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Proc Natl Acad Sci U S A. Apr 14, 1998; 95(8): 4641–4645.
PMCID: PMC22543
Microbiology

Differential patterns of acquired virulence genes distinguish Salmonella strains

Abstract

Analysis of several Salmonella typhimurium in vivo-induced genes located in regions of atypical base composition has uncovered acquired genetic elements that cumulatively engender pathogenicity. Many of these regions are associated with mobile elements, encode predicted adhesin and invasin-like functions, and are required for full virulence. Some of these regions distinguish broad host range from host-adapted Salmonella serovars and may contribute to inherent differences in host specificity, tissue tropism, and disease manifestation. Maintenance of this archipelago of acquired sequence by selection in specific hosts reveals a fossil record of the evolution of pathogenic species.

Keywords: pathogenesis, horizontal transfer, in vivo expression technology, ivi, Salmonella typhimurium

Microbial speciation is driven by the loss or acquisition of genes that allow an organism to occupy a niche unavailable to its relatives (13). Within the context of pathogenesis, these acquired sequences reflect a pathogen’s unique lifestyle in nature, comprising functions favorable to growth in the host and the environment (4). Many virulence functions that contribute to in vivo fitness are encoded on pathogenicity islands, sequences presumed to have been acquired by horizontal transfer as evidenced by their atypical base composition and codon usage (5). Here we have employed in vivo expression technology (IVET; refs. 6 and 7) to identify in vivo induced (ivi) acquired sequences within Salmonella typhimurium that are distinct within and between Salmonella serovars and contribute to its fitness within its host.

MATERIALS AND METHODS

Bacterial Strains and Phage.

All S. typhimurium strains used in this study were derived from strain ATCC 14028 (CDC 6516–60). The high-frequency generalized-transducing bacteriophage P22 mutant HT 105/1, int-201 was used for all transductional crosses (8), and phage-free, phage-sensitive transductants were isolated as described (9). The isolation of all ivi fusions used in this study was described (10).

Media and Chemicals.

Luria broth (11) was the laboratory media used in these studies. Final concentrations of antibiotics (Sigma) were as follows: ampicillin (50 μg/ml), kanamycin (50 μg/ml), tetracycline (20 μg/ml).

Hybridization Conditions.

Probes used for hybridization were obtained by restriction digestion or amplified by PCR. Typically, 1 μg of genomic DNA was spotted on a nylon membrane and probed overnight with 100 ng of DNA. Membranes were washed at 42°C in 0.5× SSC plus 0.4% SDS and 6 M urea. Hybridization was detected by enhanced chemiluminescence (Amersham).

Competitive Index (CI) Assay in BALB/c Mice.

Mutant and wild-type cells were grown overnight in Luria broth. BALB/c mice were intraperitoneally infected with a total of 103 cells of a 1:1 ratio of deletion mutant: wild type (MT2057). The mice were killed after 5 days and the bacterial cells were recovered from the spleen; the CI is the ratio of the number of mutant/wild-type bacteria recovered.

RESULTS

Serovar-Specific Sequences.

A collection of more than 100 ivi genes (10) was screened for those that reside in regions of atypical base composition (≤49% or ≥57%) and for the absence of sequence homology in the DNA database. A subset of 30 ivi genes that fit these criteria were used as molecular probes to hybridize against genomic DNA isolated from a set of enteric pathogens, including four Salmonella serovars of differing host range, tissue tropism, and disease manifestation.

Table Table11 shows that DNAs prepared from seven of these ivi gene fragments hybridized strongly to genomic DNA prepared from one or more Salmonella serovars and not to any other pathogens tested (with the exception of iviXVII). The Salmonella-specific regions fell into three distinct classes based on their respective hybridization patterns to the four Salmonella serovars tested. These serovars include the broad host range S. typhimurium and Salmonella newport (warm- and cold-blooded animals), limited host range Salmonella choleraesuis (humans, pigs), and the host-adapted Salmonella typhi (humans). Class I sequences hybridized to all Salmonella serovars tested. In contrast, Class II and III sequences were serovar specific: Class II hybridized to all serovars except S. typhi, whereas Class III hybridized only to broad host-range serovars. Control hybridizations show that a typical core sequence marker that is shared between many species, e.g., triose- phosphate isomerase (tpi; ≈80% DNA homology between Salmonella spp. and Escherichia coli), hybridized to all species tested, and the Salmonella plasmid virulence gene, spvB, hybridized only to Salmonella serovars tested that contain the Salmonella virulence plasmid, pSLT (all serovars but S. typhi).

Table 1
ivi sequences that are distinct within and between Salmonella serovars

Salmonella-Specific Regions.

To determine the extent of the chromosomal regions that are distinctive to Salmonella, the ivi gene fragments from these regions were used as hybridization probes against a library of λ clones containing S. typhimurium genomic DNA. Positively hybridizing λ clones were selected and fragments of these (typically 1–3 kb) were used as probes against genomic DNA prepared from the collection of pathogens shown in Table Table1.1. Each line in Fig. Fig.11 represents an assemblage of overlapping λ and/or plasmid clones in a Salmonella-specific region.

Figure 1
Salmonella-specific regions identified by IVET. Each line represents an assemblage of overlapping λ and/or plasmid clones in a Salmonella-specific region. The boxed sequences represent Salmonella-specific regions that are distinct to ...

Fig. Fig.11 shows that three of the Salmonella-specific regions are present in all Salmonella serovars tested (lines 1–3) and are designated Class I. The iviVI-A region (line 1) corresponds to a chromosomal segment of atypical base composition (>35 kb), part of which was known to be present in Salmonella spp., but not in E. coli (10, 21, 22). A second Class I region encodes two ivi fusions, iviXVII and iviXXV (line 2), that reside in a region involved in vitamin B12 biosynthesis (23) and propanediol utilization (pdu) (ref. 24; T. A. Bobik, personal communication). Last, the iviXVIII region (line 3) is comprised of Class I sequences that are separated by a short intervening segment of E. coli-like core sequence.

The remaining three regions have proven to be mosaics of Salmonella serovar-specific sequences (lines 4–6). The iviXIX and iviXX regions (line 4) comprise two distinct blocks of different hybridization classes, Class I and Class III, respectively, separated by a small core sequence. Similarly, the iviXXI region (line 5) comprises two serovar-specific hybridization classes, Class II and Class III. Notably, a portion of the iviXXI region does not hybridize to DNA prepared from the laboratory strain of S. typhimurium, LT2, thus distinguishing not only one serovar from another but also individual strains within a serovar. Last, iviXXII and iviXXIII define an ≈34-kb Class III region that is flanked on either side by Class I sequences (line 6). One of the two flanking Class I regions was previously known to be present in Salmonella spp., but not present in E. coli, containing the flagellar-switch region (hin), and may extend as far as the Salmonella-specific tct locus (25, 26).

Association of Mobile Elements with Salmonella-Specific Acquired Sequences.

Fig. Fig.1,1, line 1 shows a component of an rhs (recombination hot spot) repetitive element, termed an H-repeat (H-rpt), flanks the iviVI-A region on one end (27). This H-rpt insertion-like sequence is present in all Salmonella serovars tested (Class I) and such elements have been associated with the acquisition of virulence functions in several pathogens (2730). Another rhs is also present in this region, defined by iviXXIV. In contrast to the H-rpt, this rhs is only present in broad host-range Salmonella serovars (Class III) and encodes a predicted peptide motif involved in carbohydrate binding (31, 32), suggesting a possible role in adhesion. The portion of the iviVI-A region flanked on both ends by insertion sequences may be inherited as an independent unit.

Several mobile elements reside within the Class III iviXXII region (Fig. (Fig.1,1, line 6). Examples include insertion elements IS3 and IS1351, and homologues to functions encoded on the cryptic prophage, CP4–57. Both Class III junction points of this region are associated with homologues to the CP4–57 integrase, slpA, a homologue that defines the junction of a ToxR-regulated gene cluster in Vibrio cholerae (33). Moreover, one junction point lies near ssrA (encoding 10Sa RNA), a tRNA-like structure that is part of the insertion site for CP4–57 in E. coli (34) and the insertion site of a pathogenicity island in Dichelobacter nodosus (35).

Salmonella-Specific Sequences Contribute to Virulence.

Directed deletions of the Salmonella-specific regions were constructed to determine the overall contribution of these regions to pathogenesis (36). Table Table22 shows that deletions in 3/6 Salmonella-specific regions confer a defect in virulence as determined in a CI assay. A CI < 1.0 indicates that the mutant is less fit than the wild type. Strains MT2040, MT2037, and MT2055 exhibit a CI that is significantly reduced from 1.0 as determined by the t test (P < 0.05). Removal of the Class III iviXXII region (17 kb) conferred the largest virulence defect in BALB/c mice (>300-fold) and showed reduced survival (3.2-fold) in RAW 264.7 cultured murine macrophages. Removal of other ivi regions conferred smaller defects in mice. The additive effect of such small contributions might have a significant impact on fitness within the host and, over time, lead to the formation of distinct serovars and species with differing host range, tissue tropism, and disease manifestation.

Table 2
Deletions of Salmonella-specific regions confer virulence defects

DISCUSSION

We have employed IVET to identify six Salmonella regions of atypical base composition that are not present in many other species tested, including several pathogenic strains of E. coli. These Salmonella-specific regions fall into three classes with respect to their distribution within four Salmonella serovars of differing host range: Class I sequences are present in broad host-range and host-adapted Salmonella serovars; Class II are present in all but the host-adapted serovar, S. typhi; Class III are present only in broad host-range serovars and not S. typhi nor the limited host-range serovar, S. choleraesuis. Many of these Salmonella-specific regions are required for full virulence and are associated with mobile elements, such as phage and insertion sequences that may have contributed to their acquisition by horizontal transfer. The incremental acquisition of functions encoded within these regions may contribute to evolution of species, host range, and disease manifestation.

Recent studies on microbial speciation have estimated that up to 30% of the genome sequences may differ between the closely related species S. typhimurium and E. coli (1, 3, 25). These differences have been attributed to genomic flux; the loss and acquisition of genes after divergence from a common ancestor. Indeed, ≈25% of the more than 100 ivi genes recovered from the IVET selection in S. typhimurium have no homologue in the DNA database (4, 10). Moreover, we have shown that at least 10 of these ivi genes reside in 6 distinct regions of atypical base composition and removal of a portion of 3 of these regions conferred a virulence defect in a murine typhoid fever model.

It is expected that at least some of the in vivo-induced Salmonella-specific sequences encode functions responsible for the speciation and subsequent evolution of distinct Salmonella serovars. Sequences present in all Salmonella spp. (Class I) are likely to have been inherited early in the divergence of Salmonella, encoding functions that allow it to occupy niches that are unavailable to its nearest relatives, similar to the proposed inheritance of S. typhimurium pathogenicity island 1 (SPI-1) (39). Sequences not found in all serovars (Classes II and III) represent later acquisitions or losses of functions that reflect the lifestyle of each serovar, as is proposed for unique virulence regions within avian pathogenic E. coli, which are not present in E. coli K-12 (40). In our studies, all probes were derived from ivi genes in the broad host range S. typhimurium and thus, by definition, S. typhimurium is a member of all hybridization classes described here. Therefore, it should not be interpreted from our results that S. typhimurium contains every sequence that is specific to the salmonellae. Rather, it is expected that probes derived from ivi genes of other serovars would identify sequences not present in S. typhimurium; indeed, S. typhi contains 118 kb of a sequence that is not present in S. typhimurium (41).

Many of the Salmonella-specific regions encode predicted proteins with sequence similarity to virulence proteins from a wide variety of pathogens and removal of these regions confers a virulence defect. For example, Fig. Fig.11 (line 1 and legend) shows that the iviVI-A region encodes predicted products that show similarity to several adhesin-like molecules (10, 1215) and a lipopolysaccharide modification enzyme (16). The iviXVII region (Fig. (Fig.1,1, line 2) allows growth on 1,2-propanediol (24) and the virulence defect observed after its removal may reflect the significance of using alternative carbon sources in vivo. Moreover, the acquisition of this region in Salmonella spp. is thought to have contributed to its divergence from the closely related E. coli (1, 3). The iviXXII region (Fig. (Fig.1,1, line 6) contains phage-like functions, including ssrA, a tRNA-like structure that is part of the insertion site for the E. coli cryptic prophage CP4–57 (34) and the insertion site of a pathogenicity island in D. nodosus (35). Removal of a portion of this region conferred the greatest defect in virulence. Deletions of some Salmonella-specific regions did not show an effect in the murine infection model, but may encode functions that are either redundant or important for virulence in other hosts.

The association of pathogenicity islands with phage-like functions that integrate into the chromosome at phage attachment sites is well documented (5). These attachment sites are often within the well-conserved, omnipresent tRNA genes that may facilitate phage-mediated horizontal transfer of virulence functions by providing compatible integration sites within many recipient species. Moreover, the importance of functions encoded within integrated phages themselves to virulence has been demonstrated by the increased serum resistance in λ lysogens of E. coli (42). Along these lines, the recently sequenced E. coli genome reveals many cryptic phage functions that may enhance fitness, although the ability to produce lytic phages apparently has been lost (43).

From what organisms were these sequences acquired? The composition of acquired sequences will tend to drift over time (ameliorate) to the G + C content of the recipient (44), thus masking the identity of the donor organism. In cases where there is no DNA sequence homology, protein similarity may provide clues to the functions encoded by Salmonella-specific DNA, but not the source of the acquired sequence. For example, many Salmonella-specific DNA regions such as iviVI-A (Fig. (Fig.1,1, line 1) exhibit no homology to related species at the DNA level. However, the predicted IviVI-A gene product shows significant similarity over its entire length to enterotoxigenic E. coli Tia (10). This lack of DNA homology to other species suggests that the source of these acquired sequences remains to be identified. Perhaps they have been acquired from other pathogenic, commensal, or environmental organisms that shared similar animal or environmental reservoirs at some time during their evolutionary history.

The Salmonella-specific in vivo-induced regions comprise a fossil record of events that have lead to the evolution of distinct species and serovars. These acquired in vivo-selected sequences not only distinguish one pathogen from another but may also point to the functions involved in host/pathogen interactions, which lead to the host specificity and tissue tropism of each species, i.e., the functions that contribute to specific disease or carrier state caused by a given serovar in a given host. The elucidation of species-specific genes in clinically relevant bacteria may provide probes for the rapid and sensitive identification of infectious organisms.

Acknowledgments

We thank Ann Frazier for technical assistance, John R. Roth for the pdu deletion, Thomas A. Bobik for pdu sequence information before publication, and John Carbon for critically reviewing the manuscript. This work was supported by National Institutes of Health Grant AI36373, American Cancer Society Junior Faculty Research Award 544, and Beckman Young Investigator Award (M.J.M.).

ABBREVIATIONS

IVET
in vivo expression technology
CI
competitive index

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