• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Oct 1995; 33(10): 2576–2581.
PMCID: PMC228531

Characterization of Streptococcus agalactiae strains by multilocus enzyme genotype and serotype: identification of multiple virulent clone families that cause invasive neonatal disease.


The chromosomal genotypes of 277 isolates of 16 serotypes of Streptococcus agalactiae were characterized by analysis of electrophoretically demonstrable allele profiles at 12 metabolic enzyme loci. The collection comprised the type strain and 276 strains recovered from French symptomatic and asymptomatic subjects. Sixty-one distinctive electrophoretic types (ETs), representing multilocus clonal genotypes, were identified. Cluster analysis of the ETs revealed two primary phylogenetic divisions separated by a genetic distance of 0.62, Division I contained 67 isolates which could be assigned to 13 ETs. Twenty-seven of these isolates were from samples of cerebrospinal fluid (CSF) from neonatal meningitis patients. Two ETs, separated by a genetic distance of 0.217, contained 26 of these 27 isolates. Division II contained 210 isolates, of which 27 were isolated from CSF. This division was more polymorphic and included 48 ETs. Spanning a genetic distance of 0.3, three clusters and one ET were identified within this group. Twenty-four of 27 strains isolated from CSF belonged to one cluster, and 19 of them belonged to two adjacent ETs with a genetic distance of 0.083. Fifty-five of the 68 serotype Ia strains and 24 of the 26 serotype Ib strains were each confined to one of the evolutionary lineages, and 85 of the 86 strains which carried protein antigen c belonged to phylogenetic division II. Most of the type III organisms were assigned to two clone families. The characteristics of this French population argue for the existence of particular groups of strains responsible for neonatal meningitis and demonstrate that serotyping can supply information about the genetic distribution of strains.

Full Text

The Full Text of this article is available as a PDF (199K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anthony BF, Okada DM. The emergence of group B streptococci in infections of the newborn infant. Annu Rev Med. 1977;28:355–369. [PubMed]
  • Blond MH, Gold F, Quentin R, Legare C, Pierre F, Borderon JC, Laugier J. Infection bactérienne du nouveau-né par contamination materno-foetale: on peut se fier à l'anamnèse. J Gynecol Obstet Biol Reprod (Paris) 1992;21(4):393–397. [PubMed]
  • Blond MH, Gold F, Quentin R, Pierre F, Kompanietz J, Soutoul JH, Laugier J. Infection bactérienne du nouveau-né par contamination materno-foetale. Etude épidémiologique rétrospective dans une maternité. J Gynecol Obstet Biol Reprod (Paris) 1991;20(3):443–446. [PubMed]
  • Blumberg HM, Stephens DS, Licitra C, Pigott N, Facklam R, Swaminathan B, Wachsmuth IK. Molecular epidemiology of group B streptococcal infections: use of restriction endonuclease analysis of chromosomal DNA and DNA restriction fragment length polymorphisms of ribosomal RNA genes (ribotyping). J Infect Dis. 1992 Sep;166(3):574–579. [PubMed]
  • Dillon HC, Jr, Khare S, Gray BM. Group B streptococcal carriage and disease: a 6-year prospective study. J Pediatr. 1987 Jan;110(1):31–36. [PubMed]
  • Feld SM, Harrigan JT. Vaginal gram stain as an immediate detector of group B streptococci in selected obstetric patients. Am J Obstet Gynecol. 1987 Feb;156(2):446–448. [PubMed]
  • Ferrieri P. Surface-localized protein antigens of group B streptococci. Rev Infect Dis. 1988 Jul-Aug;10 (Suppl 2):S363–S366. [PubMed]
  • Helmig R, Uldbjerg N, Boris J, Kilian M. Clonal analysis of Streptococcus agalactiae isolated from infants with neonatal sepsis or meningitis and their mothers and from healthy pregnant women. J Infect Dis. 1993 Oct;168(4):904–909. [PubMed]
  • Huet H, Martin C, Geslin P, Grimont F, Quentin R. Ribotyping of Streptococcus agalactiae strains isolated from vaginas of asymptomatic women. Res Microbiol. 1993 Jul-Aug;144(6):457–465. [PubMed]
  • Jelínkoá J. Group B streptococci in the human population. Curr Top Microbiol Immunol. 1977;76:127–165. [PubMed]
  • Jelínková J, Motlová J. Worldwide distribution of two new serotypes of group B streptococci: type IV and provisional type V. J Clin Microbiol. 1985 Mar;21(3):361–362. [PMC free article] [PubMed]
  • Jensen NE. Production and evaluation of antisera for serological type determination of group-B streptococci by double diffusion in agarose gel. Acta Pathol Microbiol Scand B. 1979 Apr;87B(2):77–83. [PubMed]
  • Lancefield RC, McCarty M, Everly WN. Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens. J Exp Med. 1975 Jul 1;142(1):165–179. [PMC free article] [PubMed]
  • Mayon-White RT. The incidence of GBS disease in neonates in different countries. Antibiot Chemother (1971) 1985;35:17–27. [PubMed]
  • Motlová J, Wagner M, Jelínková J. A search for new group-B streptococcal serotypes. J Med Microbiol. 1986 Sep;22(2):101–105. [PubMed]
  • Musser JM, Mattingly SJ, Quentin R, Goudeau A, Selander RK. Identification of a high-virulence clone of type III Streptococcus agalactiae (group B Streptococcus) causing invasive neonatal disease. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4731–4735. [PMC free article] [PubMed]
  • PATTISON IH, MATTHEWS PR, HOWELL DG. The type classification of group-B streptococci, with special reference to bovine strains apparently lacking in type polysaccharide. J Pathol Bacteriol. 1955 Jan-Apr;69(1-2):51–60. [PubMed]
  • Perch B, Kjems E, Henrichsen New serotypes of group B streptococci isolated from human sources. J Clin Microbiol. 1979 Jul;10(1):109–110. [PMC free article] [PubMed]
  • Quentin R, Goudeau A, Wallace RJ, Jr, Smith AL, Selander RK, Musser JM. Urogenital, maternal and neonatal isolates of Haemophilus influenzae: identification of unusually virulent serologically non-typable clone families and evidence for a new Haemophilus species. J Gen Microbiol. 1990 Jul;136(7):1203–1209. [PubMed]
  • Selander RK, Caugant DA, Ochman H, Musser JM, Gilmour MN, Whittam TS. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. [PMC free article] [PubMed]
  • Severin WP. Latex agglutination in the diagnosis of meningococcal meningitis. J Clin Pathol. 1972 Dec;25(12):1079–1082. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...