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J Clin Microbiol. Jun 1995; 33(6): 1640–1642.
PMCID: PMC228233

Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51.

Abstract

Because the brucellosis eradication program uses slaughter and quarantine as control measures, it would benefit from faster methods of bacterial identification. Distinguishing vaccine strains from strains that cause infections among vaccinated herds in the field is essential. To accomplish this, our PCR-based, species-specific assay (B. J. Bricker and S. M. Halling, J. Clin. Microbiol. 32:2660-2666, 1994) was updated to identify Brucella abortus vaccine strains S19 and RB51. Three new oligonucleotide primers were added to the five-primer multiplex Brucella AMOS PCR assay. Identification is based on the number and sizes of six products amplified by PCR.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol. 1994 Nov;32(11):2660–2666. [PMC free article] [PubMed]
  • Cheville NF, Stevens MG, Jensen AE, Tatum FM, Halling SM. Immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of Brucella abortus. Am J Vet Res. 1993 Oct;54(10):1591–1597. [PubMed]
  • Halling SM, Tatum FM, Bricker BJ. Sequence and characterization of an insertion sequence, IS711, from Brucella ovis. Gene. 1993 Oct 29;133(1):123–127. [PubMed]
  • Halling SM, Zehr ES. Polymorphism in Brucella spp. due to highly repeated DNA. J Bacteriol. 1990 Dec;172(12):6637–6640. [PMC free article] [PubMed]
  • JONES LM, MONTGOMERY V, WILSON JB. CHARACTERISTICS OF CARBON DIOXIDE-INDEPENDENT CULTURES OF BRUCELLA ABORTUS ISOLATED FROM CATTLE VACCINATED WITH STRAIN 19. J Infect Dis. 1965 Jun;115:312–320. [PubMed]
  • Sangari FJ, García-Lobo JM, Agüero J. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol Lett. 1994 Sep 1;121(3):337–342. [PubMed]
  • Schurig GG, Roop RM, 2nd, Bagchi T, Boyle S, Buhrman D, Sriranganathan N. Biological properties of RB51; a stable rough strain of Brucella abortus. Vet Microbiol. 1991 Jul;28(2):171–188. [PubMed]
  • Stevens MG, Hennager SG, Olsen SC, Cheville NF. Serologic responses in diagnostic tests for brucellosis in cattle vaccinated with Brucella abortus 19 or RB51. J Clin Microbiol. 1994 Apr;32(4):1065–1066. [PMC free article] [PubMed]
  • Tobias L, Schurig GG, Cordes DO. Comparative behaviour of Brucella abortus strains 19 and RB51 in the pregnant mouse. Res Vet Sci. 1992 Sep;53(2):179–183. [PubMed]

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