunomagnetically enriched sample but not in untreated CSF. PCR fingerprintings of the immunomagnetically recovered M. tuberculosis and of the isolate subsequently recovered by culture were identical.

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Selected References

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  • Ahuja GK, Mohan KK, Prasad K, Behari M. Diagnostic criteria for tuberculous meningitis and their validation. Tuber Lung Dis. 1994 Apr;75(2):149–152. [PubMed]
  • Bhaduri S, Demchick PH. Simple and rapid method for disruption of bacteria for protein studies. Appl Environ Microbiol. 1983 Oct;46(4):941–943. [PMC free article] [PubMed]
  • Haas WH, Butler WR, Woodley CL, Crawford JT. Mixed-linker polymerase chain reaction: a new method for rapid fingerprinting of isolates of the Mycobacterium tuberculosis complex. J Clin Microbiol. 1993 May;31(5):1293–1298. [PMC free article] [PubMed]
  • Kaneko K, Onodera O, Miyatake T, Tsuji S. Rapid diagnosis of tuberculous meningitis by polymerase chain reaction (PCR). Neurology. 1990 Oct;40(10):1617–1618. [PubMed]
  • Kox LF, Rhienthong D, Miranda AM, Udomsantisuk N, Ellis K, van Leeuwen J, van Heusden S, Kuijper S, Kolk AH. A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples. J Clin Microbiol. 1994 Mar;32(3):672–678. [PMC free article] [PubMed]
  • Lee BW, Tan JA, Wong SC, Tan CB, Yap HK, Low PS, Chia JN, Tay JS. DNA amplification by the polymerase chain reaction for the rapid diagnosis of tuberculous meningitis. Comparison of protocols involving three mycobacterial DNA sequences, IS6110, 65 kDa antigen, and MPB64. J Neurol Sci. 1994 May;123(1-2):173–179. [PubMed]
  • Liu PY, Shi ZY, Lau YJ, Hu BS. Rapid diagnosis of tuberculous meningitis by a simplified nested amplification protocol. Neurology. 1994 Jun;44(6):1161–1164. [PubMed]
  • Meyers BR. Tuberculous meningitis. Med Clin North Am. 1982 May;66(3):755–762. [PubMed]
  • Molavi A, LeFrock JL. Tuberculous meningitis. Med Clin North Am. 1985 Mar;69(2):315–331. [PubMed]
  • Olsvik O, Popovic T, Skjerve E, Cudjoe KS, Hornes E, Ugelstad J, Uhlén M. Magnetic separation techniques in diagnostic microbiology. Clin Microbiol Rev. 1994 Jan;7(1):43–54. [PMC free article] [PubMed]
  • Rys PN, Persing DH. Preventing false positives: quantitative evaluation of three protocols for inactivation of polymerase chain reaction amplification products. J Clin Microbiol. 1993 Sep;31(9):2356–2360. [PMC free article] [PubMed]
  • Shankar P, Manjunath N, Mohan KK, Prasad K, Behari M, Shriniwas, Ahuja GK. Rapid diagnosis of tuberculous meningitis by polymerase chain reaction. Lancet. 1991 Jan 5;337(8732):5–7. [PubMed]
  • Weston PD, Avrameas S. Proteins coupled to polyacrylamide beads using glutaraldehyde. Biochem Biophys Res Commun. 1971 Dec 17;45(6):1574–1580. [PubMed]
  • Widjojoatmodjo MN, Fluit AC, Torensma R, Verdonk GP, Verhoef J. The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samples. J Clin Microbiol. 1992 Dec;30(12):3195–3199. [PMC free article] [PubMed]
  • Wolfhagen MJ, Fluit AC, Torensma R, Poppelier MJ, Verhoef J. Rapid detection of toxigenic Clostridium difficile in fecal samples by magnetic immuno PCR assay. J Clin Microbiol. 1994 Jul;32(7):1629–1633. [PMC free article] [PubMed]

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