• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Mar 1995; 33(3): 636–640.
PMCID: PMC228005

Bacillus cereus phage typing as an epidemiological tool in outbreaks of food poisoning.

Abstract

Bacillus cereus is responsible for an increasing number of food poisoning cases. By using 12 bacteriophages isolated from sewage, a typing scheme for B. cereus isolates from outbreaks or sporadic cases of food poisoning was developed. The phages belonged to three morphotypes. Ten phages with contractile tails and icosahedral heads were members of the Myoviridae family, and two phages with noncontractile tails belonged to the Siphoviridae family. Phage 11 represented a new species. It had an isometric head and a very long contractile tail with long wavy tail fibers and was one of the largest viruses known. The vast majority of 166 B. cereus strains (161, or 97%) isolated from food poisoning cases were typeable. Of 146 strains isolated from 18 outbreaks, 142 (97%) could be divided into 17 phage types. A good correlation, on the order of 80 to 100%, between phage types of strains isolated from suspected foods and those of strains isolated from stools of symptomatic patients was observed. Most Bacillus thuringiensis strains were also typeable, providing further evidence of the close relatedness of B. cereus and B. thuringiensis. This phage typing scheme can be a valuable epidemiological tool in tracing the origins of food poisoning caused by B. cereus.

Full Text

The Full Text of this article is available as a PDF (1.0M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ahmed R, Bopp C, Borczyk A, Kasatiya S. Phage-typing scheme for Escherichia coli O157:H7. J Infect Dis. 1987 Apr;155(4):806–809. [PubMed]
  • Carlson CR, Caugant DA, Kolstø AB. Genotypic Diversity among Bacillus cereus and Bacillus thuringiensis Strains. Appl Environ Microbiol. 1994 Jun;60(6):1719–1725. [PMC free article] [PubMed]
  • Drobniewski FA. Bacillus cereus and related species. Clin Microbiol Rev. 1993 Oct;6(4):324–338. [PMC free article] [PubMed]
  • Hohn T, Hohn B, Engel A, Wurtz M, Smith PR. Isolation and characterization of the host protein groE involved in bacteriophage lambda assembly. J Mol Biol. 1979 Apr 15;129(3):359–373. [PubMed]
  • Jephcott AE, Barton BW, Gilbert RJ, Shearer CW. An unusual outbreak of food-poisoning associated with meals-on-wheels. Lancet. 1977 Jul 16;2(8029):129–130. [PubMed]
  • Luby S, Jones J, Dowda H, Kramer J, Horan J. A large outbreak of gastroenteritis caused by diarrheal toxin-producing Bacillus cereus. J Infect Dis. 1993 Jun;167(6):1452–1455. [PubMed]
  • Mortimer PR, McCann G. Food-poisoning episodes associated with Bacillus cereus in fried rice. Lancet. 1974 May 25;1(7865):1043–1045. [PubMed]
  • Shinagawa K. Analytical methods for Bacillus cereus and other Bacillus species. Int J Food Microbiol. 1990 Mar;10(2):125–141. [PubMed]
  • Taylor AJ, Gilbert RJ. Bacillus cereus food poisoning: a provisional serotyping scheme. J Med Microbiol. 1975 Nov;8(4):543–550. [PubMed]
  • WILLIAMS REO, RIPPON JE. Bacteriophage typing of Staphylococcus aureus. J Hyg (Lond) 1952 Sep;50(3):320–353. [PMC free article] [PubMed]
  • Wong HC, Chang MH, Fan JY. Incidence and characterization of Bacillus cereus isolates contaminating dairy products. Appl Environ Microbiol. 1988 Mar;54(3):699–702. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...